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Special Issue:
园艺-分子生物合辑Horticulture — Genetics · Breeding
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Genome-wide identification and expression analysis of anthocyanin biosynthetic genes in Brassica juncea |
ZHANG Da-wei1, 2, LIU Li-li1, 2, ZHOU Ding-gang1, 2, LIU Xian-jun3, LIU Zhong-song4, YAN Ming-li1, 2 |
1 School of Life Science, Hunan University of Science and Technology, Xiangtan 411201, P.R.China
2 Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization, Xiangtan 411201, P.R.China
3 College of Life Sciences, Resources and Environment Sciences, Yichun University, Yichun 333600, P.R.China
4 Oilseed Research Institute, Hunan Agricultural University, Changsha 410128, P.R.China |
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Abstract Anthocyanins confer the wide range of colors for plants and also play beneficial health roles as potentially protective factors against heart disease and cancer. Brassica juncea is cultivated as an edible oil resource and vegetable crop worldwide, thus elucidating the anthocyanin biosynthetic pathway would be helpful to improve the nutritional quality of Brassica juncea through the breeding and cultivating of high anthocyanin content varieties. Herein, 129 genes in B. juncea were identified as orthologs of 41 anthocyanin biosynthetic genes (ABGs) in Arabidopsis thaliana by comparative genomic analyses. The B. juncea ABGs have expanded by whole genome triplication and subsequent allopolyploidizatoin, but lost mainly during the whole genome triplication between B. rapa/B. nigra and A. thaliana, rather than the allopolyploidization process between B. juncea and B. rapa/B. nigra, leading to different copy numbers retention of A. thaliana homologous genes. Although the overall expansion levels ABGs were similar to the whole genome, more negative regulatory genes were retained in the anthocyanin biosynthesis regulatory system. Transcriptional analysis of B. juncea with different anthocyanin accumulation showed that BjDFR, BjTT19, BjTT8 are significantly up-regulated in plants with purple leaves as compared with green leaves. The overexpression of BjTT8 and these target genes which were involved in late anthocyanin biosynthesis and transport might account for increasing levels of anthocyanin accumulation in purple leaves. Our results could promote the understanding of the genetic mechanism of anthocyanin biosynthesis in B. juncea.
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Received: 12 July 2019
Accepted:
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| Fund: This work was funded by the National Key Research and Development Program of China (2016YFD0100202), the Natural Science Foundation of Hunan Province, China (2016JJ1010) and the Scientific Research Fund of Hunan Provincial Education Department, China (18C0305, 17K035, 17C0652, and 17C0653). |
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Corresponding Authors:
Correspondence YAN Ming-li, Tel/Fax: +86-731-58291416, E-mail: ymljack@126.com
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| About author:
ZHANG Da-wei, E-mail: zhangdawei.hnust@foxmail.com; |
Cite this article:
ZHANG Da-wei, LIU Li-li, ZHOU Ding-gang, LIU Xian-jun, LIU Zhong-song, YAN Ming-li.
2020.
Genome-wide identification and expression analysis of anthocyanin biosynthetic genes in Brassica juncea . Journal of Integrative Agriculture, 19(5): 1250-1260.
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Appelhagen I, Jahns O, Bartelniewoehner L, Sagasser M, Weisshaar B, Stracke R. 2011. Leucoanthocyanidin dioxygenase in Arabidopsis thaliana: Characterization of mutant alleles and regulation by MYB-BHLH-TTG1 transcription factor complexes. Gene, 484, 61–8.
Buer C S, Imin N, Djordjevic M A. 2010. Flavonoids: New roles for old molecules. Journal of Integrative Plant Biology, 52, 98–111.
Butelli E, Titta L, Giorgio M, Mock H P, Matros A, Peterek S, Schijlen E G, Hall R D, Bovy A G, Luo J, Martin C. 2008. Enrichment of tomato fruit with health-promoting anthocyanins by expression of select transcription factors. Nature Biotechnolgy, 26, 1301–1308.
Chen S, Wan Z, Nelson M N, Chauhan J S, Redden R, Burton W A, Lin P, Salisbury P A, Fu T, Cowling W A. 2013. Evidence from genome-wide simple sequence repeat markers for a polyphyletic origin and secondary centers of genetic diversity of Brassica juncea in China and India. Journal of Heredity, 104, 416–427.
Cheng F, Wu J, Fang L, Wang X. 2012. Syntenic gene analysis between Brassica rapa and other Brassicaceae species. Frontiers in Plant Science, 3, 198.
Cheng J, Liao L, Zhou H, Gu C, Wang L, Han Y. 2015. A small indel mutation in an anthocyanin transporter causes variegated colouration of peach flowers. Journal of Experimental Botany, 66, 7227–7239.
Fu W, Chen D, Pan Q, Li F, Zhao Z, Ge X, Li Z. 2018. Production of red-flowered oilseed rape via the ectopic expression of Orychophragmus violaceus OvPAP2. Plant Biotechnolgy Journal, 16, 367–380.
Guo N, Cheng F, Wu J, Liu B, Zheng S, Liang J, Wang X. 2014. Anthocyanin biosynthetic genes in Brassica rapa. BMC Genomics, 15, 426.
Guo N, Wu J, Zheng S, Cheng F, Liu B, Liang J, Cui Y, Wang X. 2015. Anthocyanin profile characterization and quantitative trait locus mapping in zicaitai (Brassica rapa L. ssp. chinensis var. purpurea). Molecular Breeding, 35, 113.
