不同锌水平下GFabV对‘阳光玫瑰’葡萄光合效率及光合相关基因表达的影响

doi:10.3864/j.issn.0578-1752.2025.24.007

Abstract

Abstract:

【Objective】This study aimed to explore the effects of GFabV on photosynthetic characteristics in ‘Shine Muscat’ grapevines under different Zn levels, and to elucidate the molecular mechanism of GFabV affecting leaf photosynthetic efficiency by analyzing the relative expression differences of photosynthesis-related genes.【Method】‘Shine Muscat’ grapevines infected with GFabV were treated with Zn-sufficient (0.22 mg·L^-1) and Zn-deficient (0) Hoagland nutrient solution, respectively, and virus-free plants were used as control. The symptom of grapevine leaves was observed, and Zn content, GFabV expression level, photosynthetic parameters, chlorophyll fluorescence parameters, and chlorophyll content in the leaves were measured. Differentially expressed genes (DEGs) related to photosynthesis were screened, and expression level of key genes was verified.【Result】Under Zn deficiency, the Zn contents in the leaves of plants infected with GFabV and virus-free plants were 1.79 and 9.59 mg·kg^-1, respectively, and the Zn contents of those under Zn sufficiency were 18.84 and 23.16 mg·kg^-1, respectively. The leaves of plants infected with GFabV showed shrinkage leaflets, and slight chlorosis symptoms under Zn deficiency, and GFabV relative expression was significantly higher than that of the leaves infected with GFabV under Zn sufficiency. The leaves of plants infected with GFabV did not show symptoms under Zn sufficiency. The leaves of virus-free plants showed slight chlorotic symptoms under Zn deficiency. In comparison to the virus-free plants treated with Zn sufficiency, the contents of Chla, Chlb, Chla+b, Fv/Fm and ΦPSII in leaves of the plants infected with GFabV and the virus-free plants under Zn deficiency were significantly decreased. The chlorophyll (a, b, a+b) contents, Fv/Fm and ΦPSII in the leaves of the plants infected with GFabV under Zn deficiency were the lowest, which were 1.22, 0.35, 1.57 mg·g^-1, 0.40 and 0.04, respectively. The net photosynthetic rate (Pn), transpiration rate (Tr), and stomatal conductance (Gs) of the plants infected with GFabV and the virus-free plants under Zn deficiency were significantly lower than those of the virus-free plants under Zn sufficiency. The Pn, Tr, and Gs in the leaves of plants infected with GFabV under Zn deficiency decreased to 16.27%, 20.08% and 21.99% of that in the leaves of the virus-free plants under Zn sufficiency, respectively. Transcriptome sequencing and RT-qPCR results showed that the expression levels of PsbP, petC, PSBO, and psaD in the leaves infected with GFabV under Zn deficiency decreased to 41.50%, 54.81%, 41.04% and 44.45%, respectively, compared with those of the virus-free plants under Zn sufficiency. The expression levels of ATPD, SEND33, and psbA in the leaves were significantly decreased under Zn sufficiency. The expression levels of PPD4 and psbA in the leaves of virus-free plants were significantly decreased under Zn deficiency. The expression levels of PsbP, PSBO, and psaD in leaves infected with GFabV under Zn deficiency decreased to 55.03%, 43.26% and 53.36%, respectively, compared with those of the leaves infected with GFabV under Zn sufficiency.【Conclusion】The chlorophyll content of ‘Shine Muscat’ grapevine leaves carrying GFabV was significantly reduced, and the expression level of GFabV was increased under Zn deficiency. The leaves showed shrinkage leaflets and slight chlorosis symptoms. GFabV down-regulated photosynthesis-related genes expression such as PsbP, PSBO, and psaD, resulting in a significant decrease in photosynthetic parameters and chlorophyll fluorescence parameters in leaves, which affected the photosynthetic efficiency of leaves.

