Scientia Agricultura Sinica ›› 2010, Vol. 43 ›› Issue (4): 873-880 .doi: 10.3864/j.issn.0578-1752.2010.04.028

• RESEARCH NOTES • Previous Articles    

Identification and Eukaryotic Expression of GSTe-3 Gene of Silkworm (Bombyx mori)

PAN Min-hui, XU Yuan, YU Quan-you, LIU Jia, LIU Di, ZHAO Dan-hong, LU Cheng
   

  1. （西南大学生物技术学院/农业部蚕桑学重点开放实验室）
  • Received:2009-05-25 Revised:2009-06-19 Online:2010-02-20 Published:2010-02-20
  • Contact: LU Cheng

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Abstract:

【Objective】 Glutathione S-transferases (GSTs) are a superfamily of multifunctional enzymes found in almost all living organisms. They play important roles in detoxification of endogenous and exogenous toxic substance. It will lay a foundation for the detoxification mechanism of silkworm to study on GSTs of Bombyx mori. 【Method】 BmGSTe3 gene was cloned and analyzed by bioinformation and molecular biology method. The expression of BmGSTe3 was analyzed in silkworm by RT-PCR. BmGSTe3 gene was eukaryotic expressed in sf9 cells by Bac-to-Bac Baculovirus Expression System. 【Result】 The BmGSTe3 is composed of 5 exons and 4 introns and its CDS length is 512 bp. BmGSTe3 contains 15 possible transcription regulatory elements in the upstream of 2 500 bp and two domains, which N-terminal domain is composed of 7 motifs, namely b-a-b-a-b-b-a, while C-terminal domain is composed of 5 ? helixs. BmGSTe3 has a conservative Ser catalyse site, belongs to Epsilon family members and express only in hemolymph and brain. BmGSTe3 protein has good GSTs enzyme activity. 【Conclusion】 BmGSTe3 belongs to Epsilon family members and its protein has good GSTs enzyme activity

Key words: Bombyx mori, BmGSTe3, eukaryotic express, GSTs enzyme activity

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