Scientia Agricultura Sinica ›› 2022, Vol. 55 ›› Issue (21): 4131-4143.doi: 10.3864/j.issn.0578-1752.2022.21.004

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY·AGRICULTURE INFORMATION TECHNOLOGY • Previous Articles     Next Articles

Coupling Effects of N-fertilizer Postponing Application and Intercropping on Maize Photosynthetic Physiological Characteristics

XU Ke(),FAN ZhiLong,YIN Wen,ZHAO Cai,YU AiZhong,HU FaLong(),CHAI Qiang()   

  1. College of Agronomy, Gansu Agricultural University/State Key Laboratory of Aridland Crop Science, Lanzhou 730070
  • Received:2022-01-04 Accepted:2022-06-02 Online:2022-11-01 Published:2022-11-09
  • Contact: FaLong HU,Qiang CHAI E-mail:xk9417@126.com;hufl@gsau.edu.cn;chaiq@gsau.edu.cn

Abstract:

【Objective】The problem for film-mulched maize in the oasis irrigation region was an advanced nitrogen (N) requirement and led to the insufficient supply of N at the late growth stages. In this study, the effects of N-fertilizer postponing application on intercropped maize photosynthetic physiological characteristics and grain yield were studied, so as to reveal the photosynthetic mechanism of intercropped maize grain yield advantage in the experimental area. 【Method】From 2019 to 2021, the maize was used as experimental materials in Hexi oasis irrigation region. The split-plot experiment design was adopted, among which pea/maize intercropping and maize monoculture were the main factors, and three N fertilizer postponing application (postponing ration 20%, 10%, and traditional practice) were the secondary factors. Then, this field experiment was used to investigate the photosynthetic physiological characteristics and yield performance of maize under N-fertilizer postponing application and intercropping pattern. 【Result】The results demonstrated that the grain yield of intercropped maize under the postponing application of 20% N-fertilizer and 10% was increased by 28.5% and 13.8%, and biomass yield by 23.8% and 12.5%, respectively, compared with traditional N management practices. Similarly, compared with traditional N management practice, the grain yield of sole maize under the postponing application of 20% N-fertilizer and 10% was also increased by 29.7% and 13.3%, and biomass yield by 19.6% and 10.3%, respectively. Compared with the monoculture maize, intercropping could increase the grain yield by 33.2%-35.1% and biomass yield by 26.8%-31.5% under the same area. Furthermore, the postponing application of 20% N-fertilizer and 10% increased the population grain yield of intercropping pattern by 27.2% and 12.9%, respectively, compared with the traditional N management practice. The results showed that intercropping pattern could increase the grain yield of maize compared with the sole pattern, and the N fertilizer postponing application also boosted the improvement of grain yield in the intercropping system compared with the traditional N management practice. During the whole growth periods, the intercropping increased the net photosynthetic rate, stomatal conductance, transpiration rate, and decreased intercellular CO2 concentration. Compared with traditional N management, the net photosynthetic rate under the postponing application of 20% N-fertilizer and 10% was increased by 12.8% and 6.0%, the stomatal conductance by 14.0% and 6.9%, and the transpiration rate by 20.5% and 9.5%, respectively, while the intercellular CO2 concentration was decreased by 29.8% and 13.1%, respectively. The SPAD value under the postponing application of 20% N-fertilizer and 10% was increased by 7.5% and 3.7%, respectively. The principal component analysis results showed that the N-fertilizer postponing application and intercropping pattern could increase the grain yield via boosting the net photosynthetic rate, the stomatal conductance, the transpiration rate, and leaf SPAD value, and decreasing intercellular CO2 concentration. 【Conclusion】N-fertilizer postponing application 20% treatment (36 kg·hm-2 N fertilizer was topdressing at maize jointing stage and 108 kg·hm-2 at 15 d post-silking stage) had the advantage of improving the photosynthetic characteristics of intercropped maize, thereby boosting the grain yield improvement.

