Scientia Agricultura Sinica ›› 2017, Vol. 50 ›› Issue (18): 3608-3619.doi: 10.3864/j.issn.0578-1752.2017.18.016

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Transcriptome Analysis of Differential Gene Expression Associated with Testis Tissue in Mule Duck and Muscovy Duck

LI Li1, MIAO ZhongWei1, XIN QingWu1, ZHU ZhiMing1, ZHANG LinLi1, ZHUANG XiaoDong2, ZHENG NenZhu1,3   

  1. 1Institute of Animal Science and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013; 2Fujian Changlong Group, Zhangzhou 363000, Fujian; 3Food college of Fujian Agriculture and Forestry University, Fuzhou 350002
  • Received:2017-02-04 Online:2017-09-16 Published:2017-09-16

RichHTML

2

PDF

410

Abstract: 【Objective】 The purpose of this study is to analyze the transcriptome differential gene expression of mule duck and muscovy duck testis, results of the study will lay a theoretical foundation for the further elucidation of the mechanisms of genetic sterility of mule duck. 【Method】 Transcriptome sequencing of testis from mule duck and muscovy duck was performed using the Illumina HiSeq 2500 platform with 2 biological replicates per duck breed, and verified by quantitative real-time PCR (QRT-PCR). 【Result】 After removing sequencing adaptors and the low-quality reads, a total of 43.84 Gb clean reads were obtained, the Q30 base percentage at 91.36% and above, and clean reads were assembled into 193 535 Unigene. The r2 Differential expression analysis showed that 3 597 differentially expressed genes were found between two duck breeds, including 1 194 up regulated genes and 2 403 down-regulated genes. Several genes were related with reproductive function, such as fibroblast growth factor, FGF, protein kinase A (PKA), mitogen-activated protein kinase 7-like, partial (BMK),growth factor receptor-bound protein 2 (GRB2), c-Jun N-terminal kinase (JNK )and tumor necrosis factor receptor superfamily member 6(FAS),and so on. With Gene Ontology (GO) annotation, 382 differentially expressed genes were identified including 97 related annotation genes involving development and reproduction biological process. Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis showed that differentially expression genes annotated to 50 metabolic pathway, and 17 pathways were enriched significantly, such as calcium signaling pathway, glycerolipid metabolism and MAPK signaling pathway, and gonadotropin releasing hormone (GnRH) signaling pathway and MAPK signaling pathway associated with testis physiology and reproduction activities. Verified by QRT-PCR, the pattern of differential gene expression was consistent with the results of transcriptome sequencing, which showed the sequencing results were reliable. 【Conclusion】 Differentially expressed genes of mule duck and muscovy duck testis were screened by transcriptional analysis, revealed that the GnRH and MAPK signaling pathway play an important role in duck reproductive activities, which provide reliable reference for exploring the differentiation mechanism of reproductive system in mule duck.

Key words: mule duck, muscovy duck, testis tissue, differentially expressed genes, transcriptome

