Scientia Agricultura Sinica ›› 2017, Vol. 50 ›› Issue (2): 348-361.doi: 10.3864/j.issn.0578-1752.2017.02.013

• STORAGE·FRESH-KEEPING·PROCESSING • Previous Articles     Next Articles

Volatile Components of Main Indica Rice Cultivars from Hunan Province

SONG Wei, HU WanJun, XU ZongJi, LIU Bing, FAN Yan, NI Yang, YANG HuiPing   

  1. Nanjing University of Finance and Economics /Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210023
  • Received:2016-05-13 Online:2017-01-16 Published:2017-01-16

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Abstract: 【Objective】The volatile components of different indica rice varieties from Hunan province were investigated and compared to explore the possibility that using volatile compounds as indicators for characterization and differentiation of varieties, also for providing a reference for rice storage, processing and quality control.【Method】The head space-solid phase microextraction method was used to extract the volatile components from twelve varieties of indica rice and the extracted volatile substances were analyzed by gas chromatography-mass spectrometry method. The principal component analysis was adopted to analyze their flavors.【Result】A total of 265 volatile components were detected, 125 kinds of substances were screened according to the quality of not less than 80, including 56 kinds of hydrocarbons, 12 kinds of alcohols, 13 kinds of aldehydes, 14 kinds of ketones, 19 kinds of eaters, and 11 kinds of heterocylic compounds. The average percentage contribution of alkanes, olefin, aromatic hydrocarbons, alcohols, aldehydes, ketones, eaters and heterocylic compounds to the total were 26.907%, 8.417%, 7.308%, 5.810%, 9.628%, 18.626%, 13.926% and 6.819%, respectively. The coefficient of variation was 19.98%, 38.22%, 34.67%, 41.12%, 28.57%, 38.81%, 20.13% and 34.33%, respectively. High levels of 6, 10, 14-trimethyl-2-pentadecanone were detected in all species. Some varieties have unique substances, and Kauri-15-ene only existed in Zhuliangyou 819 and Zaoyou, Tezao has a special bisabolene. Isophytol, tetrahydrofurfuryl lavender alcohol and citronellol existed only in Zhuliangyou819, Tezao and Zaoyou respectively. Only Zaoxian87 contained high levels of dihydroxyacetophenone. Benzoicacid ester of silicon occurred only in Zaoyou, chloroformate n-nonyl ester was only detected in Zhongzao 39, and chloroformate n-octyl phthalate was detected only in Zhuliangyou 819 and Zaoxain 51-4. The results of principal component analysis showed that olefin, alkanes, aldehydes and heterocylic substances were the main influencing factors. The contribution rate was 35.878%, 19.267%, 18.727% and 13.596%, respectively, and the cumulative contribution rate was 87.467%. Samples (Xiangai45, Xiangzao32, Zaoxian87, Zaoxian89, Zaoxian51-4, Zhuliangyou 819, Tezao, Zhongzao39, Zaoyou) were distinguished except Zhuliangyou402, Tanliangyou83 and Zaoxian24 according to olefin. Zaoxian 87, Zaoxian89 and Zaoxian51-4 could be identified by alkanes. Zhuliangyou 819 and Zaoixan 51-4 were distinguished through aldehydes. Zaoxian 51-4 and Zaoxian 24 were identified in accordance with heterocyclic substance.【Conclusion】There are significant differences in volatile compositions and concentrations among 12 varieties of indica rice. Principal component analysis can identify specific substances and distinct paddy varieties.

Key words: Hunan province, indica rice, volatile compounds, principal component analysis