Key words: Zea mays, physiological characteristics, N-fertilizer postponing application, intercropping

Fig. 1

The pattern of pea//maize strip intercropping and maize monoculture"

Table 1

Total amount of N fertilizer and allocation amount under each treatment (kg·hm-2)"

种植模式
Cropping system
代码
Code
基肥
Base fertilizer
追肥N fertilizer topdressing (kg·hm-2) 后移比例
Postponed percentage
总施氮量
Total fertilizer
拔节期
Jointing
大喇叭口期Pre-tasseling 吐丝后15 d
15 d after silking
间作
Intercropping
IN1 42 21 83 63 20% 209
IN2 42 42 83 42 10% 209
IN3 42 63 83 21 209
单作
Monoculture
SN1 72 36 144 108 20% 360
SN2 72 72 144 72 10% 360
SN3 72 108 144 36 360

Fig. 2

Temporal dynamics of photosynthetic rate of maize under different N managements The determination dates on maize plants were 3 June, 18 June, 3 July, 18 July, 2 August, and 17 August in 2019, 8 June, 23 June, 8 July, 23 July, 7 August, and 22 August in 2020, and 5 June, 20 June, 5 July, 20 July, 4 August, and 19 August in 2021, respectively. The corresponding growing stages of maize were jointing, pre-tasseling, tasseling, silking, early-filling, and middle-filling stage, respectively. The same as below"

Fig. 3

Temporal dynamics of stomatal conductance of maize under different N mangements"

Fig. 4

Temporal dynamics of cellular CO2 concentration of maize under different N managements"

Fig. 5

Temporal dynamics of transpiration rate of maize under different N managements"

Fig. 6

Temporal dynamics of SPAD of maize under different N managements"

Table 2

Grain yield and harvest index of maize under different cropping patterns and N fertilizer management"

处理
Treatment
籽粒产量Grain yield (kg·hm-2) 生物产量Biomass (kg·hm-2) 收获指数Harvest index
2019 2020 2021 2019 2020 2021 2019 2020 2021
玉米
Maize
总产量
Total
玉米
Maize
总产量
Total
玉米
Maize
总产量
Total
玉米
Maize
总产量
Total
玉米
Maize
总产量
Total
玉米
Maize
总产量
Total
玉米
Maize
IN1 10508b 12917ab 11084c 13626b 10664c 13103ab 22505d 27401b 23416d 28665b 22630d 28031b 0.467a 0.473a 0.471a
IN2 9523b 11785b 9349d 11587cd 9707cd 11817bc 20914e 25560c 20160e 24887d 21186e 25997c 0.455ab 0.464a 0.459a
IN3 8136c 10228c 8309e 10443e 8659d 10483d 17979f 22380d 18265f 22868e 19110f 23435d 0.453ab 0.456a 0.454a
SN1 13575a 13575a 14537a 14537a 13692a 13692a 29340a 29340a 31325a 31325a 29502a 29502a 0.462a 0.464a 0.464a
SN2 12429a 12429ab 11903b 11903c 12192b 12192b 27434b 27434b 26748b 26748c 27596b 27596b 0.453ab 0.445a 0.442a
SN3 10529b 10529c 10924c 10924de 10772c 10772cd 25131c 25131c 25029c 25029d 25221c 25221c 0.419b 0.437a 0.427a
显著性 Significance
种植模式
Cropping
system (C)
** NS ** * ** NS ** ** ** ** ** ** NS NS NS
施氮制度
Nitrogen management (N)
** ** ** ** ** ** ** ** ** ** ** ** NS NS NS
种植模式×施氮制度(C×N) NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS

Fig. 7

Principal component analysis of different indices Pn, Gs, Ci, Tr, SPAD, GY, and BY represent photosynthetic rate, stomatal conductance, intercellular CO2 concentration, transpiration rate, SPAD value, grain yield, and biomass yield, respectively. IM and SM represent intercropped maize and monoculture maize, respectively"

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