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Special Issue:
麦类遗传育种合辑Triticeae Crops Genetics · Breeding · Germplasm Resources
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| Identification of QTL for adult plant resistance to stripe rust in bread wheat line C33 |
LUO Jiang-tao*, ZHENG Jian-min*, WAN Hong-shen, YANG Wu-yun, LI Shi-zhao, PU Zong-jun |
Crop Research Institute, Sichuan Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Breeding in Wheat (Southwest), Ministry of Agriculture and Rural Affairs, Chengdu 610066, P.R.China |
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Abstract Stripe rust, caused by Puccinia striiformis f. sp. tritici, is a serious disease in bread wheat (Triticum aestivum L.). Identification and use of adult plant resistance (APR) resources are important for stripe rust resistance breeding. Bread wheat line C33 is an exotic germplasm that has shown stable APR to stripe rust for more than 10 years in Sichuan Province of China. Here, 183 recombinant inbred lines (RILs) derived from the cross between C33 and a susceptible line X440 were genotyped with diversity arrays technology (DArT) markers to identify resistance quantitative trait locus (QTL). Field trials were conducted in five years at Chengdu and Xindu of Sichuan Province, using maximum disease severity (MDS) as stripe rust reaction phenotypes. A total of four quantitative trait loci (QTLs) were detected, respectively designed as QYr.saas-3AS, QYr.saas-5AL, QYr.saas-5BL, and QYr.saas-7DS, explaining 4.14–15.21% of the phenotypic variances. QYr.saas-5BL and QYr.saas-7DS were contributed by C33. However, the level for stripe rust resistance contributed by them was not strong as C33, suggesting the presence of other unidentified QTLs in C33. QYr.saas-7DS corresponded to Yr18 and QYr.saas-5BL remains to be formally named. The RIL lines carrying combinations QYr.saas-5AL, QYr.saas-5BL, and QYr.saas-7DS showed comparability resistance with C33. The present study provides resources to pyramid diverse genes into locally adapted elite germplasm to improve the stripe rust resistance of bread wheat.
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Received: 20 November 2018
Accepted:
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| Fund: The authors thanks to International Center for Agricultural Research in the Dry Areas, for providing the bread wheat line C33. This research was supported by the National Natural Science Foundation of China (31671683) and the Youth Foundation of Sichuan Academy of Agricultural Sciences, China (2016QNJJ-008). |
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Corresponding Authors:
Correspondence PU Zong-jun, +86-28-84504240, E-mail: pzjun68@163.com
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| About author: LUO Jiang-tao, +86-28-84504260, E-mail: jtluohao@163.com; * These authors contributed equally to this study. |
Cite this article:
LUO Jiang-tao, ZHENG Jian-min, WAN Hong-shen, YANG Wu-yun, LI Shi-zhao, PU Zong-jun .
2020.
Identification of QTL for adult plant resistance to stripe rust in bread wheat line C33. Journal of Integrative Agriculture, 19(3): 624-631.
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Bariana H S, Bansal U K, Schmidt A, Lehmensiek A, Kaur J, Miah H, Howea N, McIntyre C L. 2010. Molecular mapping of adult plant stripe rust resistance in wheat and identification of pyramided QTL genotypes. Euphytica, 176, 251–260.
Chen W Q, Wu L R, Liu T G, Xu S C, Jin S L, Peng Y L, Wang B T. 2009. Race dynamics, diversity, and virulence evolution in Puccinia striiformis f. sp. tritici, the causal agent of wheat stripe rust in China from 2003 to 2007. Plant Disease, 93, 1093–1101.
Chen X M. 2013. High-temperature adult-plant resistance, key for sustainable control of stripe rust. American Journal of Plant Sciences, 4, 608–627.
Chen X M, Kang Z S. 2017. Stripe Rust. Springer Science+Bussiness Media B. V., Berlin, Germany.
Chhuneja P, Kaur S, Garg T, Ghai M, Kaur S, Prashar M, Bains N S, Goel R K, Keller B, Dhaliwal H S, Singh K. 2008. Mapping of adult plant stripe rust resistance genes in diploid A genome wheat species and their transfer to bread wheat. Theoretical and Applied Genetics, 116, 313–324.
Doerge R W, Churchill G A. 1996. Permutation tests for multiple loci affecting a quantitative character. Genetics, 142, 285–294.
