Scientia Agricultura Sinica ›› 2016, Vol. 49 ›› Issue (11): 2222-2233.doi: 10.3864/j.issn.0578-1752.2016.11.018

• RESEARCH NOTES • Previous Articles     Next Articles

Effect of Sulphur Availability on Nitrate Accumulation and Expression of Nitrogen and Sulphur Assimilation Related Genes in Non-Heading Chinese Cabbage

XU Yao, MU Jian-mei, ZHANG Guo-qin, MA Jia-jia, XU Jun, LI Jun, LIU Feng-jun, SHE Xu-dong   

  1. Institute of Agricultural Sciences in Taihu Lake District, Suzhou 215155, Jiangsu
  • Received:2015-11-16 Online:2016-06-01 Published:2016-06-01

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Abstract: 【Objective】The objective of this paper is to identify the best sulfate to reduce nitrate accumulation in non-heading Chinese cabbage, and analyze the key genes involved in nitrogen and sulphur assimilation. The results of this study will provide new insights into sulphur fertilization and investigation of the molecular mechanisms of nitrate accumulation with the goal of molecular breeding.【Method】Nitrate content of non-heading Chinese cabbage leaves and petioles were measured under treatments of 4 forms of sulphur with 3 concentrations. The expression of 30 nitrogen and sulphur assimilation related genes were analyzed by semi-quantitative RT-PCR. 【Result】Sulphur treatments were significantly increased the aboveground biomass of non-heading Chinese cabbage, and the treatments of Na2SO4 had the best effect, and 30 mg·kg-1 Na2SO4 was the most effective treatment as the aboveground biomass increased by 49.76% compared with the control. Na2SO4 and Na2S2O3 reduced nitrate content of non-heading Chinese cabbage more significantly than others. Na2SO4 decreased nitrate content by 12.23%-23.55% in leaves and by 33.08%-41.98% in petioles compared with the control, and it was also found a positive correlation between the reduction and concentration of Na2SO4, and 30 mg·kg-1 Na2SO4 also had the best effect. Na2S2O3 decreased nitrate content by 15.34%-33.08% compared with the control in leaves and by 11.95%-19.68% in petioles. Sulphur promoted nitrogen assimilation, and the expression of NR-1, NADH-GOGAT-1, NADH-GOGAT-2, Cytoplasm-GS-4, Cytoplasm-GS-5, and GDH-3 were higher than the control in leaves, and the expression of NR-1, NADH-GOGAT-2, Cytoplasm-GS-1, and GDH-2 were higher than the control in petioles. The expression of NADH-GOGAT-2 was correlated with nitrate levels. Sulphur treatments also had an effect on sulphur assimilation genes, and the expressions of ATPS-2, ATPS-3, ATPS-4, APSR-3, SIR, SAT1.1, and SAT2.1 were higher than the control in leaves, and only the expressions of SIR and OASTL-A were higher than the control in petioles. 【Conclusion】 Na2SO4 had the best effect in reducing nitrate content and increasing yield of non-heading Chinese cabbage, and 30 mg·kg-1 Na2SO4 was the most effective treatment. The expression of NADH-GOGAT-2 was correlated with nitrate levels. The data suggested that NADH-GOGAT-2 may be the key gene in nitrogen assimilation.

Key words: non-heading Chinese cabbage, sulphur, nitrate, nitrogen and sulphur assimilation, gene expression

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