猪POU1F1第一内含子单核苷酸多态性和生长性状相关性研究

doi:10.3864/j.issn.0578-1752.2009.01.036

Scientia Agricultura Sinica ›› 2009, Vol. 42 ›› Issue (1): 283-289 .doi: 10.3864/j.issn.0578-1752.2009.01.036

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles Next Articles

Relationship of Single Nucleotide Polymorphism of Intron 1 in Procine POU1F1 and the Growth Trait

中国农业科学院北京畜牧兽医研究所

Received:2008-01-28 Revised:2008-04-27 Online:2009-01-10 Published:2009-01-10
Contact: ZHENG You-min

Abstract

Abstract:

【Objective】 The relationship between porcine DNA polymorphisms of POU1F1 and mRNA expression was studied. 【Method】 The intron 1, a partial regional polymorphisms of porcine POU1F1 of two breeds of Yorkshare and Zhongxu black, were investigated by PCR-SSCP. Based on the total RNA contracted from animals pituitary, the mRNA quantities of POU1F1, GH, PRL, and TSH-? were masured by RQ-PCR. 【Result】 It was found that a SNP of T→C in intron 1 (site 1515bp), markedly correlated with the daily weight gain (R=-0.6, P＜0.05) and the expression of GH (P＜0.05) before slaughtering. 【Conclusion】The SNP of T→C led to the increase of the GH expression; But the daily weight gain decreasd in the growth trait before slaughtering.

Key words: POU1F1, PCR-SSCP, polymorphisms, SNP

. Relationship of Single Nucleotide Polymorphism of Intron 1 in Procine POU1F1 and the Growth Trait
[J].Scientia Agricultura Sinica, 2009, 42(1): 283-289 .

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