Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (10): 1980-1988.doi: 10.3864/j.issn.0578-1752.2011.10.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Overlap Between Salt and Low-Temperature Tolerance Loci at Germination Stage of Soybean

 QIU  Peng-Cheng, ZHANG  Wen-Bo, JIANG  Hong-Wei, LIU  Chun-Yan, LI  Can-Dong, FAN  Dong-Mei, ZENG  Qing-Li, HAN  Dong-Wei, HU  Guo-Hua, CHEN  Qing-Shan   

  1. 1.黑龙江省农垦科研育种中心，哈尔滨 150090
    2.东北农业大学农学院，哈尔滨 150030
    3.国家大豆工程技术研究中心，哈尔滨 150050
    4.黑龙江省农业科学院佳木斯分院，黑龙江佳木斯 154007
    5.黑龙江省农业科学院齐齐哈尔分院，黑龙江齐齐哈尔 161006
  • Received:2010-09-16 Online:2011-05-15 Published:2010-11-26

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Abstract: 【Objective】The main purpose of this paper was to map the QTLs related with salt tolerance and low-temperature tolerance at germination stage with backcross introgression lines (ILs), and analyze the genetic overlap between them. These overlapping QTL loci could be used to breed soybean varieties with both salt tolerance and low-temperature tolerance, improve efficiency of stress-tolerance breeding. 【Method】A primary backcross introgression lines (ILs) were constructed with Hong-feng11 as recurrent parent and Harosoy as donor parent. Then 48 individuals from BC2F4 introgression populations were obtained after screening in 1.75% NaCl solution, and 40 individuals were screened out after 6℃ low-temperature treatment comparing with the Hong-feng11 at germination stage. The QTL identification of the germination stage was conducted by chi-square test and analysis of variances with the two introgression populations. 【Result】There were 22 QTL located with BC2F4 introgression populations after salt stress, and 15 QTL were mapped after low-temperature stress. Seven overlapping QTLs between salt tolerance and low-temperature tolerance were detected on 6 linkage groups at germination stage. 【Conclusion】In total, there were 31.81% of salt tolerance and low-temperature tolerance loci existed genetic overlap.

Key words: soybean, introgression lines, salt tolerance, low-temperature tolerance, genetic overlap

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