Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (11): 2298-2306.doi: 10.3864/j.issn.0578-1752.2013.11.014

• HORTICULTURE • Previous Articles     Next Articles

Influence of Exogenous ALA and Spd on Photosynthesis and Antioxidant System of Low Temperature and Poor Light Affected Pepper Seedlings

 ZHANG  Fan, YU  Ji-Hua, JIE  Jian-Ming, FENG  Zhi, ZHANG  Guo-Bin, LI  Wen-Lin   

  1. College of Agronomy，Gansu Agricultural University，Lanzhou 730070
  • Received:2012-07-02 Online:2013-06-01 Published:2013-04-18

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Abstract: 【Objective】 The objective of this experiment is to investigate the effect of 5-aminolevulinic acid (ALA) and spermidine (Spd) on the hot pepper (Capsicum annuum L.) and its mechanism with seedling leaves at low temperature and poor light. 【Method】 The effect of Spd and ALA on continuing cold resistance of hot pepper was investigated by using hot pepper seedlings as the test materials under low temperature (15℃/5℃) (day/night) and poor light (20-30 μmol•m-2•s-1) adverse circumstances simulated by artificial climate chamber with foliar spray by ALA (0.5 mg•L-1) and Spd (0.5 mmol•L-1). 【Result】 The results showed that the treatment with Spd and ALA alleviated the decline of the maximal photochemical efficiency (Fv/Fm) of photosystem II(PSII), the actual photochemical efficiency of photosystem II (ΦPSII), photochemical quenching coefficient (qP) and the efficiency of light energy capture by open PSII reaction centers (Fv′/Fm′) under low temperature and dim light adverse circumstances, also significantly increased the net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr) and the activity of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), and alleviated continual enhancement of MDA content and membrane permeability. The content of soluble protein and proline was positively correlated with tolerance. 【Conclusion】 The increasing cold resistance of hot pepper with ALA and Spd treatment under critical low temperature and dim light adverse circumstances is related to activated defensive system and protected cell membrane. Additionally, the increasing activity of SOD, POD and CAT also could promote metabolism of active oxygen to protect photosynthesis of cell.

Key words: pepper , ALA , Spd , photosynthetic characteristics , antioxidant systems , low temperature and poor light

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