施硫对不结球白菜硝酸盐累积及氮硫同化关键基因表达的影响

doi:10.3864/j.issn.0578-1752.2016.11.018

Abstract

Abstract: 【Objective】The objective of this paper is to identify the best sulfate to reduce nitrate accumulation in non-heading Chinese cabbage, and analyze the key genes involved in nitrogen and sulphur assimilation. The results of this study will provide new insights into sulphur fertilization and investigation of the molecular mechanisms of nitrate accumulation with the goal of molecular breeding.【Method】Nitrate content of non-heading Chinese cabbage leaves and petioles were measured under treatments of 4 forms of sulphur with 3 concentrations. The expression of 30 nitrogen and sulphur assimilation related genes were analyzed by semi-quantitative RT-PCR. 【Result】Sulphur treatments were significantly increased the aboveground biomass of non-heading Chinese cabbage, and the treatments of Na₂SO₄ had the best effect, and 30 mg·kg^-1Na₂SO₄ was the most effective treatment as the aboveground biomass increased by 49.76% compared with the control. Na₂SO₄and Na₂S₂O₃ reduced nitrate content of non-heading Chinese cabbage more significantly than others. Na₂SO₄decreased nitrate content by 12.23%-23.55% in leaves and by 33.08%-41.98% in petioles compared with the control, and it was also found a positive correlation between the reduction and concentration of Na₂SO₄, and 30 mg·kg^-1Na₂SO₄ also had the best effect. Na₂S₂O₃ decreased nitrate content by 15.34%-33.08% compared with the control in leaves and by 11.95%-19.68% in petioles. Sulphur promoted nitrogen assimilation, and the expression of NR-1, NADH-GOGAT-1, NADH-GOGAT-2, Cytoplasm-GS-4, Cytoplasm-GS-5, and GDH-3 were higher than the control in leaves, and the expression of NR-1, NADH-GOGAT-2, Cytoplasm-GS-1, and GDH-2 were higher than the control in petioles. The expression of NADH-GOGAT-2 was correlated with nitrate levels. Sulphur treatments also had an effect on sulphur assimilation genes, and the expressions of ATPS-2, ATPS-3, ATPS-4, APSR-3, SIR, SAT1.1, and SAT2.1 were higher than the control in leaves, and only the expressions of SIR and OASTL-A were higher than the control in petioles. 【Conclusion】 Na₂SO₄ had the best effect in reducing nitrate content and increasing yield of non-heading Chinese cabbage, and 30 mg·kg^-1 Na₂SO₄was the most effective treatment. The expression of NADH-GOGAT-2 was correlated with nitrate levels. The data suggested that NADH-GOGAT-2 may be the key gene in nitrogen assimilation.

Key words: non-heading Chinese cabbage, sulphur, nitrate, nitrogen and sulphur assimilation, gene expression

XU Yao, MU Jian-mei, ZHANG Guo-qin, MA Jia-jia, XU Jun, LI Jun, LIU Feng-jun, SHE Xu-dong. Effect of Sulphur Availability on Nitrate Accumulation and Expression of Nitrogen and Sulphur Assimilation Related Genes in Non-Heading Chinese Cabbage[J].Scientia Agricultura Sinica, 2016, 49(11): 2222-2233.

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Effect of Sulphur Availability on Nitrate Accumulation and Expression of Nitrogen and Sulphur Assimilation Related Genes in Non-Heading Chinese Cabbage

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