Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (12): 2558-2565.doi: 10.3864/j.issn.0578-1752.2013.12.017

• ANIMAL SCIENCE·VETERINARY SCIENCERE • Previous Articles     Next Articles

Polymorphism of Chicken Prolactin Receptor Gene and It’s Association with Broodiness and Egg Reproduction Traits

 DU  Xiao-Hui, LI  Cong-Yan, LIU  Jun-Ying, NIU  Wei-He, ZHANG  Xi-Quan   

  1. 1.College of Animal Science, Sichuan Agricultural University, Ya’an 625014, Sichuan
    2.Sichuan Animal Science Academy,   Chengdu 610066
    3.College of Animal Science, South China Agricultural University, Guangzhou 510642.
  • Received:2013-03-26 Online:2013-06-15 Published:2013-05-08

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Abstract: 【Objective】 In order to reveal the relationship between bloodiness and egg reproduction of Chinese indigenous chicken and prolactin receptor (PRLR) gene,which closely related to reproductive traits among amimals.【Method】Using the PCR-SSCP method and 576 Ningdu Yellow chicken, the experiment studied the polymorphism of PRLR gene and its association with nesting and egg production traits.【Result】The results confirmed previous studies that SNP1 (T10862C, exon 3) and SNP2 (T25670C, exon 6) had no significant association with broody traits (P＞0.05). The effect of SNP2 on egg production of 300 d (EN-300 d) was significantly (P＜0.05). The new SNP3 (G30716A, intron 8) significantly influenced the age at first egg (AFG) (P＜0.05). The novel SNP4 (A31900G, exon 10) had an extremely remarkable (P＜0.01) impact on the nesting rate, significant (P＜0.05) impact on the total broody days and EN-300 d. The differences among different haplotypes constructed by the four SNPs, in AFG reached an extremely significant level (P＜0.01), in other traits were no significant differences (P＞0.05). 【Conclusion】 PRLR gene polymorphism was associated with broodiness and egg production traits, especially the effect of SNP4.

Key words: Ningdu yellow chicken , PRLR gene , polymorphism , broodiness , egg production traits

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