淮稻5号的真菌多样性及其储藏过程中可培养的优势真菌

doi:10.3864/j.issn.0578-1752.2016.07.013

Abstract

Abstract: 【Objective】The objective of this study is to determine the fungal genus in 2013 Huaidao No. 5 rice by traditional morphological observation and molecular biology method, as well as to investigate the dominant fungal strains on Huaidao No.5 rice under different storage conditions. Results of this study could provide a scientific basis for the fungal genus identification and control of fungi growth in Huaidao No. 5 rice. 【Method】The culturable fungal strains were obtained by using both Rose Bengal and potato dextrose agar (PDA) media, following the instruction of GB 4789.15-2010. The Huaidao No. 5 rice collected from 4 different regions were mixed and then the moisture content were adjusted to 14.5% and 18.5% respectively. The samples were stored in the artificial climate equipment under 25℃. The culturable dominant fungi were determined by traditional culturing of the rice samples monthly removed from the simulated storage conditions. The fungal genus were identified by morphological observation of colonies, hyphae and spores by referencing to the . At the same time, the internal transcribed spacer (ITS) and 28S rDNA were amplified by PCR, and then ligated into T vector and sequenced. The fungal species were identified by constructing phylogenetic trees.【Result】In terms of fungal diversity in Huaidao No. 5 rice: 33 strains of fungi were isolated from four regions of the 2013 Huaidao No. 5 rice, mainly for Penicillium, Aspergillus and Fusarium, including 16 strains of Penicillium and 15 strains of Aspergillus, accounting for the separated strains of 48.5% and 45.5%, respectively, indicating that Penicillium and Aspergillus are the dominant fungi genus in 2013 Huaidao No. 5 rice. There are only 2 strains of Fusarium, which accounted for only 6% of the isolates. Simulation storage experiment results showed that Penicillium aurantiogriseum appeared to be the dominant strain from 6 month in moisture content of 14.5% for Huaidao No. 5 rice under 25℃ storage, and this strain persisted to be dominant in 7 and 8 months storage. While for the Huaidao No. 5 rice with a moisture content of 18.5%, a yeast strain appeared to be the dominant from 7 month and this strain persisted to be dominant at 8 month under 25℃ storage.【Conclusion】 33 strains of fungi were separated and identified from 2013 Huaidao No. 5 rice, including 16 strains of Penicillium and 15 strains of Aspergillus, respectively, indicating that Penicillium and Aspergillus are the dominant fungi genus in 2013 Huaidao No. 5 rice. And under different simulated storage conditions, different cultural fungal strains dominated the Huaidao No. 5 rice with different moisture content.

Key words: rice, storage, fungi, ITS, 28S rDNA, flora

DU Li-hui, HE Xiao-ying, LIU Ling-ping, YUAN Jian, JU Xing-rong. Fungal Diversity of Huaidao No. 5 Rice and the Dominant Culturable Fungal Strains During Storage[J].Scientia Agricultura Sinica, 2016, 49(7): 1371-1381.

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Fungal Diversity of Huaidao No. 5 Rice and the Dominant Culturable Fungal Strains During Storage

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