Kim J, Lee W J, Vu T T, Jeong C Y, Hong S W, Lee H. 2017. High accumulation of anthocyanins via the ectopic expression of AtDFR confers significant salt stress tolerance in Brassica napus L. Plant Cell Reports, 36, 1215–1224.
Lepiniec L, Debeaujon I, Routaboul J M, Baudry A, Pourcel L, Nesi N, Caboche M. 2006. Genetics and biochemistry of seed flavonoids. Annual Review of Plant Biology, 57, 405–430.
Li H, Zhu L, Yuan G, Heng S, Yi B, Ma C, Shen J, Tu J, Fu T, Wen J. 2016. Fine mapping and candidate gene analysis of an anthocyanin-rich gene, BnaA.PL1, conferring purple leaves in Brassica napus L. Molecular Genetics and Genomics, 291, 1523–1534.
Li P, Chen B, Zhang G, Chen L, Dong Q, Wen J, Mysore K S, Zhao J. 2016. Regulation of anthocyanin and proanthocyanidin biosynthesis by Medicago truncatula bHLH transcription factor MtTT8. New Phytologist, 210, 905–921.
Liu X, Lu Y, Yuan Y, Liu S, Guan C, Chen S, Liu Z. 2013. De novo transcriptome of Brassica juncea seed coat and identification of genes for the biosynthesis of flavonoids. PLoS ONE, 8, e71110.
Liu X, Lu Y, Yan M, Sun D, Hu X, Liu S, Chen S, Guan C, Liu Z. 2016. Genome-wide identification, localization, and expression analysis of proanthocyanidin-associated genes in Brassica. Frontiers in Plant Science, 7, 1831.
Mushtaq M A, Pan Q, Chen D, Zhang Q, Ge X, Li Z. 2016. Comparative leaves transcriptome analysis emphasizing on accumulation of anthocyanins in Brassica: Molecular regulation and potential interaction with photosynthesis. Frontiers in Plant Science, 7, 311.
Padmaja L K, Agarwal P, Gupta V, Mukhopadhyay A, Sodhi Y S, Pental D, Pradhan A K. 2014. Natural mutations in two homoeologous TT8 genes control yellow seed coat trait in allotetraploid Brassica juncea (AABB). Theoretical and Applied Genetics, 127, 339–347.
Sun Y, Li H, Huang J R. 2012. Arabidopsis TT19 functions as a carrier to transport anthocyanin from the cytosol to tonoplasts. Molecular Plant, 5, 387–400.
Wang X, Wang H, Wang J, Sun R, Wu J, Liu S, Bai Y, Mun J H, Bancroft I, Cheng F, Huang S, Li X, Hua W, Wang J, Wang X, Freeling M, Pires J C, Paterson A H, Chalhoub B, Wang B. 2011. The genome of the mesopolyploid crop species Brassica rapa. Nature Genetics, 43, 1035–1039.
Xu W, Grain D, Bobet S, Le Gourrierec J, Thévenin J, Kelemen Z, Lepiniec L, Dubos C. 2014. Complexity and robustness of the flavonoid transcriptional regulatory network revealed by comprehensive analyses of MYB-bHLH-WDR complexes and their targets in Arabidopsis seed. New Phytologist, 202, 132–144.
Xu W, Grain D, Le Gourrierec J, Harscoët E, Berger A, Jauvion V, Scagnelli A, Berger N, Bidzinski P, Kelemen Z, Salsac F, Baudry A, Routaboul J M, Lepiniec L, Dubos C. 2013. Regulation of flavonoid biosynthesis involves an unexpected complex transcriptional regulation of TT8 expression, in Arabidopsis. New Phytologist, 198, 59–70.
Yan M, Liu X, Guan C, Chen X, Liu Z. 2010. Cloning and expression analysis of an anthocyanidin synthase gene homolog from Brassica juncea. Molecular Breeding, 28, 313–322.
Yan M, Liu X, Guan C, Liu L, Xiang J, Lu Y, Liu Z. 2014. Cloning of TTG1 gene and PCR identification of genomes A, B and C in Brassica species. Genetica, 142, 169–176.
Yang J, Liu D, Wang X, Ji C, Cheng F, Liu B, Hu Z, Chen S, Pental D, Ju Y, Yao P, Li X, Xie K, Zhang J, Wang J, Liu F, Ma W, Shopan J, Zheng H, Mackenzie S A, et al. 2016. The genome sequence of allopolyploid Brassica juncea and analysis of differential homoeolog gene expression influencing selection. Nature Genetics, 48, 1225–1232.
Zhang D, Pan Q, Tan C, Liu L, Ge X, Li Z, Yan M. 2018. Homoeolog expression is modulated differently by different subgenomes in Brassica napus hybrids and allotetraploids. Plant Molecular Biology Reporter, 36, 387–398.
Zhang Y, Chen G, Dong T, Pan Y, Zhao Z, Tian S, Hu Z. 2014. Anthocyanin accumulation and transcriptional regulation of anthocyanin biosynthesis in purple bok choy (Brassica rapa var. chinensis). Journal of Agricultural and Food Chemistry, 62, 12366–12376.
Zhao Z, Xiao L, Xu L, Xing X, Tang G, Du D. 2017. Fine mapping the BjPl1 gene for purple leaf color in B2 of Brassica juncea L. through comparative mapping and whole-genome re-sequencing. Euphytica, 213, 80. |
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