Integration of the metabolome and transcriptome reveals the metabolites and genes related to nutritional and medicinal value in Coriandrum sativum
WU Tong1, FENG Shu-yan1, YANG Qi-hang1, Preetida J BHETARIYA2, GONG Ke1, CUI Chun-lin1, SONG Jie1, PING Xiao-rui1, PEI Qiao-ying1, YU Tong1, SONG Xiao-ming1, 3, 4
1 College of Life Sciences, North China University of Science and Technology, Tangshan 063210, P.R.China 2 Department of Biostatistics, Harvard School of Public Health, Boston, MA 02115, USA 3 Food Science and Technology Department, University of Nebraska-Lincoln, Lincoln, NE 68526, USA 4 Center for Informational Biology, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, P.R.China
Coriandrum sativum (Coriander) or Chinese parsley is a culinary herb with multiple medicinal effects, which is widely used in cooking and traditional medicine. It is enriched with essential oils and anti-oxidant compounds with unknown significance. To explore the untapped reservoir of Coriander, we studied the transcriptome and metabolic profiles from three developmental stages. Here, we identified 10 tyrosine metabolic pathway-related genes (TMPRGs), six porphyrins and chlorophyll metabolic pathway-related genes (PCMPRGs), and five Vitamin E metabolic pathway-related genes (VEMPRGs). These genes were associated with the early development of Coriander. Our analysis suggests that these pathways are involved in the production of critical phenolic metabolites. Furthermore, we constructed the interaction network between these pathway-related genes and transcription factors (TFs), which supported the regulatory pathways for phenolic metabolites. Interestingly, we identified several nutritional or medicinally relevant metabolites, including 59 phenols, two polyamines, 12 alkaloids, and one terpenoid. The higher concentrations of metabolites were from caffeic acid, agmatine, and its derivatives. We found higher levels of caffeic acid and agmatine at 30 days compared to 60 or 90 days. This study provides evidence to stimulate further investigation of the role of these metabolites in medicinal and nutritional research.
Fund: This work was supported by the National Natural Science Foundation of China (31801856), the Hebei Province Higher Education Youth Talents Program, China (BJ2018016), and the Hebei Province Postgraduate Demonstration Course (Genomics) Construction Project in 2018, China (KCJSX2018053).
Corresponding Authors:
Correspondence SONG Xiao-ming, Tel/Fax: +86-315-8805607, E-mail: songxm@ncst.edu.cn
WU Tong, FENG Shu-yan, YANG Qi-hang, Preetida J BHETARIYA, GONG Ke, CUI Chun-lin, SONG Jie, PING Xiao-rui, PEI Qiao-ying, YU Tong, SONG Xiao-ming.
2021.
Integration of the metabolome and transcriptome reveals the metabolites and genes related to nutritional and medicinal value in Coriandrum sativum. Journal of Integrative Agriculture, 20(7): 1807-1818.
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