Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (5): 902-908.doi: 10.3864/j.issn.0578-1752.2012.05.010

• SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT • Previous Articles     Next Articles

Effects of Ca2+ Signal Inhibitors on Oxalate Effluxes by Ectomycorrhizal Fungi Under Aluminum Stress

 WANG  Ming-Xia, ZHOU  Zhi-Feng, YUAN  Ling, HUANG  Jian-Guo   

  1. 1.西南大学资源环境学院，重庆 400716
  • Received:2011-09-13 Online:2012-03-01 Published:2011-12-31

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Abstract: 【Objective】 An experiment was carried out to study the mechanism of oxalate effluxes by ectomycorrhizal fungi (ECMF) under Al stress and to establish a theoretical foundation for alleviating the Al toxicity of trees, guide the selection of Al-resisted ECMF and preserve forest health. 【Method】 The growth and the oxalate effluxes by ectomycorrhizal species, Suillus luteus (Sl 08 and Sl 14 ) and Boletus sp. (Bo 15), which were isolated from acid forest soil, were studied in pure culture in liquid media with variable concentrations of Al3+ and Ca2+ signal inhibitors. 【Result】 Compared to the control without Al3+, when Al3+ concentration was 0.4 mmol•L-1, the growth of Sl 14 was inhibited significantly. But the growth of Sl 08 and Bo 15 was inhibited significantly in 1.2 mmol•L-1 Al3+ culture. It was showed that Sl 14 was sensitive to Al; Sl 08 and Bo 15 were resistant to Al. Meanwhile, the oxalate efflux by Sl 14 was few and invariable with the increase of Al3+ concentration in culture. But the oxalate effluxes by Sl 08 and Bo 15 increased with the increase of Al3+ concentration in the range of 0.2-0.8 mmol•L-1 Al3+ culture. It showed that the oxalate effluxes by ECMF were closely related with the tolerance of ECMF to Al. In the culture without Al3+, compared to without Ca2+ signal inhibitors, there was no significant change in the oxalate efflux by Bo 15 in the respective presence of verapamil (VP), ruthenium red (RR), trifluoperazine (TFP) and ethylene glycol tetraacetic acid (EGTA), while the oxalate efflux by Sl 08 was decreased significantly in the respective presence of VP, TFP, and EGTA. So Ca2+ signals could regulate oxalate efflux by Sl 08 but not by Bo 15 in culture without Al3+. Under Al stress, however, the oxalate effluxes by Sl 08 and Bo 15 were reduced significantly by four Ca2+ signal inhibitors. It was showed that Ca2+ signals could regulate oxalate efflux by Sl 08 and Bo 15 under Al stress. Oxalate efflux by Bo 15 was most sensitive to TFP, which led oxalate effluxes to a most greatly reduction, and Sl 08 was most sensitive to RR and TFP, compared to any other Ca2+ signal inhibitors.【Conclusion】 These results showed that Ca2+ signal participated in oxalate effluxes to accommodate Al stress in the fungi.

Key words: ECMF, Al, oxalate, Ca2+ signal

[1]Cumming J R, Weinstein L H. Aluminum-mycorrhizal interactions in the physiology of pitch pine seedlings. Plant and Soil, 1990, 125: 7-18.

[2]Ahonen-Jonnarth U, Göransson A, Finlay R D. Growth and nutrient uptake of ectomycorrhizal Pinus sylvestris seedlings in a natural substrate treated with elevated Al concentrations. Tree Physiology, 2003, 23: 157-167.

[3]Schier G A, Mcquattie C J. Effect of nitrogen source on aluminum toxicity in nonmycorrhizal and ectomycorrhizal pitch pine seedling. Journal of Plant Nutrition, 1999, 22:951-965.

[4]辜夕容, 梁国仕, 杨水平, 陈翠玲, 黄建国. 接种双色蜡蘑对马尾松幼苗生长, 养分和抗铝性的影响. 林业科学, 2005, 41(4): 199-203.

Gu X R, Liang G S, Yang S P, Chen C L, Huang J G. Influences of Laccaria bicolor on the growth, nutrient uptake and aluminum resistance of pinus massoniana seedlings. Scientia Silvae Sinicae, 2005, 41(4): 199-203. (in Chinese)

[5]Klugh-Stewart K, Cumming J R. Organic acid exudation by mycorrhizal Andropogon virginicus L. (broomsedge) roots in response to aluminum. Soil Biology and Biochemistry, 2009, 41:367-373.

[6]Ray P, Adholeya A. Correlation between organic acid exudation and metal uptake by ectomycorrhizal fungi grown on pond ash in vitro. Biometals, 2009, 22:275-281.

[7]Cumming J R, Swiger T D, Kurnik B S, Panaccione D G. Organic acid exudation by Laccaria bicolor and Pisolithus tinctorius exposed to aluminum in vitro. Canadian Journal of Forest Research, 2001, 31: 703-710.

[8]辜夕容, 黄建国. 铝对外生菌根真菌草酸分泌及磷, 钾, 铝吸收的影响. 生态学报, 2010, 30(2): 357-363.

Gu X R, Huang J G. Effect of aluminum on growth, oxalate exudation, and uptake of aluminum, phosphorus and potassium by ectomycorrhizal fungi in vitro. Acta Ecologica Sinica, 2010, 30(2): 357-363. (in Chinese)

[9]Munir E, Yoon J J, Tokimatsu T, Hattori T, Shimada M. A physiological role for oxalic acid biosynthesis in the wood-rotting basidiomycete Fomitopsis palustris. Proceedings of the National Academy of Sciences of the United States of America, 2001, 98: 11126-11130.