Dong Z Z, Hegarty J M, Zhang J L, Zhang W J, Chao S M, Chen X M, Zhou Y H, Dubcovsky J. 2017. Validation and characterization of a QTL for adult plant resistance to stripe rust on wheat chromosome arm 6BS (Yr78). Theoretical and Applied Genetics, 130, 2127–2137.
Dracatos P M, Zhang P, Park R F, McIntosh R A, Wellings C R. 2016. Complementary resistance genes in wheat selection ‘Avocet R’ confer resistance to stripe rust. Theoretical and Applied Genetics, 129, 65–76.
Fang T L, Campbell K G, Liu Z Y, Chen X M, Wan A M, Li S, Liu Z J, Cao S H, Chen Y H, Bowden R L, Carver B F, Yan L L. 2011. Stripe rust resistance in the wheat cultivar Jagger is due to Yr17 and a novel resistance gene. Crop Science, 51, 2455–2465.
Feng J Y, Wang M L, See D R, Chao S M, Zheng Y L, Chen X M. 2018. Charaterization of novel gene Yr79 and four additional quantitative trait loci for all-stage and high-temperature adult-plant resistance to stripe rust in spring wheat PI182103. Phytopathology, 108, 737–747.
Francki M G, Walker E, Crawford A C, Broughton S, Ohm H W, Barclay I, Wilson R E. 2009. Comparison of genetic and cytogenetic maps of hexaploid wheat (Triticum aestivum L.) using SSR and DArT markers. Molecular Genetices and Genomics, 281, 181–191.
Fu D L, Uauy C, Distelfeld A, Blechl A, Epstein L, Chen X M, Sela H, Fahima T, Dubcovsky J. 2009. A novel kinase-START gene confers temperature-dependent resistance to wheat stripe rust. Science, 323, 1357–1360.
IWGSC (International Wheat Genome Sequencing Consortium). 2018. Shifting the limits in wheat research and breeding using a fully annotated reference genome. Science, 361, eaar7191.
Jighly A, Oyiga B C, Makdis F, Nazari K, Youssef O, Tadesse W, Abdalla O, Ogbonnaya F C. 2015. Genomewide DArT and SNP scan for QTL associated with resistance to stripe rust (Puccinia striiformis f. sp. tritici) in elite ICARDA wheat (Triticum aestivum L.) germplasm. Theoretical and Applied Genetics, 128, 1277–1295.
Krattinger S G, Lagudah E S, Spielmeyer W, Singh R P, Huerta-Espino J, McFadden H, Bossolini E, Selter L L, Keller B. 2009. A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat. Science, 323, 1360–1363.
Lan C X, Liang S S, Zhou X C, Zhou G, Lu Q L, Xia X C, He Z H. 2010. Identification of genomic regions controlling adult-plant stripe rust resistance in Chinese landrace Pingyuan 50 through bulked segregant analysis. Phytopathology, 100, 313–318.
Lillemo M, Asalf B, Singh R P, Huerta-Espino J, Chen X M, He Z H, Bjørnstad A. 2008. The adult plant rust resistance loci Lr34/Yr18 and Lr46/Yr29 are important determinants of partial resistance to powdery mildew in bread wheat line Saar. Theoretical and Applied Genetics, 116, 1155–1166.
Lowe I, Jankuloski L, Chao S, Chen X M, See D, Dubcovsky J. 2011. Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat. Theoretical and Applied Genetics, 123, 143–157.
Lu Y M, Lan C X, Liang S S, Zhou X C, Liu D, Zhou G, Lu Q L, Jing J X, Wan M L, Xia X C, He Z H. 2009. QTL mapping for adult-plant resistance to stripe rust in Italian common wheat cultivars Libellula and Strampelli. Theoretical and Applied Genetics, 119, 1349–1359.
Malihipour A, Torabi M, Ahmadian-Moghaddam M S, Tarinejad A. 2012. Reaction of some international wheat genotypes to yellow rust at the adult-plant stage in Iran. In: Yahyaoui A, Rajaram S, eds., Meeting the Challenge of Yellow Rust in Cereal Crops. International Center for Agricultural Research in the Dry Areas Press, Syria. pp. 117–126.
