Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (17): 3704-3711.doi: 10.3864/j.issn.0578-1752.2013.17.020

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Polymorphisms of Chicken ApoA5 Gene and Their Associations with Cholesterol Content and Reproductive Traits

 DU  Xiao-Hui, XIONG  Ting, LIU  Jun-Ying, ZHANG  Huai-Yong, WAN  Huo-Fu, ZHU  Qing   

  1. College of Animal Science and Technology, Sichuan Agricultural University, Ya’an 625014, Sichuan
  • Received:2013-05-29 Online:2013-09-01 Published:2013-07-27

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Abstract: 【Objective】To balance the relationship between cholesterol concentration and reproductive traits, the associations among the traits of cholesterol and reproduction and polymorphisms of chicken ApoA5 gene were studied. 【Method】 The reproduction traits were recorded and the cholesterol concentrations of serum (SCC) and egg yolk (YCC) were measured in 434 SD hens at 300th day. Through PCR-SSCP and PCR-RFLP analyses, 6 new SNPs were detected in ApoA5 gene and their associations with the traits of cholesterol and reproduction were analyzed. 【Result】The effects of the single SNP mainly focused on the age of first egg (AFE) and on the SCC. The results of association analysis of the haplotype, constructed with SNP2, SNP4 and SNP5 because of their closer location and consistent effects, were roughly accordant with the single SNP, but the trait differences between haplotype combinations were enlarged. The haplotype combinations (H5H8, H4H8, H5H6, H6H6) with higher SCC and YCC had better reproductive performance, and H1H5, H1H6 and H3H8 with lower SCC and YCC were on the contrary.【Conclusion】Haplotype SNP2-4-5 of ApoA5 gene revealed the differences of cholesterol and reproductive traits among various combinations, and could be used as a molecular markers to improve reproductive traits.

Key words: chicken , ApoA5 gene , polymorphism , cholesterol , reproductive trait , association analysis

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