利用cDNA-AFLP技术筛选菊花开花相关基因

doi:10.3864/j.issn.0578-1752.2011.16.011

Abstract

Abstract: 【Objective】Differentially expressed transcripts of the apical buds in chrysanthemum induced by different photoperiod were analyzed and to isolate the flowering related genes.【Method】cDNA-AFLP technique was used to identify differentially expressed transcript-derived fragments (TDFs) in the apical buds of chrysanthemum induced by different photoperiods of long-day (16h/8h, day/night) and short-day (8h/16h, day/night) treatments, and the TDFs were validated, cloned, sequenced and the bioinformation analyzed. 【Result】 A total of 2 917 TDFs were screened by 64 primer combinations, and 835 TDFs were identified differentially expression, including 584 TDFs up regulation and 251 TDFs down regulation. Fifty TDFs were successfully cloned and the sequence was analyzed. The results indicated that 36 TDFs had homology in NCBI database, and 30 of which displayed homology to genes with known functions, 5 no had a match. Functional analysis indicated that 31 TDFs mainly participated in 7 kinds of processes of signal transduction, development, transcription regulation, protein degradation and synthesis, metabolism, stress responses etc. Semi-quantitative RT-PCR analysis with selected transcripts of 6 flowering related genes indicated that the 6 flowering related genes all differentially expressed or up regulated under the short-day conditions, while they all did not express or down regulate under the long-day conditions. These results were similar to those of cDNA-AFLP expression patterns in chrysanthemum.【Conclusion】In this study several flowering related gene pigments were selected by cDNA-AFLP techniques. These novel genes could be used in future for functional analysis and strategies of molecular breeding.

Key words: Chrysanthemum, cDNA-AFLP, TDFs, floral differentiation, flowering related genes

REN Hong-Yan, SUN Xia, ZHENG Cheng-Shu, WANG Wen-Li, SUN Xian-Zhi, SHU Huai-Rui. Differential Analysis of Flowering Related Genes by cDNA-AFLP in Chrysanthemum[J].Scientia Agricultura Sinica, 2011, 44(16): 3386-3394.

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Differential Analysis of Flowering Related Genes by cDNA-AFLP in Chrysanthemum

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