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Journal of Integrative Agriculture  2024, Vol. 23 Issue (3): 948-959    DOI: 10.1016/j.jia.2023.06.008
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Acaricidal effect of the antimicrobial metabolite xenocoumacin 1 on spider mite control

Jiaxing Wei1, Hong Yan1, Jie Ren1, 2, Guangyue Li1, 2#, Bo Zhang1, 3#, Xuenong Xu1, 3#

1 Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China

2 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Beijing 100193, China

3 Key Laboratory of Natural Enemies Insects, Ministry of Agriculture and Rural Affairs, Beijing 100193, China

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摘要  

二斑叶螨是全多种农业生态系统中普遍发生且影响极为严重有害生物之一,目前的主要防控手段是化学杀螨剂及捕食螨天敌,然而两者兼容性较低。为有效防治该虫害,迫切需要开发新型生物农药以支持二斑叶螨有害生物综合治理研究中,我们探明了高纯度抗菌代谢产物xenocoumacin 1Xcn1)对二斑叶螨及加州新小绥螨的致死效应,明确了该化合物对叶螨的防控作用及天敌友好程度。首先,我们利用简易启动子激活化合物识别方法(easyPACId)构建了嗜线虫致病杆菌CB6的突变体,在其无细胞上清液中提取并纯化获得Xcn1代谢产物。当喷施浓度超过100μg mL-1 的Xcn1溶液6二斑叶螨存活率降至不足40%繁殖率降低了80%此外,我们发现Xcn1在浓度为25μg mL-150μg mL-1时可通过抑制蜕皮中止二斑叶螨发育,但这两个浓度对捕食螨天敌加州新小绥螨的生存和繁殖没有任何不利影响。结合实验室和半田间实验结果,我们发现抗菌代谢Xcn1在分子水平种群水平上均具有控制害螨的可行性因此,本研究提供了结合Xcn1捕食螨类可兼容生物控制剂进行害螨综合防治的参考模式,为叶螨绿色防控技术提供了理论基础



Abstract  

The two-spotted spider mite, Tetranychus urticae Koch, is one of the most harmful pests in many agroecosystems worldwide.  To effectively manage this pest, there is an urgent need to develop novel bio-active acaricides that support integrated pest management strategies targeting Turticae.  In this study, we explored the acaricidal effects of xenocoumacin 1 (Xcn1) on Turticae and its predator Neoseiulus californicus using the highly purified compound.  Xcn1 was extracted and purified from the cell-free supernatant of the Xenorhabdus nematophila CB6 mutant constructed by the easy promoter activated compound identification (easyPACId) method.  When the concentration of Xcn1 exceeded 100 μg mL–1, the survival rate of spider mite adults declined to below 40% and the fecundity was decreased by 80% at six days post-application.  At concentrations of 25 and 50 μg mL–1, Xcn1 significantly impeded spider mite development by inhibiting the molt.  However, neither concentration had any adverse effects on the survival or reproduction of the predatory mite Ncalifornicus.  The results from laboratory and semi-field experiments consistently demonstrated the effectiveness of the antimicrobial metabolite Xcn1 in controlling pest mites at both the molecular and physiological levels.  Our study offers a promising possibility that combines the compatible biocontrol agents of Xcn1 and predatory mites for integrated pest mite control.

Keywords:  pest management        predatory mite        Xcn1        morphology        developmental inhibition        transcriptome   
Received: 24 February 2023   Accepted: 05 May 2023
Fund: 

This work was supported by the National Natural Science Foundation of China (32070402) and the Beijing Natural Science Foundation, China (6222052).

About author:  Jiaxing Wei, E-mail: weijiaxing7@163.com; #Correspondence Guangyue Li, E-mail: liguangyue@caas.cn; Bo Zhang, E-mail: zhangbo05@caas.cn; Xuenong Xu, E-mail: xuxuenong@caas.cn

Cite this article: 

Jiaxing Wei, Hong Yan, Jie Ren, Guangyue Li, Bo Zhang, Xuenong Xu. 2024.

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