Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (16): 3413-3423.doi: 10.3864/j.issn.0578-1752.2013.16.012

• HORTICULTURE • Previous Articles     Next Articles

Screening of ‘Jincheng’ Orange CTV Response Genes Using Subtractive Suppression Hybridization

 CHENG  Chun-Zhen-12, ZENG  Ji-Wu-2, BEI  Xue-Jun-12, WU  Bo-12, YANG  Jia-Wei-3, ZHANG  Yong-Yan-12, JIANG  Bo-2, ZHU  Shi-Ping-1, YAN  Shu-Tang-1, ZHONG  Yun-2, ZHONG  Guang-Yan-2   

  1. 1.College of Horticulture and Landscape Architecture/Citrus Research Institute, Southwest University, Chongqing 400715
    2.Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou 510640
    3.Economic Crops Station,  Agricultural Bureau of Rongxian, Zigong 643100, Sichuan
  • Received:2013-03-25 Online:2013-08-15 Published:2013-05-27

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Abstract: 【Objective】The objective of this study is to make clear the molecular mechanism and screen citrus tristeza virus (CTV) response genes in CTV susceptive citrus species ‘Jincheng’ orange [Citrus sinensis (L) Osbeck].【Method】 A suppression subtractive hybridization (SSH) library of ‘Jincheng’ orange was successfully constructed by using the virus-free leaves as Driver and those infected with CTV as Tester. 【Result】After sequencing of the randomly picked 850 clones, 742 clones were successfully sequenced and a total of 692 ESTs were obtained after Vectorscreen and adaptors deletion. All these ESTs were then matched to citrus genome transcripts and a total of 137 genes were identified. BLAST2GO gene ontology and KEGG (Kyoto Encyclopedia of Genes and Genomes) analysis results showed that the basic energy metabolisms of ‘Jincheng’ orange were significantly influenced with differentially expressed genes (DEGs) involving in carbon fixation in photosynthetic organisms, nitrogen metabolism, starch and sucrose metabolism, etc. Moreover, DEGs largely involved in defense and resistance and some plant defensins synthesis related pathways such as carotenoid biosynthesis, phenylpropanoid biosynthesis, and phenylalanine metabolism. 【Conclusion】Results generated in this study suggested that CTV susceptible species ‘Jincheng’ orange reacted severely to CTV infection to maintain its growth and development by elevating energy metabolisms and enhancing the expression of stress resistance related genes.

Key words: citrus , suppression subtractive hybridization (SSH) , differentially expressed genes (DEGs) , CTV

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