Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (14): 2981-2990.doi: 10.3864/j.issn.0578-1752.2012.14.022

• VETERINARY SCIENCE • Previous Articles     Next Articles

Isolation, Identification and Bioinformatics Analysis of CAT Protein Related with Hepatotoxity by Copper Nanoparticles in Rats

 DONG  Shu-Wei, GAO  Zhao-Hui, SHEN  Xiao-Yun, XUE  Hui-Wen, LI  Xia   

  1. 1.中国农业科学院兰州畜牧与兽药研究所/农业部兽用药物创制重点实验/甘肃省中兽药工程技术中心，兰州 730050
    2.贵州省毕节学院，贵州毕节 551700
    3.甘肃农业大学动物医学院，兰州 730070
  • Received:2012-01-19 Online:2012-07-15 Published:2012-04-01

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Abstract: 【Objective】In order to investigate the hepatotoxic mechanisms of nanoparticles copper, catalase (CAT) was isolated and identified from liver, and analyzed by bioinformatics, which is related with hepatotoxity induced by copper nanoparticles in rats.【Method】 The differential expression proteins related with hepatotoxity of copper nanoparticles were screened by 2-DE and PDQuest 8.0 software and then analyzed by bioinformatics after identified by MALDI-TOF-TOF MS through comparative proteomics strategy.【Result】The 6602 and 7702 spots of differentially expressed proteins were found to associate with hepatotoxity. They were identified as CAT protein which was located in the cytoplasm. This hydrophilic protein had no signal peptide, and was non-secreted protein. It also contained catalase active sites 64FDRERIPERVVHAKGAG80 and catalase heme ligand sites 354RLFAYPDTH362. The random coils, α-helices and extended chains were its main secondary structural elements, and the three-dimensional structure was predicted. Homology analysis showed that CAT had a high homology between rat and the other eight species, and the phylogenetic tree of CAT was constructed. 【Conclusion】Copper nanoparticles could regulate down the CAT protein expression so as to induce oxidative stress injury in liver cells, which may be a pathway of copper nanoparticles to exert the hepatotoxic effects in rats.

Key words: copper nanoparticles, hepatotoxity, proteomics, catalase, bioinformatics, rat

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