铝胁迫下钙信号抑制剂对外生菌根真菌分泌草酸的影响

doi:10.3864/j.issn.0578-1752.2012.05.010

Abstract

Abstract: 【Objective】 An experiment was carried out to study the mechanism of oxalate effluxes by ectomycorrhizal fungi (ECMF) under Al stress and to establish a theoretical foundation for alleviating the Al toxicity of trees, guide the selection of Al-resisted ECMF and preserve forest health. 【Method】 The growth and the oxalate effluxes by ectomycorrhizal species, Suillus luteus (Sl 08 and Sl 14 ) and Boletus sp. (Bo 15), which were isolated from acid forest soil, were studied in pure culture in liquid media with variable concentrations of Al3+ and Ca2+ signal inhibitors. 【Result】 Compared to the control without Al3+, when Al3+ concentration was 0.4 mmol•L-1, the growth of Sl 14 was inhibited significantly. But the growth of Sl 08 and Bo 15 was inhibited significantly in 1.2 mmol•L-1 Al3+ culture. It was showed that Sl 14 was sensitive to Al; Sl 08 and Bo 15 were resistant to Al. Meanwhile, the oxalate efflux by Sl 14 was few and invariable with the increase of Al3+ concentration in culture. But the oxalate effluxes by Sl 08 and Bo 15 increased with the increase of Al3+ concentration in the range of 0.2-0.8 mmol•L-1 Al3+ culture. It showed that the oxalate effluxes by ECMF were closely related with the tolerance of ECMF to Al. In the culture without Al3+, compared to without Ca2+ signal inhibitors, there was no significant change in the oxalate efflux by Bo 15 in the respective presence of verapamil (VP), ruthenium red (RR), trifluoperazine (TFP) and ethylene glycol tetraacetic acid (EGTA), while the oxalate efflux by Sl 08 was decreased significantly in the respective presence of VP, TFP, and EGTA. So Ca2+ signals could regulate oxalate efflux by Sl 08 but not by Bo 15 in culture without Al3+. Under Al stress, however, the oxalate effluxes by Sl 08 and Bo 15 were reduced significantly by four Ca2+ signal inhibitors. It was showed that Ca2+ signals could regulate oxalate efflux by Sl 08 and Bo 15 under Al stress. Oxalate efflux by Bo 15 was most sensitive to TFP, which led oxalate effluxes to a most greatly reduction, and Sl 08 was most sensitive to RR and TFP, compared to any other Ca2+ signal inhibitors.【Conclusion】 These results showed that Ca2+ signal participated in oxalate effluxes to accommodate Al stress in the fungi.

Key words: ECMF, Al, oxalate, Ca2+ signal

WANG Ming-Xia, ZHOU Zhi-Feng, YUAN Ling, HUANG Jian-Guo. Effects of Ca2+ Signal Inhibitors on Oxalate Effluxes by Ectomycorrhizal Fungi Under Aluminum Stress[J].Scientia Agricultura Sinica, 2012, 45(5): 902-908.

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Effects of Ca2+ Signal Inhibitors on Oxalate Effluxes by Ectomycorrhizal Fungi Under Aluminum Stress

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