Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (17): 3704-3711.doi: 10.3864/j.issn.0578-1752.2013.17.020

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Polymorphisms of Chicken ApoA5 Gene and Their Associations with Cholesterol Content and Reproductive Traits

 DU  Xiao-Hui, XIONG  Ting, LIU  Jun-Ying, ZHANG  Huai-Yong, WAN  Huo-Fu, ZHU  Qing   

  1. College of Animal Science and Technology, Sichuan Agricultural University, Ya’an 625014, Sichuan
  • Received:2013-05-29 Online:2013-09-01 Published:2013-07-27

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Abstract: 【Objective】To balance the relationship between cholesterol concentration and reproductive traits, the associations among the traits of cholesterol and reproduction and polymorphisms of chicken ApoA5 gene were studied. 【Method】 The reproduction traits were recorded and the cholesterol concentrations of serum (SCC) and egg yolk (YCC) were measured in 434 SD hens at 300th day. Through PCR-SSCP and PCR-RFLP analyses, 6 new SNPs were detected in ApoA5 gene and their associations with the traits of cholesterol and reproduction were analyzed. 【Result】The effects of the single SNP mainly focused on the age of first egg (AFE) and on the SCC. The results of association analysis of the haplotype, constructed with SNP2, SNP4 and SNP5 because of their closer location and consistent effects, were roughly accordant with the single SNP, but the trait differences between haplotype combinations were enlarged. The haplotype combinations (H5H8, H4H8, H5H6, H6H6) with higher SCC and YCC had better reproductive performance, and H1H5, H1H6 and H3H8 with lower SCC and YCC were on the contrary.【Conclusion】Haplotype SNP2-4-5 of ApoA5 gene revealed the differences of cholesterol and reproductive traits among various combinations, and could be used as a molecular markers to improve reproductive traits.

Key words: chicken , ApoA5 gene , polymorphism , cholesterol , reproductive trait , association analysis

[1]Hargis P S. Modifying egg yolk cholesterol in the domestic fowl? a review. World’s Poultry Science Journal, 1988, 44: 17-29.

[2]Griffin H. Manipulation of egg yolk cholesterol: a physiologist's view. ifying egg yolk cholesterol in the domestic fowl? a review. World’s Poultry Science Journal, 1992, 48: 101-112.

[3]Marçais C, Verges B, Charrière S, Pruneta V, Merlin M, Billon S, Perrot L, Drai J, Sassolas A, Pennacchio L A, Fruchart-Najib J, Fruchart J C, Durlach V, Moulin P. ApoA5 Q139X truncation predisposes to late-onset hyperchylomicronemia due to lipoprotein lipase impairment. The Journal of Clinical Investigation, 2005, 115(10): 2862-2869.

[4]Bauer R. Identification of a new binding partner of chicken apolipoprotein A-V[D]. Wien: Universität Wien, 2009.

[5]Dichlberger A, Cogburn L, Nimpf J, Schneider W J. Avian apolipoprotein AV binds to LDL receptor gene family members. Journal of Lipid Research, 2007, 48(7): 1451-1456.

[6]Hiesberger T, Hermann M, Jacobsen L, Novak S, Hodits R A, Bujo H, Meilinger M, Httinger M, Schneider W J, Nimpf J. The chicken oocyte receptor for yolk precursors as a model for studying the action of receptor-associated protein and lactoferrin. The Journal of Biological Chemistry, 1995, 270(31): 18219-18226.

[7]Schneider W J. Vitellogenin receptors: oocyte-specific members of the low-density lipoprotein receptor supergene family. International Review of Cytology, 1996, 166: 103-137.

[8]George R, Barber D, Schneider W. Characterization of the chicken oocyte receptor for low and very low density lipoproteins. The Journal of Biological Chemistry, 1987, 262(35): 16838-16847.

[9]Elkin R, Yan Z H, Zhong Y, Donkin S S, Buhman K K, Story J      A, Turek J J, Porter R E, Anderson M, Homan R, Newton R S.  Select 3-hydroxy-3-methylglutaryl-coenzyme a reductase inhibitors vary in their ability to reduce egg yolk cholesterol levels in     laying hens through alteration of hepatic cholesterol biosynthesis and plasma VLDL composition. The Journal of Nutration, 1999, 129: 1010-1019.

[10]丁雪梅, 张克英. 产蛋鸡胆固醇代谢及其影响因素. 中国饲料, 2007, 12: 17-20.

Ding X M, Zhang K Y. The cholesterol metabolism and its influencing factors in laying hens. China Feed, 2007, 12: 17-20. (in Chinese)

[11]Elkin R G. Reducing shell egg cholesterol content. II. Review of approaches utilizing non-nutritive dietary factors or pharmacological agents and an examination of emerging strategies. World’s Poultry Science Journal, 2007, 63(1): 5-32.

[12]Pennacchio L A, Olivier M, Hubacek J A, Cohen J C, Cox,D R, Fruchart J C, Krauss R M, Rubin E M. An apolipoprotein influencing triglycerides in humans and mice revealed by comparative sequencing. Science, 2001, 294(5540): 169-173.

