Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (16): 3330-3336.doi: 10.3864/j.issn.0578-1752.2012.16.012

• HORTICULTURE • Previous Articles     Next Articles

Expression of Genes Related to Starch Synthesis During Rhizome Swelling of Lotus Root (Nelumbo nucifera Gaertn)

 CHENG  Li-Bao, LI  Shu-Yan, LI  Yan, YIN  Jing-Jing, CHEN  Xue-Hao, LI  Liang-Jun   

  1. 扬州大学园艺与植物保护学院，江苏扬州225009
  • Received:2011-11-01 Online:2012-08-15 Published:2012-06-20

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Abstract: 【Objective】 Critical genes relevant to starch synthesis were explored by studying gene expression during swelling of lotus rhizome. 【Method】 In this study, the changes of starch content and starch granule characteristics were studied during (cv. Mei Ren Hong) swelling period of lotus rhizome. Four important related genes were identified based on transcriptome analysis, digital gene expression profiling and qRT-PCR method. 【Result】 The result showed that content of the starch was improved more rapidly, and the starch granule was more enlarged at swelling stage than that at initial swelling stage. A total of 34 related genes to starch metabolism were identified during this process. However, only four genes including LrGBSS，LrSBEI，LrSBEII and LrSBEIII were obviously changed their transcriptional level at the swelling stage compared with initial swelling period. The result of qRT-PCR was further proved that LrGBSS，LrSBEI，LrSBEII and LrSBEIII showed a high correlation with starch synthesis according to their expression characteristics during rhizome development. 【Conclusion】The expression of LrGBSS，LrSBEI，LrSBEII and LrSBEIII is a critical factor to decide the starch content of lotus rhizome.

Key words: lotus root, rhizome, starch, gene expression, transcriptome

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