高渗胁迫对玉米大斑病菌生长发育的影响及菌丝细胞中渗透调节物质的分析

doi:10.3864/j.issn.0578-1752.2017.10.017

Abstract

Abstract: 【Objective】The objective of this study is to understand the effect of hyperosmotic stress on the growth and development, melanin content in mycelium cells, determine the probable osmolytes in the mycelium cells and to clarify the variation rule of these substances under different hyperosmotic stress conditions in Setosphaeria turcica. 【Method】The effects of hyperosmotic stress on colony growth rate, mycelium morphological characteristics and melanin content in S. turcica mycelium cell were analyzed under different hyperosmotic stress media, in which 0.4, 0.8, and 1.2 mol·L^-1 NaCl were added in PDA medium. High performance liquid chromatography (HPLC) technology was employed to detect the content of polyhydroxy-alcohol including glycerol, erythrol, glucose, mannitol, trehalose and analyzed the change profiles of these substances as time increased. 【Result】Compared to the strain cultured on PDA medium, the strain cultured under hyperosmotic stress treatments appeared with decreased in colony growth rate, shortened septum and swollen cells. Melanin content in mycelium cells cultured under hyperosmotic stress treatments in all time points showed a significant difference compared to the control group, in which melanin content in the samples treated before 12 h were lower than that in control, but there were no obvious differences between the samples treated. Moreover, there were different effects under different times (24, 48 h) and different concentration treatments. Compared to the control group, melanin content in the samples treated with 1.2 mol·L^-1 NaCl was significantly increased, melanin content treated with 0.8 mol·L^-1 NaCl was significantly decreased, melanin content in samples treated with 0.4 mol·L^-1 NaCl was significantly decreased in 24 h, but the content in the samples after 48 h treatment was almost the same with the control. Mannitol contents in mycelium cell under different hyperosmotic stress treatments were increased with the increase in time, especially the mannitol content significantly increased after 36 h treatment. There was a similar change tendency in glycerol and mannitol contents in mycelium cells under different treatments. Glycerol content increased most obviously compared with the control group and the content was significantly increased after 24 h treatment. Trehalose content in mycelium cells showed an increasing tendency as time increased, and the tendency increased with the increase of stress intensity, however, trehalose content after 36 h treatment was significantly lower than that in control group, while no significant difference was found in the changes of erythrol and glucose contents. No mannitol, trehalose and glycerol were detected in culture filtrate, while glucose content had no significant change no matter the fact that the mycelium was cultured in PDA medium or under hyperosmotic stress treatments.【Conclusion】 The colony growth rate was inhibited, mycelium cell was swollen, and septum was shortened under hyperosmotic stress. There was a significant inhibition on mannitol content before 12 h treatment. Mannitol and glycerol were main osmolytes in mycelium cells. Trehalose was also involved in hyperosmotic stress reaction in S. turcica.

Key words: Setosphaeria turcica;hyperosmotic stress, growth and development, osmolyte

GONG XiaoDong, LIU XingChen, ZHAO LiQing, ZHENG YaNan, FAN YongShan, HAN JianMin, GU ShouQin, DONG JinGao. Effect of Hyperosmotic Stress on Growth and Development of Setosphaeria turcica and Determination of Osmolytes in the Mycelium Cells of the Pathogen[J].Scientia Agricultura Sinica, 2017, 50(10): 1922-1929.

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References

[1] KUSAI N A, AZMI M M, ZAINUDIN N A, YUSOF M T, RAZAK A A. Morphological and molecular characterization, sexual reproduction, and pathogenicity of Setosphaeria rostrata isolates from rice leaf spot. Mycologia, 2016, 108(5): 905-914.

[2] 于舒怡, 傅俊范, 周如军, 康晓军, 刘博. 不同栽培模式对玉米大斑病发生和流行的影响. 玉米科学, 2011, 19(1): 132-135.

