陆地棉叶片叶绿素含量与SSR标记的关联分析及优异等位变异的挖掘

doi:10.3864/j.issn.0578-1752.2017.18.001

Abstract

Abstract: 【Objective】The objective of this study is to detect the SSR markers associated with leaf chlorophyll content and explore the alleles and their typical materials in upland cotton. The results will be helpful for molecular marker-assisted breeding.【Method】The natural population including 185 upland cotton accessions were planted at two different places in 3 years and the data of leaf chlorophyll content were recorded at 3 stages (0, 10 and 20 days after topping) every year. In analysis the polymorphism information of population structure, Power Marker 3.25 software was used to estimate the polymorphism information content (PIC). The structure of the natural population was analyzed using STRUCTURE 2.3.4 software and the kinship was estimated using TASSEL 3.0 software. Then the data were associated with 137 SSR markers by GLM (general linear model, Q) and MLM (mixed linear model, Q+K). The superior alleles were exploited and the phenotypic effects of total alleles were found.【Result】 Totally 355 polymorphic alleles were found with 137 SSR markers and 2.6 alleles were revealed with each marker in average ranged from 0.01-0.95. The 85% of total alleles were highly polymorphic primers (PIC＞0.5). HAU2146 (PIC=0.95) and NAU2083 (PIC=0.93) kept the maximum PIC. According to the results of STRUCTURE software, K value was 2 when ΔK was the max so the cultivars were divided into 2 populations. A total of 22 alleles found by GLM method significantly at the level of P＜0.001 which explained 5.28%-10.85% of the phenotypic variance and the mean value was 7.24%. SWU0529a (R² =10.85%) and NAU998c (R²=10.48%) kept the max value. Meanwhile, 17 alleles were found by MLM method significantly at the level of P＜0.01 which explained 3.72%-8.58% of the phenotypic variance and the mean value was 4.72%. SWU0923b (R²=8.06%) and SWU0662d (R²=6.74%) kept the max value. A total of 12 alleles were revealed by GLM method and MLM method in common, and NAU998c was significantly at 3 stages by GLM and MLM methods. Two positive alleles (HAU3318b and SWU0987b) were revealed over the estimated phenotypic effects. The 53 carrier materials of two positive alleles kept higher SPAD value in average than the 46 materials carried none of two positive alleles in 10 and 20 days after topping.【Conclusion】A total of 12 alleles associated significantly with leaf chlorophyll of upland cotton were found, and then two positive superior alleles, 53 carrier materials and one typical materials were revealed.

Key words: upland cotton, chlorophyll, SSR, association analysis, superior allele

LIU QiBao, LI LiBei, ZHANG Chi, SU JunJi, WEI HengLing, WANG HanTao, YU ShuXun. Association Analysis of Leaf Chlorophyll Content with SSR Markers and Exploration of Superior Alleles in Upland Cotton[J].Scientia Agricultura Sinica, 2017, 50(18): 3439-3449.

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