Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (16): 3231-3239.doi: 10.3864/j.issn.0578-1752.2012.16.001

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Functional Analysis via Overexpressing Xyloglucan Endotransglycosylase Gene OsXTH11 in Rice

 ZHANG  Li, NIU  Xiang-Li, ZHANG  Hui-Ying, LIU  Yong-Sheng   

  1. 1.重庆大学农学及生命科学研究院，重庆 400000
    2.合肥工业大学生物与食品工程学院，合肥 230009
  • Received:2012-03-26 Online:2012-08-15 Published:2012-05-16

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Abstract: 【Objective】 The functions of rice XTH gene (OsXTH11) in deveolpment regulation and stress responses were investigated by utilizing transgenic technology. 【Method】 The overexpression vector of OsXTH11 was constructed and introduced into rice cv. Nipponbare by Agrobacterium-mediated transformation. The phenotypes analysis and molecular detection of transgenic plants were performed to verify the function of OsXTH11. 【Result】 Real-time PCR indicated that the expression levels of OsXTH11 in transgenic lines were distinctly increased. The performance of T1 transgenic seedlings revealed that OsXTH11 overexpression lines showed increased growth rates under normal condition, and more responsive to gibberellin (GA) and auxin (IAA). The stem elongation of transgenic plants was significantly faster than that of wild type under dark or deep-water conditions, and exhibited enhanced tolerance to 100 mmol•L-1 NaCl. 【Conclusion】 Phenotypes of transgenic plants overexpressed the xyloglucan endotransglycosylase/hydrolase gene suggested the role of OsXTH11 in rice growth and salt stress.

Key words: Oryza sativa L., xyloglucan endotransglucosylase/hydrolase, phytohormone, salt stress

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