[1]    檀俊秩, 陈晖, 宋建捷, 刘玉涛. 半番鸭繁殖性状的研究. 福建农业学报, 1998, 13(2): 41-45.
TAN J Z, CHEN H, SONG J J, LIU Y T. Studies on reproductive character of mule duck. Journal of Fujian Agricultural Sciences, 1998, 13(2):41-45. (in Chinese)
[2]    刘军须, 蔡月花, 张敬各, 张福英. 雄性不育大鼠近交系MIJ的建立及其遗传特征观察. 动物学杂志, 2008, 43(5) :37-44.
LIU J X, CAI Y H, ZHANG J G, ZHANG F Y. Establishment     and genetic characteristics of rat inbred strain MIJ with spontaneous male infertility. Journal of Animal Science, 2008, 43(5):37-44. (in Chinese)
[3]    张庆波, 李齐发, 李家璜, 李新福, 刘振山, 潘增祥, 宋大伟, 谢庄. 牛精子发生相关新基因b-DAZL的克隆、生物信息学分析与组织表达研究. 自然科学进展, 2008, 18(5):493-504.
ZHANG Q B, LI Q F, LI J H, LI X F, LIU Z S, PAN Z X, SONG D W, XIE Z. Cloning, bioinformatics analysis and tissue expression of a novel b-DAZL gene related to spermatogenesis in cattle. Progress in Natural Science, 2008, 18(5):493-504. (in Chinese)
[4]    LI S J, WANG C, YU W H, ZHAO S H, GONG Y Z. Identification of genes related to white and black plumage formation by RNA-Seq from white and black feather bulbs in ducks. PloS One, 2012, 7: e36592.
[5]    LI Q H, WANG N, DU Z, HUX X, CHENL, FEI J, WANGY Y, LI. N. Gastrocnemius transcriptome analysis reveals domestication induced gene expression changes between wild and domestic chickens. Genomics, 2012, 100: 314-319.
[6]    陈黎, 黄学涛, 田勇, 陶争荣, 卢立志. 利用转录组测序筛选与鸭青壳性状形成相关的基因. 农业生物技术学报, 2016, 24(7): 1064-1072.
CHEN L, HUANG X T, TIAN Y, TAO Z R, LU L Z. Identifying genes associated with blue eggshell in ducks (Anasplatyrhynchos domesticus) by transcriptome analysis. Journal of Agricultural Biotechnology, 2016, 24(7): 1064-1072. (in Chinese)
[7]    BAUERSACHS S, WOLF E. Transcriptome analyses of bovine, porcine and equine endometrium during the pre-implantation phase[J]. Animal reproduction science. 2012,134:84-94.
[8]    FIEDLER T J, HUDDER A, MCKAY S J, SHIVKUMAR S, CAPO T R, SCHMALE M C, WALSH P J. The transcriptome of the early life history stages of the california sea hare aplysia californica, comparative biochemistry and physiology Part D. Genomics & Proteomics, 2010, 5:165-170.
[9]    张伟. 中华绒螯蟹精巢组织文库构建和基因的克隆与序列分析[D]. 上海: 华东师范大学, 2012.
ZHANG W. Construction of cDNA library of Chinese mitten crab, eriocheir sinensis ：East China Normal University, 2012. (in Chinese)and cloeing and molecular characterization of gene[D]. Shanghai
[10]   钟志君. 猪睾丸和卵巢组织转录组的差异分析[D]. 雅安：四川农业大学, 2012 .
ZHONG Z J. Repertoire of porcine microRNA in adult ovary and testis by deep sequencing[D]. Sichuan: Sichuan Agricultural University, 2012 (in Chinese)
[11]   张升利, 付成东, 梁拥军, 李文通, 孙砚胜, 史东杰, 张 欣. 长尾草金鱼成熟期雌雄性腺RNA-Seq转录组分析. 水产科学, 2014, 33(12): 750-753.
ZHANG S L, FU C D, LIANG Y J, LI W T, SUN Y S, SHI D J, ZHANG X. The RNA-Seq transcriptome analysis in male gonads of Long-tailed goldfish rassius auratus. Fisheries Science, 2014, 33(12): 750-753. (in Chinese)
[12]   Xu Q, Zhao W M, Chen Y, Tong Y Y, Rong G H, Huang Z Y, Zhang Y, Chang G B, Wu X S, Chen G H. Transcriptome profiling of the goose (Anser cygnoides) ovaries identify laying and broodiness phenotypes. PloS One, 2013, 8:e55496.
[13]   朱志明, 陈红萍, 林如龙, 缪中纬, 辛清武, 李丽, 张丹青, 郑嫩珠. 山麻鸭开产期和产蛋高峰期卵巢组织转录组分析. 中国农业科学, 2016, 49(5):998-1007.
ZHU Z M, CHEN H P, LIN R L, MIAO Z W, XIN Q W, LI L, ZHANG D Q, ZHENG N Z. Transcriptome analysis of ovary tissue in early laying period and egg laying peak period of shanma Ducks. Scientia Agricultura Sinica, 2016, 49(5):998-1007. (in Chinese)
