Abstract:

【Objective】 In order to understand differential expression proteins in the response to wheat powder mildew between two near-isogenic wheat lines, BAINONG 3217, which is susceptive to wheat powder mildew, and W2132-6, which carrys resistance gene Pm21 and is resistant to wheat powder mildew. 【Method】 Differential proteomics between the two lines were studied after they were infected by Blumeria graminis. Protein samples were respectively extracted from the leaves after 48 h inoculation, separated by two-dimensional electrophoresis, dyed by CBB, and were identified by MALDI-TOF-MS. 【Result】 It was found that there were twelve protein spots expressed differentially, and nine of them were identified by MALDI-TOF-MS and database searching. The predicted function of seven proteins were related to energy metabolism, signal control, defensive responses and proteins stabilization, which were involved in many resistant physiological reactions, including enhancing energy metabolism, signal control, antioxidant reaction, cell-wall reinforcement and lignification. 【Conclusion】 The response to powder mildew was changed by Pm21. Stress response proteins (e.g. proline-rich family protein) should be related with resistance to wheat power mildew.

Key words: differential proteomics, wheat, powder mildew, Pm21