McIntosh R A, Wellings C R, Park R F. 1995. Wheat Rusts: An Atlas of Resistance Genes. Commonwealth Scientific and Industrial Research Organization (CSIRO), Australia. pp. 1–200.
Mclntosh R A, Yamazaki Y, Dubcovsky J, Rogers J, Morris C, Appels R, Xia X C. 2013. Catalogue of gene symbols for wheat. In: 12th International Wheat Genetic Symposium, 8–13 September, Japan.
Meng L, Li H H, Zhang L Y, Wang J K. 2015. QTL IciMapping: Integrated software for genetic linkage map construction and quantitative trait locus mapping in biparental populations. The Crop Journal, 3, 269–283.
Peterson R F, Campbell A B, Hannah A E. 1948. A diagrammatic scale for estimating rust intensity on leaves and stems of cereals. Canadian Journal of Research, 26, 496–500.
Ramburan V P, Pretorius Z A, Louw J H, Boyd L A, Smith P H, Boshoff W H P, Prins R. 2004. A genetic analysis of adult plant resistance to stripe rust in the wheat cultivar Kariega. Theoretical and Applied Genetics, 108, 1426–1433.
Ren Y, He Z H, Li J, Lillemo M, Wu L, Bai B, Lu Q X, Zhu H Z, Zhou G, Du J Y, Lu Q L, Xia X C. 2012. QTL mapping of adult-plant resistance to stripe rust in a population derived from common wheat cultivars Naxos and Shanghai 3/Catbird. Theoretical and Applied Genetics, 125, 1211–1221.
Rosewarne G M, Herrera-Foessel S A, Singh R P, Huerta-Espino J, Lan C X, He Z H. 2013. Quantitative trait loci of stripe rust resistance in wheat. Theoretical and Applied Genetics, 126, 2427–2449.
Rosewarne G M, Singh R P, Huerta-Espino J, Herrera-Foessel S A, Forrest K L, Hayden M J, Rebetzke G J. 2012. Analysis of leaf and stripe rust severities reveals pathotype changes and multiple minor QTL associated with resistance in an Avocet 3 Pastor wheat population. Theoretical and Applied Genetics, 124, 1283–1294.
Singh R P, Huerta-Espino J, Bhavani S, Herrera-Foessel S A, Singh D, Singh P K, Velu G, Mason R E, Njau P, Crossa J. 2011. Race non-specific resistance to rust diseases in CIMMYT spring wheats. Euphytica, 179, 175–186.
Suenaga K, Singh R P, Huerta-Espino J, William H M. 2003. Microsatellite markers for genes Lr34/Yr18 and other quantitative trait loci for leaf rust and stripe rust resistance in bread wheat. Phytopathology, 93, 881–890.
Wan A M, Zhao Z H, Chen X M, He Z H, Jin S L, Jia Q Z, Yao G, Yang J X, Wang B T, Li G B, Bi Y Q, Yan Z Y. 2004. Wheat stripe rust epidemic and virulence of Puccinia striiformis f. sp. tritici in China in 2002. Plant Disease, 88, 896–904.
Wellings C R. 2011. Giobal status of stripe rust: A review of historical and current threats. Euphytica, 179, 129–141.
Xiang C, Feng J Y, Wang M N, Chen X M, See D R, Wan A M, Wang T. 2016. Molecular mapping of stripe rust resistance gene Yr76 in winter club wheat cultivar Tyee. Phytopathology, 106, 1186–1193.
Yang E N, Rosewarne G M, Herrera-Foessel S A, Huerta-Espino J, Tang Z X, Fu S L, Ren Z L, Singh R P. 2013. QTL analysis of the spring wheat ‘‘Chapio’’ identifies stable stripe rust resistance despite inter-continental genotype×environment interactions. Theoretical and Applied Genetics, 126, 1721–1732.
Zeng Q D, Han D J, Wang Q L, Yuan F P, Wu J H, Zhang L, Wang X J, Huang L L, Chen X M, Kang Z S. 2014. Stripe rust resistance and genes in Chinese wheat cultivars and breeding lines. Euphytica, 196, 271–284.
Zwart R S, Thompson J P, Milgate A W, Bansal U K, Williamson P M, Raman H, Bariana H S. 2010. QTL mapping of multiple foliar disease and root-lesion nematode resistances in wheat. Molecular Breeding, 26, 107–124.
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