[1]    Champagne E T. Rice aroma and flavor: a literature review. Cereal Chemistry, 2008, 85(4): 445-454.
[2]    刘敬科, 郑理, 赵思明, 熊善柏. 蒸煮方法对米饭挥发性成分的影响. 中国粮油学报, 2007, 22(5): 12-15.
Liu J K, Zheng L, Zhao S M, Xiong S B. Effect of cooking method on volatile compounds of cooked rice. Journal of the Chinese Cereals and Oils Association, 2007, 22(5): 12-15. (in Chinese)
[3]    杨慧萍, 乔琳, 李冬珅, 宋伟. 基于GC-MS的粳稻谷糙米大米挥发性成分差异性研究. 食品工业科技, 2016, 37(3): 317-322.
Yang H P, Qiao L, Li D S, Song W. Study on the difference of volatile substances among the japonica brown rice white rice based on GC-MS. Science and Technology of Food industry, 2016, 37(3): 317-322. (in Chinese)
[4]    宋伟, 张明, 张婷筠. 基于GC/MS的储藏粳稻谷挥发物质变化研究. 中国粮油学报, 2013, 28(11): 97-102.
Song W, Zhang M, Zhang T J. Study on the changes of volatile substances in the storage japonica based on GC-MS. Journal of the Chinese Cereals and Oils Association, 2013, 28(11): 97-102. (in Chinese)
[5]    宋伟, 张明, 甘小玉, 陆蕊. 储藏粳稻挥发物质与质量指标关系的回归分析. 中国粮油学报, 2014, 29(11): 97-103.
Song W, Zhang M, Gan X Y, Lu R. Regression analysis of relationship of quality indexes and volatile compounds in japonica rice during storage. Journal of the Chinese Cereals and Oils Association, 2014, 29(11): 97-103. (in Chinese)
[6]    Maga J A. Cereal volatiles: A review. Journal of Agricultural and Food Chemistry, 1978, 26(1): 175-178.
[7]    Laohakunjit N, Kerdchoechuen O. Aroma enrichment and the change during storage of non-aromatic milled rice coated with extracted natural flavor. Food Chemistry, 2007, 101(1): 339-344.
[8]    Bryant R J, McClung A M. Volatile profiles of aromatic and non-aromatic rice cultivars using SPME/GC–MS. Food Chemistry, 2011, 124(2): 501-513.
[9]    郭亚娟, 邓媛元, 张瑞芬, 张名位, 魏振承, 唐小俊, 张雁. 不同荔枝品种果干挥发性物质种类及其含量比较. 中国农业科学, 2013, 46(13): 2751-2768.
Guo Y J, Deng Y Y, Zhang R F, Zhang M W, Wei Z C, Tang X J, Zhang Y. Comparison of volatile components from different varieties of dried Litchi. Science Agriculyura Sinica, 2013, 46(13): 2751-2768. (in Chinese)
[10]   梁静, 杨冬梅, 田向荣, 谢文华, 李俊年. 永顺颗砂贡米香气成分的气相色谱-质谱分析. 食品科学, 2014, 35(8): 236-239.
Liang J, Yang D M, Tian X R, Xie W H, Li J N. GC-MS analysis of aroma compounds of Kesha Tribute Rice from Yongshun. Food Science, 2014, 35(8): 236-239. (in Chinese)
[11]   Grimm C C, Bergman C J, Delgado J T. Screening for 2-acetyl-1-pyrroline in the headspace of rice using SPME/GC-MS. Journal of Agriculture and Food Chemistry, 2001, 49(1): 245-249.
[12]   宋伟, 谢同平, 张美玲, 马宁. 应用电子鼻判别不同储藏条件下粳稻谷品质的研究. 中国粮油学报, 2012, 27(5): 92-96.
Song W, Xie T P, Zhang M L, Ma N. Distinguishing the quality of japonica rice stored at different storage conditions by using electronic nose. Journal of the Chinese Cereals and Oils Association, 2012, 27(5): 92-96. (in Chinese)
[13]   邹小波, 赵杰文. 电子鼻快速检测谷物霉变的研究. 农业工程学报, 2004, 20(4): 121-124.
Zou X B, Zhao J W. Rapid identification of moldy corn by electronic nose. Transactions of the Chinese Society of Agricultural Engineering, 2004, 20(4): 121-124. (in Chinese)
[14]   Bryant R J, McClung A M. Volatile profiles of aromatic and non-aromatic rice cultivars using SPME/GC-MS. Food Chemistry, 2011, 124(2): 501-513.
[15]   苗菁, 苏慧敏, 张敏. 米饭中关键风味化合物的分析. 食品科学, 2016, 37(2): 82-86.
Miao J, Su H M, Zhang M. Analysis of the key flavor compounds in cooked rice. Food science, 2016, 37(2): 82-86. (in Chinese)
[16]   林家永, 高艳娜, 吴胜芳, 王松雪. 顶空固相微萃取-气质联用法分析稻谷挥发性成分. 食品科学, 2009(20): 277-282.
