橘小实蝇幼虫解毒酶系基因应对高效氯氰菊酯胁迫的组织特异性表达

doi:10.3864/j.issn.0578-1752.2015.19.008

Abstract

Abstract: 【Objective】Based on the transcriptome data of Bactrocera dorsalis, the aim of this study is to systematically analyze the expression of genes in larvae encoding detoxification enzymes under β-cypermethrin exposure, identify important detoxification genes, and focus on the discussion of biological significance of tissue specific expression of these genes. 【Method】 The expressions of 30 detoxification genes in the whole body of larvae, midgut, and fat body were detected under β-cypermethrin exposure by using qPCR.【Result】Under the stress of β-cypermethrin, the qPCR results of 8 GSTs genes showed that the mRNA levels of 4 Delta class genes significantly increased. The expressions of genes from Delta class were up-regulated to 5.6-32.5 folds in the midgut and fat body, but the expressions of other GST genes did not show very active reaction when exposed to β-cypermethrin. Meanwhile, although the expressions of Delta class GSTs genes significantly increased in midgut and fat body, the expression changes of these genes were not so apparent in the whole body. As for the 6 CCEs genes, their expressions were up-regulated in some degree in the whole body, and α-E3 was up-regulated to 4.1 folds. However, in the midgut and fat body, the expressions of these genes did not increase significantly. The qPCR results of 16 P450s genes showed that the expressions of most P450s genes were up-regulated, especially the genes from CYP4 and CYP6 families. In CYP4 family, the expressions of CYP4D47 (midgut), CYP4E9 (whole body and fat body), CYP4P5 (whole body and fat body) were highly up-regulated. The expression of CYP4AD1 increased in some degree in the midgut and fat body, but no significant changes were found in the whole body. CYP4S18 and CYP4AC4 did not show any positive reaction or their expression changes were not apparent. In CYP6 family, the expressions of CYP6A48 and CYP6A41 had a significant increase in the fat body, 18.0 and 9.6 folds up-regulated, respectively. The expression of others like CYP6G6 and CYP6A50 also increased. The expression of CYP6EK1 was up-regulated by 4.8 folds in the whole body, but did not change in midgut and fat body. The expressions of the other genes, such as CYP12C2, CYP12N1, CYP9F6, and CYP309B1 also significantly increased in the fat body when exposed to β-cypermethrin. But the expression of CYP317B1 did not change under the stress.【Conclusion】When under the stress of β-cypermethrin, the detoxification genes in B. dorsalis larvae were highly enriched in midgut and fat body, but showed a balance in the whole body. It suggests that when under the toxic stress, the pests may have special strategy for efficient use and proper distribution of energy, which increase the energy efficiency to decrease the extra energy cost to resist the damage of exogenous toxin. This kind of strategy might be an important acclimation of insects to avoid fitness cost when under the environmental pressures.

Key words: Bactrocera dorsalis, GSTs, P450s, CCEs, toxic stress, tissue specificity

SHEN Guang-mao, WANG Xiao-na, HUANG Yong, DOU Wei, WANG Jin-jun. Tissue Specific Expression of Genes Encoding Detoxification Enzymes in the Larvae of Bactrocera dorsalis Under β-Cypermethrin Stress[J].Scientia Agricultura Sinica, 2015, 48(19): 3857-3865.

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Tissue Specific Expression of Genes Encoding Detoxification Enzymes in the Larvae of Bactrocera dorsalis Under β-Cypermethrin Stress

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