|A conserved odorant receptor identified from antennal transcriptome of Megoura crassicauda that specifically responds to cis-jasmone
|WANG Bo1, 2*, HUANG Tian-yu2, 3, 4*, YAO Yuan1, Frederic FRANCIS4, YAN Chun-cai1, WANG Gui-rong2, 3, WANG Bing2
|1 Tianjin Key Laboratory of Conservation and Utilization of Animal Diversity, Tianjin Normal University, Tianjin 300387, P.R.China
2 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
3 Guangdong Laboratory for Lingnan Modern Agriculture (Shenzhen Branch), Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, P.R.China
4 Functional and Evolutionary Entomology, Gembloux Agro-Bio Tech, University of Liège, Gembloux 5030, Belgium
本研究首先对豌豆修尾蚜触角转录组进行测序，并对鉴定到的气味受体（odorant receptors, ORs）基因进行了表达水平分析。随后，为了研究豌豆修尾蚜识别HIPVs的化学感受机制，利用11种已鉴定的蚜虫为害诱导的HIPVs对ORs的体外功能进行了研究。研究结果表明，在豌豆修尾蚜触角转录组中共鉴定出54个化学感受基因。注释到20个ORs基因，与豌豆蚜ORs进行氨基酸相似性分析，发现McraOR20和McraOR43分别与豌豆蚜的同源受体序列具有较高的保守性，且在触角中的表达量较高。因此对McraOR20和McraOR43的体外功能进行了研究，结果显示豌豆修尾蚜McraOR20与豌豆蚜中的同源基因ApisOR20均能特异地识别一种HIPV顺式-茉莉酮，而McraOR43对测试的11种HIPVs均无电生理反应。本研究证实了两种蚜虫的同源受体OR20均能特异性识别虫害诱导的植物挥发物顺式-茉莉酮，为发展蚜虫的行为调控策略提供了候选的嗅觉受体靶标。
Abstract Herbivore-induced plant volatiles (HIPVs) play a key role in the interactions between plants and herbivorous insects, as HIPVs can promote or deter herbivorous insects’ behavior. While aphids are common and serious phloem-feeding pests in farmland ecosystems, little is known about how aphids use their sensitive olfactory system to detect HIPVs. In this study, the antennal transcriptomes of the aphid species Megoura crassicauda were sequenced, and expression level analyses of M. crassicauda odorant receptors (ORs) were carried out. To investigate the chemoreception mechanisms that M. crassicauda uses to detect HIPVs, we performed in vitro functional studies of the ORs using 11 HIPVs reported to be released by aphid-infested plants. In total, 54 candidate chemosensory genes were identified, among which 20 genes were ORs. McraOR20 and McraOR43 were selected for further functional characterization because their homologs in aphids were quite conserved and their expression levels in antennae of M. crassicauda were relatively high. The results showed that McraOR20 specifically detected cis-jasmone, as did its ortholog ApisOR20 from the pea aphid Acyrthosiphon pisum, while McraOR43 did not respond to any of the HIPV chemicals that were tested. This study characterized the ability of the homologous OR20 receptors in the two aphid species to detect HIPV cis-jasmone, and provides a candidate olfactory target for mediating aphid behaviors.
Received: 15 January 2021
Accepted: 16 April 2021
|Fund: This work was funded by the National Natural Science Foundation of China (31572072 and 31801994), the Shenzhen Science and Technology Program, China (KQTD20180411143628272), the Natural Science Foundation of Tianjin, China (18JCYBJC96100), and the Tianjin Normal University Foundation, China (135305JF79).
|About author: WANG Bo, E-mail: email@example.com; HUANG Tian-yu, E-mail: firstname.lastname@example.org; Correspondence YAN Chun-cai, E-mail: email@example.com; WANG Gui-rong, E-mail: firstname.lastname@example.org; WANG Bing, E-mail: email@example.com
* These authors contributed equally to this study.
Cite this article:
WANG Bo, HUANG Tian-yu, YAO Yuan, Frederic FRANCIS, YAN Chun-cai, WANG Gui-rong, WANG Bing.
A conserved odorant receptor identified from antennal transcriptome of Megoura crassicauda that specifically responds to cis-jasmone. Journal of Integrative Agriculture, 21(7): 2042-2054.
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