Scientia Agricultura Sinica ›› 2025, Vol. 58 ›› Issue (20): 4178-4188.doi: 10.3864/j.issn.0578-1752.2025.20.011

• ECOLOGICAL UTILIZATION OF SALINE-ALKALI LAND • Previous Articles     Next Articles

The Effects of Biochar Combined with Fulvic Acid on the Physical and Chemical Properties, Enzyme Activities and Multifunctionality of Soil in Coastal Saline-Alkali Land

ZHANG HaiRui1,2(), JIA AngYuan1, GAO QiQi1, HAN ZheQun1, NAN ShanShan1, DUAN BiHua2(), WU XuePing1,3()   

  1. 1 State Key Laboratory of Efficient Utilization of Arid and Semi-Arid Arable Land in Northern China (Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences), Beijing 100081
    2 College of Biology and Resources Environment, Beijing University of Agriculture/Key Laboratory of North China Urban Agriculture, Ministry of Agriculture and Rural Affairs, Beijing 102206
    3 National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Dongying 257347, Shandong
  • Received:2025-08-05 Accepted:2025-09-24 Online:2025-10-16 Published:2025-10-14
  • Contact: DUAN BiHua, WU XuePing

Abstract:

【Objective】This study aimed to explore the effects of biochar combined with fulvic acid on the improvement of coastal saline-alkali soil and its influence on soil multifunctionality (SMF), providing the theoretical support for the scientific application of biochar and fulvic acid.【Method】Based on a three-year field experimental study, four treatments were set up in the coastal saline-alkali land, including (control (CK), single application of 30 t·hm-2 biochar (C1), single application of 0.15 t·hm-2 fulvic acid (H1), and combined application of biochar and fulvic acid (C1H1). The soil physical and chemical properties and soil enzyme activities in the 0-10 cm, 10-20 cm soil layers and rhizosphere soil were determined, and the soil multifunctionality was calculated based on the average value method.【Result】The results showed that the combined application of biochar and fulvic acid could significantly improve the physical, chemical and biological properties of soil. Compared with CK, the soil bulk density under C1H1 treatment at 0-10 cm and 10-20 cm soil layers decreased by 9.2% and 11.2%, respectively; the saturated soil moisture content increased by 11.3% and 9.8%, respectively; the total soil porosity increased by 12.6% and 12.8%, respectively; the soil pH value decreased by 4.4% and 3.9%, respectively; the soil EC value decreased by 19.9% and 22.8%, respectively; the soil SOC increased by 44.6% and 44.7%, respectively; available phosphorus (AP) increased by 48.3% and 44.8%, respectively; the availability of potassium (AK) increased by 45.3% and 43.3%, respectively. The enzyme activity analysis revealed that C1H1 had the most significant promoting effect on the carbon-nitrogen cycle-related enzymes (β-glucosidase, cellobiose hydrolase, leucine aminopeptidase), with an increase ranging from 23.4% to 47%. Under the combined treatment of biochar and fulvic acid, the SMF index had the greatest increase, rising by 77.5%. 【Conclusion】The combined application of biochar and fulvic acid significantly enhanced soil multifunctionality by improving the pore structure of coastal saline-alkali land and increasing nutrient availability and enzyme activity.

Key words: biochar, fulvic acid, physical and chemical properties of soil, soil enzyme activity, soil multifunctionality

Table 1

The basic properties of biochar and fulvic acid"

原料
Raw material
pH C
(%)
N
(%)
比表面积
Specific surface area (m2·g-1)
容重
Bulk density (g·cm-3)
水溶性
Water solubility (%)
氨基酸
Amino acid
(%)
生物炭 Biochar 花生壳 Peanut shell 8.33 52.41 2.31 16.72 0.22
黄腐酸Fulvic acid 秸秆 Straw 7.00 99 5

Table 2

Basic physical and chemical properties of coastal saline-alkali land soil"

土层
Soil layer (cm)
速效钾
Available potassium (mg·kg-1)
有机碳
Soil organic carbon (g·kg-1)
pH 电导率
Electric conductivity (µS·cm-1)
土壤容重
Bulk density
(g·cm-3)
砂粒
Sand
(%)
粉粒
Silt
(%)
黏粒
Clay
(%)
0-20 90.10 7.81 8.93 1716 1.55 16.97 61.30 21.73
20-40 89.36 7.69 8.86 1714 1.54 15.33 71.73 18.93

Fig. 1

The effects of different treatments on bulk density, soil moisture content and soil porosity CK, C1, H1, and C1H1 represent the control, 30 t·hm-2 biochar, 0.15 t·hm-2 fulvic acid, and 30 t·hm-2 biochar +0.15 t·hm-2 fulvic acid, respectively; Different lowercase letters indicate significant differences between treatments (P<0.05); The error line represents the standard error. The same as below"

Fig. 2

The effects of different treatments on pH and Electric conductivity"

Fig. 3

The effects of different treatments on SOC, AP and AK"

Table 3

Enzyme activities under different treatments"

处理
Treatment
BG
(mmol·g-1·h-1)
CBH
(mmol·g-1·h-1)
NAG
(mmol·g-1·h-1)
LAP
(mmol·g-1·h-1)
ACP
(mmol·g-1·h-1)
CK 1.95±0.03b 1.02±0.06d 0.43±0.04c 82.6±4.72b 5.47±0.25c
C1 2.76±0.2a 1.5±0.12b 0.59±0.05b 101.96±6.94a 6.15±0.31bc
H1 2±0.16b 1.22±0.12c 0.45±0.03c 84.13±4.71b 6.45±0.33b
C1H1 3.09±0.25a 1.75±0.04a 0.89±0.08a 107.18±5.79a 9.82±0.73a

Fig. 4

The influence of different treatments on soil multifunctionality index(SMFI) (a) and the analysis of soil physical and chemical properties, SMF functional indicators, and SMF correlations (b)"

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