Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (16): 3330-3336.doi: 10.3864/j.issn.0578-1752.2012.16.012

• HORTICULTURE • Previous Articles     Next Articles

Expression of Genes Related to Starch Synthesis During Rhizome Swelling of Lotus Root (Nelumbo nucifera Gaertn)

 CHENG  Li-Bao, LI  Shu-Yan, LI  Yan, YIN  Jing-Jing, CHEN  Xue-Hao, LI  Liang-Jun   

  1. 扬州大学园艺与植物保护学院，江苏扬州225009
  • Received:2011-11-01 Online:2012-08-15 Published:2012-06-20

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Abstract: 【Objective】 Critical genes relevant to starch synthesis were explored by studying gene expression during swelling of lotus rhizome. 【Method】 In this study, the changes of starch content and starch granule characteristics were studied during (cv. Mei Ren Hong) swelling period of lotus rhizome. Four important related genes were identified based on transcriptome analysis, digital gene expression profiling and qRT-PCR method. 【Result】 The result showed that content of the starch was improved more rapidly, and the starch granule was more enlarged at swelling stage than that at initial swelling stage. A total of 34 related genes to starch metabolism were identified during this process. However, only four genes including LrGBSS，LrSBEI，LrSBEII and LrSBEIII were obviously changed their transcriptional level at the swelling stage compared with initial swelling period. The result of qRT-PCR was further proved that LrGBSS，LrSBEI，LrSBEII and LrSBEIII showed a high correlation with starch synthesis according to their expression characteristics during rhizome development. 【Conclusion】The expression of LrGBSS，LrSBEI，LrSBEII and LrSBEIII is a critical factor to decide the starch content of lotus rhizome.

Key words: lotus root, rhizome, starch, gene expression, transcriptome

[1]Du H, Zhao X, You J S, Park J Y, Kim S H, Chang K J. Antioxidant and hepatic protective effects of lotus root hot water extract with taurine supplementation in rats fed a high fat diet. Journal of Biomedical Science, 2010, 17 (Suppl. 1): S39.

[2]Masuda J I, Ozaki Y, Okubo H. Rhizome transition to storage organ is under phytochrome control in lotus (Nelumbo nucifera). Planta, 2007, 226 (4): 909-915.

[3]Liu J, Zhang M, Wang S. Processing characteristics and flavour of full lotus root powder beverage. Journal of the Science of Food and Agriculture, 2010, 90: 2482-2489.

[4]Slocum P D, Robinson P. Water gardening, water lilies and lotuses. Portland, OR: Timber Press, 1996.

[5]Zhang H Y, Dong S T, Gao R Q, Li Y Q. Comparison of starch synthesis and related enzyme activities in developing grains among different types of maize. Journal of Plant Physiology and Molecular Biology, 2007, 33 (1): 25-32.

[6]Le C D, Bras J, Dufresne A. Starch nanoparticles. Biomacromolecules, 2010, 11 (5): 1139-1163.

[7]Chen M H, Bergman C, Pinson S, Fjellstrom R. Wasy gene haplotypes: Associations with apparent amylase content and effect by the environment in and international rice germplasm collection. Journal of Cereal Science, 2008, 47: 536-545.

[8]Resurreccion A P, Hara T, Juliano B O, Yoshida S. Effects of temperatures during ripening on grain quality of rice. Soil Science and Plant Nutrition, 1977, 23: 109-112.

[9]Dobo M, Ayres N, Walker G, Park W D. Polymorphism in the GBSS gene affects amylase content in US and European rice germplasm. Journal of Cereal Science, 2010. 52 (3): 450-456.

[10]Hennen-Bierwagen T A, Liu F, Marsh R S, Kim S, Gan Q G, Tetlow L J, James M G, Myers A M. Starch biosynthetic enzymes from developing maize endosperm association in multisubunit complexes. Plant Physiology, 2008, 146 (4): 1892-1908.

[11]Regina A, Kosar-hashemi B, Ling S, Li Z, Rahman S, Morell M. Control of starch branching in barley defined through differential RNAi suppression of starch branching enzyme IIa and Iib. Journal of Experimental Botany, 2010, 61 (5):1469-1482.

[12]Kuipers A G J, Jacobsen E, Visser R G F. Formation and deposition of amylase in the potato tuber starch granule are affected by the reduction of granule-bound starch synthase gene expression. The Plant Cell, 1994, 6 (1): 43-52. 

[13]Vlachos N A, Karapantsios. Water content measurement of thin sheet starch products using a conductance technique. Journal of Food Engineering, 2000, 46 (2): 91-98.