[14]   SCHULZE S K, KANWAR R, GÖLZENLEUCHTER M, THERNEAU T M, BEUTLER A S. SERE: Single-parameter quality control and sample comparison for RNA-Seq. [BMC genomics Italic], 2012, 13(1): 524.
[15]   XIANG L X, HE D, DONG W R, ZHANG Y W, SHAO J Z.  Deep sequencing-based transcriptome profiling analysis of bacteria challenged Lateolabrax japonicus reveals insight into the immune-relevant genes in marine fish. BMC Genomics, 2010, 11: 472.
[16]   WANG B, GUO G W, WANG C, LIN Y, WANG X M, ZHAO M M, GUO Y, HE M H, ZHANG Y, PAN L. Survey of the transcriptome of aspergillus oryzae via massively parallel mRNA sequencing. Nucleic Acids Research, 2010, 38(15): 5075-5087.
[17]   LI M Y, TAN H W, WANG F, JIANG Q, XU Z S, TIAN C, XIONG A S. De novo transcriptome sequence assembly and identification of AP2/ERF transcription factor related to abiotic stress in parsley (Petroselinum crispum). PLoS One, 2014, 9(9): e108977.
[18]   ZHU S Y, TANG S M, TANG Q M, LIU T M. Genome-wide transcriptional changes of ramie (Boehmeria nivea L. Gaud) in response to root-lesion nematode infection. Gene, 2014, 552: 67-74.
[19]   CONG F, LIU X L, HAN Z X, SHAO Y H, KONG X P, LIU S W. Transcriptome analysis of chicken kidney tissues following coronavirus avian infectious bronchitis virus infection. Genomics, 2013, 14: 743-756.
[20]   FRASER B A, WEADICK C J, JANOWITZ I, RODD F H, HUGHES K A. Sequencing and characterization of the guppy (Poecilia reticulata) transcriptome. BMC Genomics, 2011, 12:202.
[21]   LI G Y, XIE P, LI H Y, HAO L, XIONG Q, QIU T.Involment of p53, Bax, and Bcl-2 pathway in microcystins-induced apoptosis in rat testis. Environmental Toxicology, 2011, 26(2):111-117.
[22]   郑炜, 赵宗胜, 李青峰, 班谦, 李洪涛, 梁耀伟. Solexa测序技术分析鸡与鹌鹑属间杂交雌性和雄性胚胎的差异microRNAs. 中国兽医学报, 2014, 34(1):116-122.
ZHENG W, ZHAO Z S, LI Q F, BAN Q, LI H T, LIANG H T. Analysis of difference microRNAs in female and male embryos of chicken-quail hybrid by solexa sequencing. Chinese Journal of Veterinary Science, 2014, 34(1):116-122. (in Chinese)
[23]   DRUMMOND A E. TGFβ signaling in the development of ovarian function. Cell and Tissue Research, 2005, 322(l): 107-115.
[24]   KONRAD L, KEILANI M M, LAIBLE L, NOTTELMANN U, HOFMANN R. Effects of TGF-betas and a specific antagonist on apoptosis of immature rat male germ cells in vitro. Apoptosis, 2006, 11(5): 739-748.
[25]   SUN Q Y, BREITBART H, SCHATTEN H. Role of the MAPK cascade in mammalian germ cells. Reproduction, Fertility, and Development, 1999, 11(8):443-450.
[26]   HARRIS V K, COTICCHIA C M, KAGAN B L, AHMAD S, WELLSTEIN A, RIEGE A T, HARRIS V K, COTICCHIA C M. Induction of the angiogenic modulator fibroblast growth factor- binding protein by epidermal growth factor is mediated through both MEK/ERK and p38 signal transduction path ways. Journal Biology Chemistry, 2000, 275(15): 10802-10811.
[27]   ALMOG T, NAOR Z. Mitogen activated protein kinases (MAPKs) as regulators of spermatogenesis and spermatozoa functions. Molecular and Cellular Endocrinology, 2008, 282:39-44.
[28]   JOHNSON C, JIA Y, WANG C, LUE Y H, SWERDLOFF R S, ZHANG X S, HU Z Y, LI Y C, LIU Y X, AMIYA P. Sinha Hikim. Role of Caspase 2 in apoptotic signaling in primate and murine germ cells. Biology of Reproduction, 2008, 79 (5): 806.
