芝麻茎点枯病抗性关联分析及抗病载体材料挖掘

doi:10.3864/j.issn.0578-1752.2012.13.003

Abstract

Abstract: 【Objective】Developing molecular markers related with resistance to Macrophomina phaseolina from natural population, identifying elite resistant accessions. 【Method】In this study, 216 sesame core collections were scanned by 79 pairs of EST-SSR, SRAP and AFLP primers, accurate identification of their resistance to Macrophomina phaseolina was carried out from 2009 to 2011, and based on these data the genes related with resistance to M. phaseolina were located by association analysis. 【Result】A total of 608 polymorphic bands were amplified, population structure analysis indicated that it was consisted of 2 subgroups, 43 markers significantly associated with M. phaseolina disease index simultaneously in 2 or 3 years were detected by association analysis based on GLM(Q) and MLM(Q+K) model, explained 1.82% to 9.46% of phenotypic variation, 3 associated markers were detected by both of 2 models, and marker M7E6-1 was presented simultaneously in 3 years. Ten accessions with resistance to M. phaseolina were identified, the coincident rate of 43 associated markers in the 10 accessions was 60% to 100%, with an average of 92.09%. 【Conclusion】 The tested population was consisted of 2 subgroups. There was no relationship between division of subgroup and geographical origin of the material, 43 markers were detected significantly and stably associated with M. phaseolina disease index. There was a diverse variation among the resistance to M. phaseolina of the tested population, and 10 accessions with stable resistance to M. phaseolina were identified.

Key words: sesame, Macrophomina phaseolina, association mapping, resistant accession, identification

ZHANG Yan-Xin, WANG Lin-Hai, LI Dong-Hua, WEI Wen-Liang, GAO Yuan, ZHANG Xiu-Rong. Association Mapping of Sesame (Sesamum indicum L.) Resistance to Macrophomina phaseolina and Identification of Resistant Accessions[J].Scientia Agricultura Sinica, 2012, 45(13): 2580-2591.

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Association Mapping of Sesame (Sesamum indicum L.) Resistance to Macrophomina phaseolina and Identification of Resistant Accessions

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