Abstract: High molecular weigh glutenin subunits (HMW-GS) 7+8* was introduced into Longmai 20(1,17+18,5+10) by 5 consecutive backcrosses with biochemical marker assisted selection from a Canadian extra strong wheat cultivar Glenlea which possessing an overexpression of the Bx7 subunit. The near isogenic lines (NILs) of HMW-GS were obtained and grown in the experimental field of Crop Breeding Institute in 2005. The experimental design was a randomized block with six replicates. As compared to NILs with 17+18 subunit, the average of quality data of NILs with 7+8* subunit were 5 %（P =0.012）、4%（P =0.018）、10% (P =0.013)、13%（P=0.258）、256%（P =0.029） and 86%（P =0.020） higher in flour protein content、dry gluten content、Zeleny sedimentation、development time、stability and breakdown time, respectively, and were 2%（P =0.033）and 35%（P=0.007）lower in wet gluten/dry gluten and degree of softening, respectively. The application of subunits 7+8* in wheat breeding for quality was discussed in this study.

Key words: Wheat, Quality, HMW-GS, 7+8* subunits, Near-Isogenic Lines(NILs)