Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (2): 334-342.doi: 10.3864/j.issn.0578-1752.2013.02.013

• HORTICULTURE • Previous Articles     Next Articles

Effects of Ultra-Dry Treatment on Vigor and Antioxidative Metabolism of Pugionium Seeds

 ZHAO  Peng, ZHANG  Yi-Ting, HAO  Li-Zhen, PANG  Jie, YANG  Zhong-Ren, ZHANG  Feng-Lan   

  1. College of Agriculture, Inner Mongolia Agricultural University/Inner Mongolia Autonomous Region Key Laboratory of Wild Peculiar Vegetable Germplasm Resource and Germplasm Enhancement, Huhhot 010019
  • Received:2012-08-09 Online:2013-01-15 Published:2012-10-29

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Abstract: 【Objective】 The effect of ultradry treatment on dynamic change of vigor, antioxidative metabolism of Pugionium. seeds were determined, which would provide a theoretical basis for desiccation tolerance and storage of the seeds. 【Method】The moisture content of P. cornutum(L.) Gaertn. and P. dolabratum Maxim. were reduced to 3.1%, 2.2%, 1.3% from 4.5% and 4.3% (CK), respectively, with silica gel, and the vigor and antioxidative metabolism were detected when the seeds were re-moistened. 【Result】 The vigor of P. dolabratum Maxim. seeds maintained at a relatively higher level, while the P. cornutum(L.) Gaertn. seeds decreased sharply when the moisture content dropped to 1.3% from respective CK. Relative electrical conductivity of the two species seeds with ultradry treatment were lower than the CK and the integrity of cell membranes was conserved. Ultradry treatment accelerated the generation of superoxide generation rate ( ) and malondialdehyde (MDA), but the treatment did not affect the activities of antioxidant enzymes of the two species seeds obviously such as SOD, CAT, GPX, APX. 【Conclusion】The results showed that both of the seeds of P. cornutum(L.) Gaertn. and P. dolabratum Maxim. had strong desiccation tolerance, however, the ability of resisting damage and the desiccation tolerance of P. dolabratum Maxim. seeds were stronger than P. cornutum(L.) Gaertn. seeds, and the moisture content of 2.2% was the turning point of germination and vigor of P. cornutum (L.) Gaertn. seeds.

Key words: P. cornutum(L.) Gaertn. , P. dolabratum Maxim. , seed , desiccation tolerance , antioxidative metabolism

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