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| Transcriptome analysis of the influence of CPPU application for fruit setting on melon volatile content |
| CHENG Jin-tao, CHEN Hai-wen, DING Xiao-chen, SHEN Tai, PENG Zhao-wen, KONG Qiu-sheng, HUANG Yuan, BIE Zhi-long |
| College of Horticulture and Forestry, Huazhong Agricultural University/Key Laboratory of Horticultural Plant Biology, Ministry of Education, Wuhan 430070, P.R.China |
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摘要
在果实生产过程中，植物生长调节剂CPPU的使用，往往引起果实香味变淡。本研究采用气相色谱-质谱连用分析仪和转录组测序来分析CPPU处理和授粉处理的甜瓜果实，以期探明CPPU影响甜瓜果实香味物质积累的原因。结果表明CPPU处理的甜瓜果实中，两个重要的酯类物质，乙酸苯甲酯和乙酸苯乙酯的含量显著低于蜜蜂授粉果实中的含量。转录组测序结果表明蜜蜂授粉和CPPU处理果实中的差异表达基因主要集中在苯丙氨酸代谢途径中。这些基因在CPPU处理果实中的表达量显著低于蜜蜂授粉果实。进一步分析发现CPPU处理的果实中苯丙氨酸的含量显著高于授粉果实中的含量。综合结果表明，CPPU可以干扰甜瓜果实中苯丙氨酸的代谢，进而影响芳香酯类物质的合成。
Abstract In fruit production, the application of the plant growth regulator 1-(2-chloro-4-pyridyl)-3-phenylurea (CPPU) dulls the fruit aroma. Gas chromatography–mass spectrometry and transcriptome analyses were performed on CPPU-treated and pollinated fruits to determine how CPPU affects the production of aroma in melon fruit. The results showed that the contents of two important esters (benzyl acetate and phenethyl acetate) in the CPPU-treated fruits were significantly lower than those in the pollinated fruits. Transcriptome sequencing data revealed that most differentially expressed genes were involved in “phenylalanine metabolism” pathway, and their expression was significantly decreased in the CPPU-treated fruits. Further analysis showed that the phenylalanine content in the CPPU-treated fruits was significantly higher than that in the pollinated fruits. In summary, CPPU application interferes with phenylalanine metabolism in melon fruits and affects the production of aromatic esters.
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Received: 18 May 2020
Accepted:
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| Fund: This work was supported by the China Agriculture Research System of MOF and MARA (CARS-25), the Special Fund for Agro-scientific Research in the Public Interest, China (201203080), the Fundamental Research Funds for the Central Universities, China (2662018PY039) and the Hubei Provincial Natural Science Foundation of China (2019CFA017). |
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Corresponding Authors:
Correspondence BIE Zhi-long, Tel: +86-27-87286908, Fax: +86-27-87282010, E-mail: biezl@mail.hzau.edu.cn
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| About author: CHENG Jin-tao, E-mail: chengjintao@mail.hzau.edu.cn; |
Cite this article:
CHENG Jin-tao, CHEN Hai-wen, DING Xiao-chen, SHEN Tai, PENG Zhao-wen, KONG Qiu-sheng, HUANG Yuan, BIE Zhi-long.
2021.
Transcriptome analysis of the influence of CPPU application for fruit setting on melon volatile content. Journal of Integrative Agriculture, 20(12): 3199-3208.
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