Scientia Agricultura Sinica ›› 2019, Vol. 52 ›› Issue (6): 1058-1065.doi: 10.3864/j.issn.0578-1752.2019.06.009

• HORTICULTURE • Previous Articles     Next Articles

Physiological Mechanism of S-adenosylmethionine on Alleviating Chlorothalonil Residue in Tomato

GU ChaoHeng,YAN YanYan,WEI XiYa,SHI QingHua,GONG Biao()   

  1. College of Horticultural Science and Engineering, Shandong Agricultural University/State Key Laboratory of Crop Biology / Ministry of Agriculture Key Laboratory of Horticultural Crop Biology and Germplasm Creation in Huang-Huai Region, Tai’an 271018, Shandong
  • Received:2018-11-18 Accepted:2019-01-14 Online:2019-03-16 Published:2019-03-22
  • Contact: Biao GONG E-mail:gongbiao@sdau.edu.cn

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Abstract:

【Objective】 In order to provide basic mechanism for vegetable safe production, this study aimed at revealing the physiological mechanism of S-adenosylmethionine (SAM)-induced pesticide metabolism. 【Method】 The fresh-eating tomato ‘QianXi’ and broad-spectrum pesticide ‘chlorothalonil, CHT’ were selected as the experimental materials. Then, we studied the effects of exogenous SAM on the metabolism of pesticide, reactive oxygen species and glutathione. 【Result】 The results showed that the CHT residues were significantly reduced by exogenous spraying 0.5-2 μmol·L -1 SAM. However, no significantly changes could be observed when the concentrations of SAM arrived at 0.5 μmol·L -1. Compared with control, the activities of glutathione S-transferase (GST), glutathione reductase (GR) and dehydroascorbate reductase (DHAR) were significantly increased under CHT treatment. And similar results could be obtained with the contents of glutathione (GSH), oxidized glutathione (GSSG) and total glutathione (GSH+GSSG). The GSH/GSSG were induced by CHT firstly, and then decreased. At the same time, superoxide anion ($\mathop{{O}}_{2}^{{\mathop{}_{\ ·}^{-}}}$) and hydrogen peroxide (H2O2) were significantly induced by CHT. However, no significant changes were observed in malondialdehyde (MDA) content. Importantly, exogenous application of 0.5 μmol·L -1 SAM had significant effects on improving the activities of GST, GR and DHAR, as well as contents of GSH and GSH+GSSG. Similarly, no significant changes were observed in MDA contents among different treatments. 【Conclusion】 Taken together, exogenous application of 0.5 μmol·L -1 SAM could active the key enzymes of GR and DHAR to improve the glutathione cycle and GSH regeneration, which induced the CHT metabolism of tomato fruit depending on GST, $\mathop{{O}}_{2}^{{\mathop{}_{\ ·}^{-}}}$, and H2O2 pathway.

Key words: tomato, pesticide metabolism, S-adenosylmethionine, reactive oxygen species, glutathione metabolism

Fig. 1

Effects of different concentrations of SAM on CHT contents of tomato fruits Different letters meant significant difference among treatments at 0.05 level. The same as below"

Fig. 2

Effects of CHT and CHT+SAM on contents of$\mathop{{O}}_{2}^{{\mathop{}_{\ ·}^{-}}}$, H2O2 and MDA of tomato fruits"

Fig. 3

Effects of CHT and CHT+SAM on activities of GR and DHAR of tomato fruits"

Fig. 4

Effects of CHT and CHT+SAM on contents of GSH, GSSG, GSH+GSSG and GSH/GSSG of tomato fruits"

Fig. 5

Effects of CHT and CHT+SAM on GST activity of tomato fruits"

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