Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (5): 934-942.doi: 10.3864/j.issn.0578-1752.2013.05.008

• PLANT PROTECTION • Previous Articles     Next Articles

Morphological and Molecular Identification of Cereal Cyst Nematodes on Wheat from Jiangsu Province of China

 WANG  Xuan, LE  Xiu-Hu, SONG  Zhi-Qiang, XIANG  Gui-Lin, LIN  Yu, LI  Hong-Mei   

  1. College of Plant Protection, Nanjing Agricultural University/Key Laboratory of Monitoring and Management of Disease and Insects, Ministry of Education, Nanjing 210095
  • Received:2012-08-29 Online:2013-03-01 Published:2012-12-06

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Abstract: 【Objective】The objective of this study is to understand the species of cereal cyst nematodes (CCN) from Jiangsu Province and their genetic relationships, and to provide valuable information for screening resistant wheat cultivars, plant breeding and integrated control of the disease. 【Method】 The morphology and morphometrics for cyst, vulval cone and second stage juvenile were characterized for 13 typical Jiangsu populations. The ITS regions of ribosomal DNA were amplified and the PCR products were sequenced. A phylogenetic tree was constructed based on the ITS sequences.【Result】The results demonstrated that 13 Jiangsu populations were similar with those described populations of Heterodera avenae by comparing the morphological characters and morphometric data. The phylogenetic tree revealed that the Jiangsu populations were grouped with domestic and abroad populations of H. avenae into a big branch, which could clearly separate Chinese populations from foreign populations.【Conclusion】All the studied CCN populations from Jiangsu Province were identified as H. avenae. The variations in morphological characters and ITS sequences among different Jiangsu populations were very small. The status of H. filipjevi in Jiangsu Province is unknown.

Key words: cereal cyst nematode , species identification , Heterodera avenae , rDNA-ITS , phylogeny

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