利用CDDP标记的菏泽牡丹品种资源的遗传多样性

doi:10.3864/j.issn.0578-1752.2013.13.012

Abstract

Abstract: 【Objective】The objective of this study was to evaluate the genetic diversity and differentiation of Heze tree peony resources at cultivar group level and flower-color population level, respectively.【Method】DNA samples of 299 tree peony cultivars composed of 10 different flower-color populations were amplified by CDDP primers. The 10 populations respectively consisted of white, pink, black, red, yellow, blue, green, fuchsia, purple and complex color cultivars.【Result】With 18 informative and reliable primers, 368 polymorphic loci were detected out of 385 bands in total. At cultivar group level, the Nei’s gene diversity (H), Shannon’s information index (I) and effective number of alleles were 0.1648, 1.2569 and 0.2695, respectively. At population level, the above index was 0.1451, 1.2313 and 0.2306 in turn. Comprehensive analysis indicated that the red population or purple population had higher genetic diversity, while the genetic diversity of the complex-color population or green population was lower. The genetic distance among 10 populations was closer, with an average of 0.0271. The genetic identity was higher, with an average of 0.9735. The coefficient of gene differentiation (Gst) among them was 0.1252, showing that 12.52% genetic variation existed among populations. Gene flow from Gst was 3.4939. Results obtained from genetic diversity analysis and UPGMA dendrogram based Nei’s genetic identity proved the evolutionary trend of tree peony’s flower color at molecular level: pink and red systems might be at the center of Heze tree peony resources, then gradually evolved into fuchsia, purple, blue, white, yellow or black cultivars, while green or complex color cultivars belonged to the degraded color system.【Conclusion】CDDP marker technique can effectively reveal the genetic diversity of tree peony germplasm resources. At cultivar group level, the diversity of Heze tree peony resources was higher than that at flower-color population level. There existed a certain degree of genetic differentiation among the 10 populations. And the clustering results revealed the evolutionary trend of tree peony color.

Key words: tree peony , flower color , CDDP , genetic diversity , genetic differentiation

LI Ying-Ying, ZHENG Cheng-Shu. Genetic Diversity of Tree Peony Cultivar Resources in Heze Revealed by CDDP Marker[J].Scientia Agricultura Sinica, 2013, 46(13): 2739-2750.

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