[29]   SHOW M D, HILL C M, ANWAY M D, WRIGHT W W, ZIRKIN B R. Phosphorylation of mitogen-activated protein kinase 8 (MAPK8) is associated with germ cell apoptosis and redistribution of the Bcl2- Modifying Factor (BMF). Journal of Andrology, 2008，29(3): 338-344.
[30]   VALVE E, PENTTILA T L, PARANKO J, HA¨RKO¨NEN P. FGF-8 is expressed during specific phases of rodent oocyte and spermatogonium development. Biochemical and Biophysical Research Communications, 1997, 232(1) :173-177.
[31]   DVORAK P, DVORAKOV D, HAMPL A. Fibroblast growth factor signaling in embryonic and cancer stem cells. FEBS Letters , 2006, 580: 2869-2874.
[32]   MIALON A, SANKINEN M, SO¨DERSTRO¨M H, JUNTTILA T T, HOLMSTRO¨M T, KOIVUSALO R, PAPAGEORGIOU A C, JOHNSON R S, HIETANEN S, ELENIUS K, WESTERMARCK J. DNA topoisomerase I is a cofactor for c-jun in the regulation of epidermal growth factor receptor expression and cancer cell proliferation. Molecular and Cellular Biology, 2005, 12(25): 5040-5051.
[33]   陈丽莉,肖亚梅,刘文彬,赵如榕,刘姣,刘筠,李万程.ERK/JNK在黄鳝雌、雄发育阶段生殖腺中的表达和定位.动物学报，2007, 53(2): 325-331.
CHEN L L, XIAO Y M, LIU W B, ZHAO R R, LIU J, LI W C. Expression and location of ERK/JNK in ovary and spermary in rice field eels monopterus albus. Journal of Animal Science, 2007, 53(2):325-331. (in Chinese)
[34]   SHEIKH A Q, TAGHIAN T, HEMINGWAY B, CHO H, KOGAN A B, NARMONEVA1 D A. Regulation of endothelial MAPK/ERK signalling and capillary morphogenesis by low-amplitude electric field. Journal of the Royal Society Interface, 2013, 10(78): 1-13.
[35]   Vien H Y Lee, Leo T O Lee, Billy K C Chow. Gonadotropin- releasing hormone: regulation of the GnRH gene. FEBS Journal, 2008, 275: 5458-5478.
[36]   BELOV A A, MOHAMMADI M. Grb2, a double-edged sword of receptor tyrosine kinase signaling. Science Signaling, 2012, 5(249): 49.
[37]   HAINES E, MINOO P, FENG Z Q, RESALATPANAH N, NIE X M, CAMPIGLIO M, ALVAREZ L, COCOLAKIS E, RIDHA M, FULVIO M D, GOMEZ-CAMBRONERO J, LEBRUN J J, ALI S. Tyrosine phosphorylation of Grb2:Role in prolactin /epidermal growth factor cross talk in mammary epithelial cell growth and differentiation. Molecular and Cellular Biology, 2009, 29(10):2505-2520.
[38]   YUAN Y Y, CHEN W Y, SHI Q X, MAO L Z,YU S Q, FANG X, ROLDAN E R S. Zona pellucida induces activation of phospholipase A2 during acrosomal exocytosis in guinea pig spermatozoa. Biology of Reproduction, 2003, 68(3): 904-913.
[39]   SANTI C M, SANTOS T, HERNÁNDEZ-CRUZ A, DARSZON A. Properties of a novel pH-dependent Ca2+ permeation pathway present in male germ cells with possible roles in spermatogenesis and mature sperm function. The Journal of General Physiology, 1998,112: 33-53.
[1] LIU Kai,HE ShanShan,ZHANG CaiXia,ZHANG LiYi,BIAN ShuXun,YUAN GaoPeng,LI WuXing,KANG LiQun,CONG PeiHua,HAN XiaoLei. Identification and Analysis of Differentially Expressed Genes in Adventitious Shoot Regeneration in Leaves of Apple [J]. Scientia Agricultura Sinica, 2021, 54(16): 3488-3501.
[2] LIU Yuan,LI WenYang,WU XianFeng,HUANG QinLou,GAO ChengFang,CHEN XinZhu,ZHANG XiaoPei. Transcriptome Analysis of Differentially Gene Expression Associated with longissimus doris Tissue in Fuqing Goat and Nubian Black Goat [J]. Scientia Agricultura Sinica, 2019, 52(14): 2525-2537.
