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Journal of Integrative Agriculture  2016, Vol. 15 Issue (4): 735-743    DOI: 10.1016/S2095-3119(15)61299-0
Crop Genetics · Breeding · Germplasm Resources Advanced Online Publication | Current Issue | Archive | Adv Search |
Mapping of three QTLs for seed setting and analysis on the candidate gene for qSS-1 in rice (Oryza sativa L.)
Elsheikh Y M Ahmed, ZHANG Yan-pei, YU Jian-ping, Rashid M A Rehman, ZHANG Zhan-ying, ZHANG Hong-liang, LI Jin-jie, LI Zi-chao
Key Laboratory of Crop Heterosis and Utilization, Ministry of Education/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, P.R.China
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摘要  The lower seed setting is one of the major hindrances which face grain yield in rice. One of the main reasons to cause low spikelet fertility (seed setting) is male sterility or pollen abortion. Notably, pollen abortion has been frequently observed in advanced progenies of rice. In the present study, 149 BC2F6 individuals with significant segregation in spikelet fertility were generated from the cross between N040212 (indica) and Nipponbare (japonica) and used for primary gene mapping. Three QTLs, qSS-1, qSS-7 and qSS-9 at chromosomes 1, 7 and 9, respectively, were found to be associated with seed setting. The recombinant analysis and the physical mapping information from publicly available resources exhibited that the qSS-1, qSS-7 and qSS-9 loci were mapped to an interval of 188, 701 and 3 741 kb, respectively. The seed setting responsible for QTL qSS-1 was further fine mapped to 93.5 kb by using BC2F7 population of 1 849 individuals. There are 16 possible putative genes in this 93.5 kb region. Pollen vitality tests and artificial pollination indicated that the male gamete has abnormal pollen while the female gamete was normal. These data showed that low seed setting rate relative to qSS-1 may be caused by abnormal pollen grains. These results will be useful for cloning, functional analysis of the target gene governing spikelet fertility (seed setting) and understanding the genetic bases of pollen sterility.

Abstract  The lower seed setting is one of the major hindrances which face grain yield in rice. One of the main reasons to cause low spikelet fertility (seed setting) is male sterility or pollen abortion. Notably, pollen abortion has been frequently observed in advanced progenies of rice. In the present study, 149 BC2F6 individuals with significant segregation in spikelet fertility were generated from the cross between N040212 (indica) and Nipponbare (japonica) and used for primary gene mapping. Three QTLs, qSS-1, qSS-7 and qSS-9 at chromosomes 1, 7 and 9, respectively, were found to be associated with seed setting. The recombinant analysis and the physical mapping information from publicly available resources exhibited that the qSS-1, qSS-7 and qSS-9 loci were mapped to an interval of 188, 701 and 3 741 kb, respectively. The seed setting responsible for QTL qSS-1 was further fine mapped to 93.5 kb by using BC2F7 population of 1 849 individuals. There are 16 possible putative genes in this 93.5 kb region. Pollen vitality tests and artificial pollination indicated that the male gamete has abnormal pollen while the female gamete was normal. These data showed that low seed setting rate relative to qSS-1 may be caused by abnormal pollen grains. These results will be useful for cloning, functional analysis of the target gene governing spikelet fertility (seed setting) and understanding the genetic bases of pollen sterility.
Keywords:  rice (Oryza sativa L.)       QTL mapping       seed setting       pollen sterility  
Received: 07 April 2015   Accepted:
Fund: 

This study was supported by the National Key Technologies R&D Program of China during the 12th Five-Year Plan period (2013BAD01B02-15 and 2015BAD02B01), and the 948 Project of Minstry of Agriculture, China (2011-G2B and 2011-G1 (2)-25).

Corresponding Authors:  LI Zi-chao, Tel: +86-10-62731414, E-mail: lizichao@cau.edu.cn     E-mail:  lizichao@cau.edu.cn

Cite this article: 

Elsheikh Y M Ahmed, ZHANG Yan-pei, YU Jian-ping, Rashid M A Rehman, ZHANG Zhan-ying, ZHANG Hong-liang, LI Jin-jie, LI Zi-chao. 2016. Mapping of three QTLs for seed setting and analysis on the candidate gene for qSS-1 in rice (Oryza sativa L.). Journal of Integrative Agriculture, 15(4): 735-743.

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