Key words: ‘Shine Muscat’ grapevine, grapevine fabavirus (GFabV), Zn deficiency, gene expression level, photosynthetic characteristics, photosynthesis-related gene

ZHANG XiangKun, LI JiaYing, QIAO RuMeng, HE JingLei, WANG Li, SHI XiaoXin, DU GuoQiang. Effects of GFabV Under Different Zn Levels on Photosynthetic Efficiency and Photosynthesis-Related Gene Expression of ‘Shine Muscat’ Grapevine[J].Scientia Agricultura Sinica, 2025, 58(24): 5190-5200.

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化合物 Compound	含量Content (mg·L^-1)
化合物 Compound	锌充足 Zn sufficiency	锌缺乏 Zn deficiency
Ca(NO₃)₂·4H₂O	945	945
KNO₃	607	607
NH₄H₂PO₄	115	115
MgSO₄·7H₂O	493	493
乙二胺四乙酸二钠铁NaFe-EDTA	40	40
硼酸H₃BO₃	2.86	2.86
MnSO₄·4H₂O	2.13	2.13
ZnSO₄·7H₂O	0.22	0
CuSO₄·5H₂O	0.08	0.08
钼酸铵(NH₄)₂Mo₇O₂₄·4H₂O	0.025	0.025

基因名称 Gene name	引物序列 Primer sequence (5′-3′)	产物大小 Product size (bp)
petC	F: GCCACCTATGGTATTAACGCGG R: GTCCTCTGACAACCCTGCCT	130
PSBO	F: TCGCTTGACCTACACCCTCG R: CCCGATACGAGGGGACCAAG	218
ATPD	F: AACGCAACGTTTTGGACGAG R: TGCTCCGACTCCAGTTTCAC	196
psaD	F: TGAGTTGGACCCCAATACGC R: GCACTGCTCTTTCCTAGCCA	196
PsbP	F: TTTCCAGTGCAGACAGCGTG R: TGTCTCGGGTCCATTCTGGT	174
SEND33	F: GTGAGCACCTCCTTCATCCG R: CCAAACAGAGCCTGACCCAC	77

处理 Treatment	叶绿素a含量 Chla content (mg·g^-1)	叶绿素b含量 Chlb content (mg·g^-1)	总叶绿素含量 Chl a+b content (mg·g^-1)	PSII最大光化学效率 Fv/Fm	PSII实际光化学效率 ΦPSII
GFabV+/Zn-	1.22±0.07c	0.35±0.06c	1.57±0.12c	0.40±0.02c	0.04±0.01c
GFabV+/Zn+	1.64±0.03b	0.64±0.01b	2.28±0.04b	0.58±0.08b	0.08±0.02b
GFabV-/Zn-	1.59±0.02b	0.63±0.03b	2.21±0.03b	0.60±0.09b	0.07±0.01b
GFabV-/Zn+	2.00±0.04a	0.80±0.05a	2.80±0.09a	0.75±0.03a	0.14±0.03a

基因ID Gene ID	基因名称 Gene name	Log₂FC
基因ID Gene ID	基因名称 Gene name	GFabV+/Zn- vs GFabV-/Zn+	GFabV-/Zn- vs GFabV-/Zn+	GFabV+/Zn+ vs GFabV-/Zn+	GFabV+/Zn- vs GFabV+/Zn+
LOC100252244	PPD4	-1.10	-0.64	-0.98	—
LOC132254568	psbA	-0.87	-0.87	-1.04	—
LOC100258958	ATPC	2.37	2.07	1.48	—
LOC100260811	PSBO	-1.09	—	—	-0.79
LOC100242600	psaD	-1.41	—	—	-1.08
LOC100261871	PsbP	-1.00	—	—	-0.61
LOC100245219	petC	-1.03	—	-0.66	—
LOC100251062	ATPD	-1.04	—	-0.56	—
LOC100249080	SEND33	-1.03	—	-0.74	—
LOC104878430	psbH	-3.64	—	—	-3.30

Effects of GFabV Under Different Zn Levels on Photosynthetic Efficiency and Photosynthesis-Related Gene Expression of ‘Shine Muscat’ Grapevine

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