[3] WANG DanDan, TANG YuTing, MA YueHui, WANG LiGang, PAN DengKe, JIANG Lin. Studying the Molecular Mechanism of Heart Development by Using ZBED6 Gene Knockout Pig [J]. Scientia Agricultura Sinica, 2018, 51(7): 1390-1400.
[4] LIU HongXiang,XU WenJuan,ZHU ChunHong,TAO ZhiYun,SONG WeiTao,ZHANG ShuangJie,LI HuiFang. RNA-seq Analysis on Development Arrest of Duck Pectoralis Muscle During Semi-Late Embryonic Period [J]. Scientia Agricultura Sinica, 2018, 51(22): 4373-4386.
[5] SUN LiNa, TIAN ZhiQiang, ZHANG HuaiJiang, LI YanYan, YAN WenTao, YUE Qiang, QIU GuiSheng. Transcriptome Analysis of Disruption of Mating in the Peach Fruit Moth (Carposina sasakii) by Chlorantraniliprole [J]. Scientia Agricultura Sinica, 2018, 51(15): 2925-2936.
[6] LIU HongBo, LIU XinLong, SU HuoSheng, LU Xin, XU ChaoHua, MAO Jun, LIN XiuQin, LI ChunJia, LI XuJuan, ZI QiuYan. Transcriptome Difference Analysis of Saccharum spontaneum Roots in Response to Drought Stress [J]. Scientia Agricultura Sinica, 2017, 50(6): 1167-1178.
[7] PENG FuZhi, RAN MaoLiang, WENG Bo, LI Zhi, DONG LianHua, CHEN Bin. Validation of Reference Genes for Quantitative RT-PCR Analysis in Porcine Testis Tissues [J]. Scientia Agricultura Sinica, 2017, 50(15): 3033-3041.
[8] ZHU Zhi-ming, CHEN Hong-ping, LIN Ru-long, MIAO Zhong-wei, XIN Qing-wu, LI Li, ZHANG Dan-qing, ZHENG Nen-zhu. Transcriptome Analysis of Ovary Tissue in Early Laying Period and Egg Laying Peak Period of Shanma Ducks [J]. Scientia Agricultura Sinica, 2016, 49(5): 998-1007.
[9] GENG Li-ying, PAN Su-min, CHEN Juan, ZHU Wen-jin, GONG Yuan-fang, LIU Zheng-zhu, PENG Yong-dong, ZHAO Shu-yu, ZHANG Chuan-sheng, LI Xiang-long . Identification and Bioinformatics Analysis of Differential Expression MicroRNAs in the Spleen Between Beijing Fatty Chickens and Leghorns Chickens [J]. Scientia Agricultura Sinica, 2016, 49(4): 754-764.
[10] YUE Xiao-feng, QUE Ya-wei, WANG Zheng-yi. Analysis of RNA-Seq-Based Expression Profiles of Δznf1 Mutants in Magnaporthe oryzae [J]. Scientia Agricultura Sinica, 2016, 49(17): 3347-3358.
[11] CHENG Chun-Zhen-12, ZENG Ji-Wu-2, BEI Xue-Jun-12, WU Bo-12, YANG Jia-Wei-3, ZHANG Yong-Yan-12, JIANG Bo-2, ZHU Shi-Ping-1, YAN Shu-Tang-1, ZHONG Yun-2, ZHONG Guang-Yan-2. Screening of ‘Jincheng’ Orange CTV Response Genes Using Subtractive Suppression Hybridization [J]. Scientia Agricultura Sinica, 2013, 46(16): 3413-3423.
[12] XIE Zheng-Lu, SHEN Xue-Huai, LIU Lin, YIN Fu-Jian, MA Hai-Tian, FAN Hong-Jie. Construction of Subtractive Hybridization cDNA Library to Screen Differentially Expressed Genes from the Spleen of C57BL/6 and A/J Mouse and Their Functional Analysis [J]. Scientia Agricultura Sinica, 2012, 45(7): 1406-1417.
[13] ZHANG Yuan-Yue, HE Xi. Multilevel Nonlinear Mixed Effect Model for Evaluation of   Muscovy Ducks Body Weight for Growth [J]. Scientia Agricultura Sinica, 2012, 45(3): 540-547.
[14] ZHENG Nen-Zhu, CHEN Xiao-Yan, LU Li-Zhi, ZHU Zhi-Ming, MIAO Zhong-Wei, XIN Qing-Wu, CHEN Hui, XIAO Tian-Fang. Construction and Identification of Normalized Subtractive cDNA Library of Plumage Color-Related Genes in Mule Duck [J]. Scientia Agricultura Sinica, 2012, 45(22): 4688-4696.
[15] WU Xu, YAN Mei-Jiao, LIU Li-Ping, FU Xing-Yuan, LIAN Sen-Yang, LI Ang. cDNA-AFLP Analysis on Transcripts Associated Gene with Broodiness in Muscovy Duck [J]. Scientia Agricultura Sinica, 2012, 45(2): 353-358.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd