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Journal of Integrative Agriculture  2021, Vol. 20 Issue (5): 1266-1276    DOI: 10.1016/S2095-3119(20)63415-3
Special Issue: Horticulture — Genetics · Breeding
Horticulture Advanced Online Publication | Current Issue | Archive | Adv Search |
Transcriptomic insights into growth promotion effect of Trichoderma afroharzianum TM2-4 microbial agent on tomato plants
ZHAO Juan, LIU Ting, LIU Wei-cheng, ZHANG Dian-peng, DONG Dan, WU Hui-ling, ZHANG Tao-tao, LIU De-wen
Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, P.R.China
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Abstract  
Plant growth promoting fungi are receiving increased attention as valuable beneficial microorganisms in crop cultivation due to their capacity to produce bioactive substances, promote plant growth and enhance immune defense functions.  In this study, a novel Trichoderma isolate, designated as TM2-4, was screened from healthy tomato rhizosphere soil and identified as Trichoderma afroharzianum.  Culture filtrate of the isolate TM2-4 displayed obvious bioactive substance production and an evident effect in promoting tomato seed germination, with hypocotyl length, radical length and vigor index increased by 28.7, 19.4 and 62.1%, respectively, after a 100-fold dilution treatment.  To assess the promotion effect and related mechanism of isolate TM2-4, the plant biological indexes and gene expression profiles of tomato plants treated with or without T. afroharzianum TM2-4 microbial agent were investigated by greenhouse pot experiment and RNA sequencing.  The results demonstrated that T. afroharzianum TM2-4 significantly promoted tomato plant growth in terms of plant height, dry weight, number of leaves per plant and root activity, through efficient colonization in the rhizosphere and root system of the plants.  Transcriptome analyses identified a total of 984 differentially expressed genes in T. afroharzianum microbial agent inoculated tomato roots, which were mainly engaged in the biological process of phytohormone homeostasis, antioxidant activity, as well as metabolic pathways including phenylpropanoid biosynthesis and glutathione metabolism.  These findings provide useful information for understanding the mechanism of isolate TM2-4 for tomato plant growth promotion, which would facilitate further development of T. afroharzianum TM2-4 microbial agent for use in vegetable crop production.
Keywords:  Trichoderma afroharzianum        Solanum lycopersicum L.        plant growth promotion        transcriptome analyses        qRT-PCR  
Received: 03 January 2020   Accepted: 12 April 2021
Fund: This research was supported by the Youth Research Fund of Beijing Academy of Agriculture and Forestry Sciences, China (QNJJ201814), the National Key R&D Program of China (2017YFD0201102), and the Beijing Key Laboratory of Green Control of Fruit Tree Diseases and Pests in the North China (BZ0432).
Corresponding Authors:  Correspondence LIU Ting, Tel/Fax: +86-10-51503337, E-mail: lting@163.com    
About author:  ZHAO Juan, E-mail: zhaojuan119882@163.com;

Cite this article: 

ZHAO Juan, LIU Ting, LIU Wei-cheng, ZHANG Dian-peng, DONG Dan, WU Hui-ling, ZHANG Tao-tao, LIU De-wen. 2021. Transcriptomic insights into growth promotion effect of Trichoderma afroharzianum TM2-4 microbial agent on tomato plants. Journal of Integrative Agriculture, 20(5): 1266-1276.

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