Lin J Y, Gao Y N, Wu S F, Wang S X. Headspace solid phase microextraction coupled to GC-MS for analyzing volatile components in paddy. Food Science, 2009(20): 277-282. (in Chinese)
[17]   宋江峰, 李大婧, 刘春泉, 刘玉花. 甜糯玉米软罐头主要挥发性物质主成分分析和聚类分析. 中国农业科学, 2010, 43(10): 2122-2131.
Song J F, Li D J, Liu C Q, Liu Y H. Principal components  analysis and cluster analysis of flavor compositions in waxy corn  soft can. Scientla Agricultura Sinica, 2010, 43(10): 2122-2131. (in Chinese)
[18] Mahattanatawee K, Rouseff R L. Comparison of aroma active and sulfur volatiles in three fragrant rice cultivars using GC-Olfactometry and GC-PFPD. Food chemistry, 2014, 154: 1-6.
[19]   王彦蓉, 崔春, 赵谋明. 固相微萃取与气质联用法分析沙琪玛中的风味成分. 现代食品科技, 2011, 27(11): 1406-1409.
Wang Y R, Cui C, Zhao M M. Seperation and identification of volatile flaovrs sachima uisng Solid-Phase Microextraction Gas Chromatrograph-mass Spectrometry (SPME-GC-MS). Modern Food Science & Technology, 2011, 27(11): 1406-1409. (in Chinese)
[20]   Zeng Z, Zhang H, Zhang T, TAMOGAMI A, CHEN J Y. Analysis of flavor volatiles of glutinous rice during cooking by combined gas chromatography–mass spectrometry with modified headspace solid-phase microextraction method. Journal of Food Composition and Analysis, 2009, 22(4): 347-353.
[21]   Kong Z, Zhao D. The inhibiting effect of abscisic acid on fragrance of Kam Sweet rice. Journal of Food and Nutrition Research, 2014, 2(4): 148-154.
[22]   马良, 张乃建, 王若兰. 玉米窝头挥发性成分分析. 粮食与油脂, 2015, 28(8): 42-44.
Ma L, Zhang N J, Wang R L. Analysis of volatile compounds steamed corn bum. Cereals & Oils, 2015, 28(8): 42-44. (in Chinese)
[23]   Tananuwong K, Lertsiri S. Changes in volatile aroma compounds of organic fragrant rice during storage under different conditions. Journal of the Science of Food and Agriculture, 2010, 90(10): 1590-1596.
[24]   Guo Q, Wu W, Massart D L, Boucon C, Jong S D. Feature selection in principal component analysis of analytical data. Chemometrics and Intelligent Laboratory Systems, 2002, 61(1): 123-132.
[25]   Tian X Y, Cai Q, Zhang Y M. Rapid classification of hairtail fish and pork freshness using an electronic nose based on the PCA method. Sensors, 2011, 12(1): 260-277.
[26]   Sebzalli Y M, Wang X Z. Knowledge discovery from process operational data using PCA and fuzzy clustering. Engineering Applications of Artificial Intelligence, 2001, 14(5): 607-616.
[27]   余莉, 张时龙, 李清超, 杨珊, 王昭礼, 吴宪志, 卢运, 赵龙. 主成分分析在芸豆品种筛选中的应用. 东北农业科学, 2016, 41(1): 91-96.
Yu L, Zhang S L, li Q C, Yang S, Wang Z L, Wu X Z, Lu Y, Zhao L. Application of principal component analysis in the screening of Kidney Bean varities. Journal of Dongbei Agricultural Sciences, 2016, 41(1): 91-96. (in Chinese)
[28]   王培璇, 毛健, 李晓钟, 刘芸雅, 孟祥勇. 不同地区黄酒挥发性物质差异性分析. 食品科学, 2014, 35(6): 83-89.
Wang P X, Mao J, Li X Z, Liu Y Y, Meng X Y. Comparative analysis of volatile components of Chineses rice wines from different areas. Food Science, 2014, 35(6): 83-89. (in Chinese)
[29]   Griglione A, Liberto E, Cordero C, Bressanello D, Cagliero C, Rubiolo P, Bicchi C, Sgorbini B. High-quality Italian rice cultivars: Chemical indices of ageing and aroma quality. Food chemistry, 2015, 172: 305-313.
[30]   Frank T, Reichardt B, Shu Q, ENGEL K H. Metabolite profiling of colored rice (Oryza sativa L.) grains. Journal of Cereal Science, 2012, 55(2): 112-119.
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