[13]Baum L, Tomlinson B, Thomas G. ApoA5‐1131T> C polymorphism is associated with triglyceride levels in Chinese men. Clinical Genetics, 2003, 63: 377-379.

[14]Pennacchio L A, Olivier M, Hubacek J A, Krauss R M, Rubin E M，Cohen J C. Two independent apolipoprotein A5 haplotypes influence human plasma triglyceride levels. Human Molecular Genetics, 2002, 11(24): 3031-3038.

[15]Klos K L E, Hamon S, Clark A G, Boerwinkle E, Liu K, Sing C F. ApoA5 polymorphisms influence plasma triglycerides in young, healthy African Americans and whites of the CARDIA study. Journal of Lipid Research, 2005, 46(3): 564-571. 

[16]Endo K, Yanagi H, Araki J, Herano C,Tomura Y S. Association found between the promoter region polymorphism in the apolipoprotein AV gene and the serum triglyceride level in Japanese schoolchildren. Human Genetics, 2002, 111: 570-572.

[17]Ribalta J, Figuera L, Fernandez-Ballart J, Vilella E, Cabezas M C, Masana L, Joven J. Newly identified apolipoprotein AV gene predisposes to high plasma triglycerides in familial combined hyperlipidemia. Clinical Chemistry, 2002, 48(9): 1597-1600.

[18]van der Vleuten G M, Isaacs A, Zeng W-W, ter Avesta E, Talmudd P J, Dallinga-Thiee G M, van Duijnb C M, Stalenhoefa A F H, de Graaf J. Haplotype analyses of the ApoA5 gene in patients with familial combined hyperlipidemia. Biochimica et Biophysica Acta-Molecular Basis of Disease, 2007, 1772(1): 81-88.

[19]Aouizerat B E, Kulkarni M, Heilbron D, Drown D, Raskin S, Pullinger C R, Malloy M J, Kane J P. Genetic analysis of a polymorphism in the human ApoA-V gene effect on plasma lipids. Journal of Lipid Research, 2003, 44(6): 1167-1173.

[20]李步高, 郭晓红, 曹果清, 高鹏飞, 王效京, 刘宏, 周忠孝. 猪ApoA5基因cDNA的克隆、序列分析及组织表达研究. 畜牧兽医学报, 2011, 42(7): 913-920.

Li B G, Guo X H, Cao G Q, Gao P F, Wang X J, Liu H, Zhou Z X. Molecular cloning, sequence analysis and tissue expression of pig ApoA5 gene. Chinese Journal of Animal and Veterinary Sciences, 2011, 42(7): 913-920. (in Chinese)

[21]Carre W, Wang X, Porter T E, Nys Y, Tang J S, Bernberg E, Morgan R, Burnside J, Aggrey S E, Simon J, Cogburn L A. Chicken genomics resource: sequencing and annotation of 35,407 ESTs from single and multiple tissue cDNA libraries and CAP3 assembly of a chicken gene index. Physiol Genomics, 2006, 25(3): 514-524.

[22]姚俊峰, 张莹, 吴桂琴, 郑江霞, 邓学梅, 杨宁. 鸡ApoA5基因单核苷酸多态性及其与屠体性状的关联研究. 遗传, 2008, 30(5): 607-612.

Yao J F, Zhang Y, Wu G Q, Zheng J X, Deng X M,Yang N. Polymorphisms of chicken ApoA5 gene and associationwith carcass traits of chickens. Hereditas, 2008, 30(5): 607-612. (in Chinese)

[23]刘文芳. 鸡血清胆固醇含量的快速测定. 黑龙江畜牧兽医, 1988, 1: 23.

Liu W F. The rapid determination of the chicken serum cholesterol content. Heilongjiang Animal Science and Veterinary Medicine, 1988, 1: 23. (in Chinese)

[24]王惠芸, 高应. 鸡蛋中胆固醇快速测定方法的研究. 食品科学, 1995, 16: 58-59.

Wang H Y, Gao Y. The study the method of rapid determination of cholesterol in eggs. Food Science, 1995, 16: 58-59. (in Chinese)

[25]Stephens M, Donnelly P. A comparison of bayesian methods for haplotype reconstruction from population genotype data. American Journal of Human Genetics, 2003, 73(5): 1162-1169.

[26]Judson R, Stephens J C. Notes from the SNP vs. haplotype front. Pharmacogenomics, 2001, 2: 7-10.

[27]Weiss K M, Terwilliger J D. How many diseases does it take to map a gene with SNPs? Nature Genetics, 2000, 26(2): 151-158.

[28]杜晓惠, 熊婷, 刘俊莹, 张怀勇, 万火福, 朱庆. 母鸡300日龄血清及蛋黄胆固醇浓度与蛋用性状的关系研究. 中国家禽, 2013, 35(11): 11-15.

Du X H, Xiong T, Liu J Y, Zhang H Y, Wan H F, Zhu Q. Relationship between serum/yolk cholesterol content and egg traits in hens of 300 days age. China Poultry, 2013, 35(11): 11-15. (in Chinese)
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