Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (14): 2973-2980.doi: 10.3864/j.issn.0578-1752.2012.14.021

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

The Base Mutation of Ovine UCP1 Gene and Its Relationship   with Protein Structure and Function

 YUAN  Ya-Nan, LIU  Wen-Zhong, LIU  Jian-Hua, QIAO  Li-Ying, WU  Jian-Liang   

  1. 山西农业大学动物科技学院，山西太谷 030801
  • Received:2011-11-17 Online:2012-07-15 Published:2012-01-30

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Abstract: 【Objective】The objectives of this study are to detect the single nucleotide polymorphism (SNP) of UCP1 gene in two fat-tailed sheep breeds, and to predict the protein structure and function caused by the mutations. 【Method】 PCR-SSCP combined with sequencing were used to detect the SNPs in coding region of UCP1 gene, and bioinformatics approach was used to predict the physicochemical properties and structures of UCP1 protein. 【Result】 Four SNPs existed in the coding region, with c.214G＞A (Val72Met) and c.273C＞T located on exon 2, c.624C＞T and and c.757G＞A (Ala253Thr) on exon 5. Further analysis indicated that the two mutations which caused amino acid substitution had little influence on the protein expression. This was because of the two mutations merely resulted in a slight change of the physicochemical properties and transcription factor binding site, but did not lead to the change of its spatial configuration. 【Conclusion】Comparison of UCP1 protein structures between two exonic mutants, which associated respectively with human obesity and diabetes, and the normal structure suggested that the changes in UCP1 protein structure could influence the protein function.

Key words: sheep, uncoupling protein 1 (UCP1), single nucleotide polymorphism (SNP), bioinformatics, protein structure

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