Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (9): 1807-1813.doi: 10.3864/j.issn.0578-1752.2012.09.016

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Effects of Transportation on Serum Proteome in Dairy Cows

 YUAN  Ting-Jie, WANG  Jia-Qi, YANG  Yong-Xin, BU  Deng-Pan, YANG  Jin-Hui, ZHOU  Ling-Yun   

  1. 1.中国农业科学院北京畜牧兽医研究所/动物营养学国家重点实验室，北京 100193
    2.甘肃农业大学动物科学技术学院，兰州  730070
  • Received:2011-09-07 Online:2012-05-01 Published:2011-12-21

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Abstract: 【Objective】The experiment was designed to investigate the effects of transport on serum proteome of dairy     cows. 【Method】Proteome analysis in serum from dairy cows on the 7 d before transport , at 3 h and on the 7 d after transport were performed using two-dimensional electrophoresis (2-DE) coupled with MADIL-TOF-TOF mass spectrum. 【Result】 Fourteen protein spots having significantly different expression levels after transport were observed. Serum albumin, Ig gamma 1 heavy chain constant region, type II cytoskeletal 1 and transthyretin were down-regulated at 3 h after transport, while alpha 1-acid glycoprotein, haptoglobin, nesprin-2-like, microtubule-actin cross-linking factor 1, tyrosine-protein kinase Fps85D, exportin 5 and an unknown protein spot were up-regulated comparative with the 7 d pre-transport. With the exception of alpha 1-acid glycoprotein, the expression volume of these proteins on the 7 d after transport were not different with those on the 7 d before transport. 【Conclusion】These proteins are involved in acute phase response, immune response, transport as well as various metabolic pathways in cells, suggesting transport resulted in stress.

Key words: transport, dairy cow, serum, proteome

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