Yu S Y, Fu J F, Zhou R J, Kang X J, Liu B. Effects of different cultivation patterns on the occurrence and epidemic of northern leaf blight on maize. Maize Science, 2011, 19(1): 132-135. (in Chinese)

[3] Dong J G, Fan Y S, Gui X M, An X L, Ma J F, Dong Z P. Geographic distribution and genetic analysis of physiological races of Setosphaeria turcica in Northern China. American Journal of Agricultural and Biological Science, 2008, 3(1): 389-398.

[4] Shankhdhar D, Shankhdhar S C, Pant R C. Osmoprotectants: an overview. Physiology and Molecular Biology of Plants, 2004, 10(2): 167-180.

[5] Ruijter G J, Bax M, Patel H, Flitter S J, van de Vondervoort P J, de Vries R P, vanKuyk P A, Visser J. Mannitol is required for stress tolerance in Aspergillus niger conidiospores. Eukaryot Cell, 2003, 2(4): 690-698.

[6] Wyatt T T, van Leeuwen M R, Wösten H A, Dijksterhuis J. Mannitol is essential for the development of stress-resistant ascospores in Neosartorya fischeri (Aspergillus fischeri). Fungal Genetics and Biology, 2014, 64(2): 11-24.

[7] Dixon K P, Xu J R, Smirnoff N, Talbot N J. Independent signaling pathways regulate cellular turgor during hyperosmotic stress and appressorium-mediated plant infection by the rice blast fungus Magnaporthe grisea. The Plant Cell, 1999, 11(10): 2045-2058.

[8] Duran R, Cary J W, Calvo A M. Role of the osmotic stress regulatory pathway in morphogenesis and secondary metabolism in filamentous fungi. Toxins (Basel), 2010, 2(4): 367-381.

[9] DUNLAP C A, JACKSON M A, SAHA B C. Compatible solutes of sclerotia of Mycoleptodiscus terrestris under different culture and drying conditions. Biocontrol Science and Technology, 2011, 21(1): 113-123.

[10] KAYINGO G, WONG B. The MAP kinase Hog1p differentially regulates stress-induced production and accumulation of glycerol and D-arabitol in Candida albicans. Microbiology, 2005, 151(9): 2987-2999.

[11] Yan H, Jia L H, Lin Y P, Jiang N. Glycerol accumulation in the dimorphic yeast Saccharomycopsis ?buligera: cloning of two glycerol 3-phosphate dehydrogenase genes, one of which is markedly induced by osmotic stress. Yeast, 2008, 25(9): 609-621.

[12] Gori K, Mortensen H D, Arneborg N, Jespersen L. Expression of the GPD1 and GPP2 orthologues and glycerol retention during growth of Debaryomyces hansenii at high NaCl concentrations. Yeast, 2005, 22(15): 1213-1222.

[13] Thorsen M, Di Y, Tängemo C, Morillas M, Ahmadpour D, VanDer Does C, Wagner A, Johansson E, Boman J, Posas F, Wysocki R, Tamas M J. The MAPK Hog1p modulates Fps1p-dependent arsenite uptake and tolerance in yeast. Molecular Biology of the Cell, 2006, 17(10): 4400-4410.

[14] Bashyal B M, Ramesh C, Chanda K, Devyani S, Prasad L C, Joshi A K. Association of melanin content with conidiogenesis in Bipolaris Sorokiniana of barley (Hordeum vulgare L.) . World Journal of Microbiology and Biotechnology, 2010, 26(2): 309-316.

[15] WOOD J M. Bacterial osmoregulation: a paradigm for the study of cellular homeostasis. Annual Review of Microbiology, 2011, 65: 215-238.

[16] SAITO H, POSAS F. Response to hyperosmotic stress. Genetics, 2012, 192(2): 289-318.

[17] BURG M B, FERRARIS J D. Intracellular organic osmolytes: function and regulation. Journal of Biological Chemistry, 2008, 283(12): 7309-7313.

