Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (5): 927-933.doi: 10.3864/j.issn.0578-1752.2013.05.007

• PLANT PROTECTION • Previous Articles     Next Articles

Determination and Characterization of Extracellular Melanin from Setosphaeria turcica and Influencing Factors of Its Production

 WU  Nan, LI  Qing-Wei, CAO  Zhi-Yan, ZHANG  Jiao, HAO  Zhi-Min, DONG  Jin-Gao   

  1. 1.College of Life Science, Agricultural University of Hebei/Mycotoxin and Molecular Plant Pathology Laboratory,Baoding 071000, Hebei
    2.Institute of Botany, Chinese Academy of Sciences, Beijing 100093
  • Received:2012-11-02 Online:2013-03-01 Published:2012-12-14

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Abstract: 【Objective】The objective of this study is to determine the type of extracellular melanin from Setosphaeria turcica and explore the influencing factors of its production to lay a foundation for further clarification of the relationship between melanin and pathogenicity. 【Method】 Acid precipitation method was used to extract the extracellular melanin of 01-23 and ΔSt3hnr-3 strain. The type of melanin was preliminarily identified with infrared spectroscopy. Media containing tyrosine, tricyclazole, Cu2+ and different pH values were used to determine the influences of these factors on the production of extracellular melanin.【Result】The infrared spectroscopy of extracellular melanin in S. turcica was different from the DHN melanin, while the physical and chemical properties of the samples from both strains were similar. It could dissolve in hot alkaline solution and produce precipitation when it was reacted with FeCl3. Under the shaking culture, the contents of extracellular melanin in the culture filtrates respectively containing tyrosine and tricyclazole were about 2 and 1.5 times more than that of the mock, while it could also be slightly enhanced by Cu2+ with the concentration lower than 0.5 μmol•L-1 or at pH6.0. 【Conclusion】The extracellular melanin from S. turcica was proved to be similar with DOPA melanin. Tyrosine and tricyclazole obviously promoted the production of extracellular melanin.

Key words: Setosphaeria turcica , DOPA melanin , infrared spectroscopy

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