[10]Reddy V S, Reddy A S N. Proteomics of calcium signaling components in plants. Phytochemistry, 2004, 65: 1745-1776.

[11]Rengel Z, Zhang W H. Role of dynamics of intracellular calcium in aluminium-toxicity syndrome. New Phytologist, 2003, 159: 295-314.

[12]Sivaguru M, Yamamoto Y, Rengel Z, Ahn S J, Matsumoto H. Early events responsible for aluminum toxicity symptoms in suspension- cultured tobacco cells. New Phytologist, 2005, 165: 99-109.

[13]Ma J F, Ryan P R, Delhaize E. Aluminium tolerance in plants and the complexing role of organic acids. Trends in Plant Science, 2001, 6: 273-278.

[14]Ryan P R, Skerrett M, Findlay G P, Delhaize E, Tyerman S D. Aluminum activates an anion channel in the apical cells of wheat roots. Proceedings of the National Academy of Sciences of the United States of America, 1997, 94: 6547-6552.

[15]Gelli A, Higgins V J, Blumwald E. Activation of plant plasma membrane Ca2+ permeable channels by lace. specific fungal elicitors. Plant Physiology, 1997, 113: 269-279.

[16]唐新莲, 黎晓峰, 凌桂芝, 顾明华, 玉永雄. Ca2+ 信号参与铝诱导黑麦根系分泌有机酸的调控. 中国农业科学, 2008, 41(8): 2279-2285.

Tang X L, Li X F, Ling G Z, Gu M H, Yu Y X. The involvement of Ca2+ signal in the regulation of Al-induced secretion of organic acids in rye. Scientia Agricultura Sinica, 2008, 41(8): 2279-2285. (in Chinese)

[17]van Heesa P A W, Vinogradoffb S I, Edwardsc A C, Godboldb D L, Jones D L. Low molecular weight organic acid adsorption in forest soils: effects on soil solution concentrations and biodegradation rates. Soil Biology and Biochemistry, 2003, 35: 1015-1026.

[18]Dutton M V, Evan C S. Oxalate production by fungi: its role in pathogenicity and ecology in the soil environment. Canadian Journal of Microbiology, 1996, 42:881-895.

[19]Liu Y Y, Guo S X. Involvement of Ca2+ channel signalling in sclerotial formation of polyporus umbellatus. Mycopathologia, 2010, 169: 139-150.

[20]李焕秀. 生长素运输抑制剂和钙拮抗剂对菜豆下胚轴生长素诱导的导管分化和形成的影响. 四川农业大学学报, 1993, l1(2): 233- 237.

Li H X. The effects of auxin transport inhibttor and calcium antagonisits on auxin-induced tracheary differentiation and formation of bean hypocotyls. Journal of Sichuan Agricultural University, 1993, 11(2): 233-237. (in Chinese)

[21]Heim A, Brunner I, Frossard E, Luster J. Aluminum effects on picea abies at low solution concentrations. Soil Science Society of America Journal, 2003, 67: 895-898.

[22]Schier G A, McQuattie C J. Response of ectomycorrhizal and nonmycorrhizal pitch pine (Pinus rigida) seedlings to nutrient supply and aluminum: growth and mineral nutrition. Canadian Journal of Forest Research, 1996, 26: 2145-2152.

[23]Lapeyrie F, Chilvers G A, Bhem C A. Oxalic acid synthesis by the mycorrhizal fungus Paxillus involutus (Batsch. ex Fr.) Fr. New Phytologist, 1987, 106: 139-146.

[24]袁 玲, 方德华, 汪智慧, 魏兴元, 黄建国. 钾对外生茵根真菌的分泌作用及氮, 磷, 钾含量的影响. 生态学报, 2001, 21(2):254-258.

Yuan L, Fang D H, Wang Z H, Wei X Y, Huang J G. Effects of potassium on the secretion of proton and oxalate by ectomycorrhizal fungi and the concentrations of nitrogen, phosphorus and potassium in their hyphae. Acta Ecologica Sinica, 2001, 21(2): 254-258. (in Chinese)

[25]Ahonen-Jonnarth U, van Hees P A W, Lundström U S, Finlay R D. Organic acids produced by mycorrhizal Pinus sylvestris exposed to elevated aluminium and heavy metal concentrations. New Phytologist, 2000, 146: 557-567.

[26]Ma Q, Rengel Z, Kuo J. Aluminium toxicity in rye (Secale cereale): root growth and dynamics of cytoplasmic Ca2+ in intact root tips. Annals of Botany, 2002, 89: 241-244.

[27]王建波, 李阳生, 利容千. 铝胁迫下小麦根尖分生细胞中Ca2+分布变化. 生态学报, 2001, 21(8): 1246-1250.

Wang J B, Li Y S, Li R Q. Changes of Ca2+ distribution in root apical meristematic cells of wheat under aluminum stress. Acta Ecologica Sinica, 2001, 21(8): 1246-1250. (in Chinese)

[28]Zhang W H, Ryan P R, Tyerman S D. Malate-permeable channels and cation channels activated by aluminum in the apical cells of wheat roots. Plant Physiology, 2001, 125: 1459-1472.
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