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Journal of Integrative Agriculture  2021, Vol. 20 Issue (12): 3199-3208    DOI: 10.1016/S2095-3119(21)63639-0
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Transcriptome analysis of the influence of CPPU application for fruit setting on melon volatile content
CHENG Jin-tao, CHEN Hai-wen, DING Xiao-chen, SHEN Tai, PENG Zhao-wen, KONG Qiu-sheng, HUANG Yuan, BIE Zhi-long
College of Horticulture and Forestry, Huazhong Agricultural University/Key Laboratory of Horticultural Plant Biology, Ministry of Education, Wuhan 430070, P.R.China
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In fruit production, the application of the plant growth regulator 1-(2-chloro-4-pyridyl)-3-phenylurea (CPPU) dulls the fruit aroma.  Gas chromatography–mass spectrometry and transcriptome analyses were performed on CPPU-treated and pollinated fruits to determine how CPPU affects the production of aroma in melon fruit.  The results showed that the contents of two important esters (benzyl acetate and phenethyl acetate) in the CPPU-treated fruits were significantly lower than those in the pollinated fruits.  Transcriptome sequencing data revealed that most differentially expressed genes were involved in “phenylalanine metabolism” pathway, and their expression was significantly decreased in the CPPU-treated fruits.  Further analysis showed that the phenylalanine content in the CPPU-treated fruits was significantly higher than that in the pollinated fruits.  In summary, CPPU application interferes with phenylalanine metabolism in melon fruits and affects the production of aromatic esters. 
Keywords:  aroma        volatiles        melon        CPPU        fruit set        transcriptome analysis        gas chromatography–mass spectrometry  
Received: 18 May 2020   Accepted:
Fund: This work was supported by the China Agriculture Research System of MOF and MARA (CARS-25), the Special Fund for Agro-scientific Research in the Public Interest, China (201203080), the Fundamental Research Funds for the Central Universities, China (2662018PY039) and the Hubei Provincial Natural Science Foundation of China (2019CFA017). 
Corresponding Authors:  Correspondence BIE Zhi-long, Tel: +86-27-87286908, Fax: +86-27-87282010, E-mail:   
About author:  CHENG Jin-tao, E-mail:;

Cite this article: 

CHENG Jin-tao, CHEN Hai-wen, DING Xiao-chen, SHEN Tai, PENG Zhao-wen, KONG Qiu-sheng, HUANG Yuan, BIE Zhi-long. 2021. Transcriptome analysis of the influence of CPPU application for fruit setting on melon volatile content. Journal of Integrative Agriculture, 20(12): 3199-3208.

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