[14]Mu-forster C, Huang R, Powers J R, Harriman W R, Knight M, Singletary G W, Keeling P L, Wasserman B P. Physical association of starch biosynthetic enzymes with starch granules of maize endosperm. Plant Physiology, 1996, 111 (3): 821-829.

[15]Jeng T L, Tseng T H, Wang C S, Chen C L, Sung J M. Starch biosynthesizing enzymes in developing grains of rice cultivar Tainung 67 and its sodium azide-induced rice mutant. Field Crop Research, 2003, 84 (3): 261-269.

[16]Hirose T, Teral T. A comprehensive expression analysis of the starch synthase gene family in rice (Oryza sativa L.). Planta, 2004, 220 (1): 9-16.

[17]Denyer K, Smith A M. The purification and characterization of the two forms of soluble starch synthase from developing pea embryos. Planta, 1992, 186 (4): 609-617.

[18]Burton R A, Bewley J D, Smith A M, Bhattacharyya M K, Tatge H, Ring S, Bull V, Hamilton W D, Martin C. Starch branching enzymes belonging to distinct enzyme families is differentially expressed during pea embryo development. Plant Journal, 1995, 7 (1): 3-15.

[19]Wttebled F, Buleon A, Bouchet B, Ral J P, Lienard L, Delvalle D, Binderup K, Dauvillee D, Ball Steven Dhulst C. Granule-bound starch synthesis I, a major enzyme involved in the biogenesis of B-crystallites in starch granules. European Journal of Biochemistry, 2002, 269 (15): 3810-3820.

[20]Hanashiro I, Itoh K, Kuratomi Y, Yamazaki M, Igarashi T, Matsugasako J I, Takeda Y. Granule-Bound starch synthase I is responsible for biosynthesis of extra-long unit chains of amylopectin in rice. Plant Cell Physiology, 2008, 49 (6): 925-933.

[21]Nakamura T, Vrinten P, Hayakawa K, Ikeda J. Characterization of a granule-bound starch synthase isoform found in the pericarp of wheat. Plant Physiology, 1998, 118 (2): 451-459. [22]Marshall J, Sidebottom C, Debet M, Marlin C, Smith A M, Edwards  A. Identification of the major starch synthase in the soluble fraction of potato tubers. The Plant Cell, 1996, 8 (1): 1121-1135.

[23]Guan H P, Preiss J. Differentiation of the properties of the branching isozymes (Zea mays). Plant Physiology, 1993, 102 (4): 1269-1273.

[24]Rahman A, Wong K S, Jane J L, Myers A M, James M. Characterization of SU1 isoamylase, a determinant of storage starch structure in maize. Plant Physiology, 1998, 117 (2): 425-435.

[25]Kubo A, Fujita N, Harada K, Matsuda T, Satoh H, Nakamura Y. The starch-debranching Enzymes isoamylase and pullulanase are both involved in amylopectin biosynthesis in rice endosperm. Plant Physiology, 1999, 121 (2): 399-410.

[26]James M G, Robertson D S, Myers A M. Characterization of the maize gene sugary1, a determinant of starch composition in kernels. The Plant Cell, 1995, 7 (4): 417-429.

[27]Zhu Z P, Hylton C M, Rössner U, Smith A M. Characterization of starch-debranching enzymes in pea embryos. Plant Physiology, 1998, 118 (2): 581-590.

[28]Nakamura Y, Umemoto T, Takahata Y, Komae K, Amano E, Satoh H. Changes in the structure of starch and enzyme activities effected by sugary mutation in developing rice endosperm. Possible role of starch debranching enzyme (R-enzyme) in amylopectin biosynthesis. Physiologia Plantarum, 1996, 97 (3): 491-498.

[29]Cheng F M, Zhong L J, Zhao N C, Liu Y, Zhang G P. Temperature induced changes in the starch components and biosynthetic enzyme of two rice varieties. Plant Growth Regulation, 2005, 46 (1): 87-95.

[30]Schwall G P, Safford R, Westcott R J, Jeffcoat R, Tayal A, Shi Y C, Gidley M J, Jobling S A. Production of very-high-amylose potato starch by inhibition of SBE A and B. Nature Biotechnology, 2000, 18: 551-554.

[31]Maddelein M L. Toward an understanding of the biogenesis of the starch granule: determination of granule bound and soluble starch synthase functions in amylopectinsynthesis. Journal of Biological Chemistry, 1994, 269 (40): 25150-25157.

[32]Reeves C D, Krishnan H B, Okita T W. Gene expression in developing wheat endosperm. Plant Physiology, 1986, 82 (1): 34-40.
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