[18] HOHMANN S, KRANTZ M, NORDLANDER B. Yeast osmoregulation. Methods in Enzymology, 2007, 428: 29-45.

[19] WESTFALL P J, PATTERSON J C, CHEN R E, Thorner J. Stress resistance and signal fidelity independent of nuclear MAPK function. Proceedings of the National Academy of Sciences of the United States of America, 2008, 105(34): 12212-12217.

[20] DE Nadal E, AMMERER G, POSAS F. Controlling gene expression in response to stress. Nature Reviews Genetics, 2011, 12(12): 833-845.

[21] DAVIS D J, BURLAK C, MONEY N P. Osmotic pressure of fungal compatible osmolytes. Mycological Research, 2000, 104(7): 800-804.

[22] HOHMANN S. Osmotic stress signaling and osmoadaptation in yeasts. Microbiology and Molecular Biology Reviews, 2002, 66(2): 300-372.

[23] KIEWIETDEJONGE A, PITTS M, CABUHAT L, SHERMAN C, KLADWANG W, MIRAMONTES G, FLORESVILLAR J, CHAN J, RAMIREZ R M. Hypersaline stress induces the turnover of phosphatidylcholine and results in the synthesis of the renal osmoprotectant glycerophosphocholine in Saccharomyces cerevisiae. FEMS Yeast Research, 2006, 6(2): 205-217.

[24] KELLY D J A，BUDD K. Polyol metabolism and osmotic adjustment in the mycelial ascomycete Neocosmospora vasinfecta (E. F. Smith). Experimental Mycology, 1991, 15(1): 55-64.

[25] ABADIAS M, TEIXIDO N, USALL J, VINAS I, MAGAN N. Solute stresses affect growth patterns, endogenous water potentials and accumulation of sugars and sugar alcohols in cells of the biocontrol yeast Candida sake. Journal of Applied Microbiology, 2000, 89(6): 1009-1017.

[26] NESCI A, ETCHEVERRY M, MAGAN N. Osmotic and matric potential effects on growth, sugar alcohol and sugar accumulation by Aspergillus section Flavi strains from Argentina. Journal of Applied Microbiology, 2004, 96(5): 965-972.

[27] KOGEI T, STEIN M, VOLKMANN M, GORBUSHINA A A, GALINSKI E A, GUNDE-CIMERMAN N. Osmotic adaptation of the halophilic fungus Hortaea werneckii: role of osmolytes and melanization. Microbiology, 2007, 153(12): 4261-4273.

[28] Diano A, Bekker-Jensen S, Dynesen J, Nielsen J. Polyol synthesis in Aspergillus niger: influence of oxygen availability, carbon and nitrogen sources on the metabolism. Biotechnology & Bioengineering, 2006, 94(5): 899-908.

[29] Voegele R T, Hahn M, Lohaus G, Link T, Heiser I, Mendgen K. Possible roles for mannitol and mannitol dehydrogenase in the biotrophic plant pathogen Uromyces fabae. Plant Physiology, 2005, 137(1): 190-198.

[30] Vélëz H, Glassbrook N J, Daub M E. Mannitol biosynthesis is required for plant pathogenicity by Alternaria alternata. FEMS Microbiology Letters, 2008, 285(1): 122-129.

[31] Calmes B, Guillemette T, Teyssier L, Siegler B, Pigné S, Landreau A, Iacomi B, Lemoine R, Richomme P, Simoneau P. Role of mannitol metabolism in the pathogenicity of the necrotrophic fungus Alternaria brassicicola. Frontiers in Plant Science, 2013, 4(2): 131.

[32] MEENA M, PRASAD V, Zehra A, Gupta V K, Upadhyay R S. Mannitol metabolism during pathogenic fungal-host interactions under stressed conditions. Frontiers in Microbiology, 2015, 6: 1019.

[33] Clark A J, Blissett K J, