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Journal of Integrative Agriculture  2021, Vol. 20 Issue (9): 2424-2437    DOI: 10.1016/S2095-3119(20)63568-7
Special Issue: 园艺-分子生物合辑Horticulture — Genetics · Breeding
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Transcriptional profiles underlying the effects of salicylic acid on fruit ripening and senescence in pear (Pyrus pyrifolia Nakai)
SHI Hai-yan1*, CAO Li-wen2*, XU Yue1*, YANG Xiong1, LIU Shui-lin1, LIANG Zhong-shuo1, LI Guo-ce1, YANG Yu-peng1, ZHANG Yu-xing1, CHEN Liang2 
1 College of Horticulture, Hebei Agricultural University, Baoding 071001, P.R.China
2 Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, P.R.China
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本研究利用RNA测序技术,分析和比较了SA处理前后梨果实的转录表达谱。在SA处理12 h和24 h后,分别有159个和419个基因表达水平发生显著改变。在这些差异表达基因(Differentially expressed genes,DEGs)中,有125个基因在2个处理时间点均存在差异表达,被鉴定为可能与SA调控果实成熟衰老有关的候选基因。这些DEGs主要与植物激素的生物合成与代谢、细胞壁代谢与修饰、抗氧化系统和衰老相关转录因子等有关。此外,通过实时定量PCR(qRT-PCR)进一步验证了SA处理后梨果实中几种候选DEGs的表达。本研究有助于加深对SA调控梨果实成熟衰老综合机制的认识。

Salicylic acid (SA) plays a pivotal role in delaying fruit ripening and senescence.  However, little is known about its underlying mechanism of action.  In this study, RNA sequencing was conducted to analyze and compare the transcriptome profiles of SA-treated and control pear fruits.  We found a total of 159 and 419 genes differentially expressed between the SA-treated and control pear fruits after 12 and 24 h of treatment, respectively.  Among these differentially expressed genes (DEGs), 125 genes were continuously differentially expressed at both treatment times, and they were identified as candidate genes that might be associated with SA-regulated fruit ripening and senescence.  Bioinformatics analysis results showed that 125 DEGs were mainly associated with plant hormone biosynthesis and metabolism, cell wall metabolism and modification, antioxidant systems, and senescence-associated transcription factors.  Additionally, the expression of several candidate DEGs in ripening and senescent pear fruits after SA treatments were further validated by quantitative real-time PCR (qRT-PCR).  This study provides valuable information and enhances the understanding of the comprehensive mechanisms of SA-meditated pear fruit ripening and senescence.
Keywords:  pear        salicylic acid        fruit ripening and senescence        transcriptome        DEGs  
Received: 15 June 2020   Accepted:
Fund: This work was supported by the National Natural Science Foundation of China (31301761), the China Scholarship Council (201608130248), and the Second Round of the Youth Top-Notch Talent Support Programs of Hebei Province, China (2019).
Corresponding Authors:  Correspondence SHI Hai-yan, Tel/Fax: +86-312-7528300, E-mail:; CHEN Liang, E-mail:   
About author:  * These authors contributed equally to this study.

Cite this article: 

SHI Hai-yan, CAO Li-wen, XU Yue, YANG Xiong, LIU Shui-lin, LIANG Zhong-shuo, LI Guo-ce, YANG Yu-peng, ZHANG Yu-xing, CHEN Liang. 2021. Transcriptional profiles underlying the effects of salicylic acid on fruit ripening and senescence in pear (Pyrus pyrifolia Nakai). Journal of Integrative Agriculture, 20(9): 2424-2437.

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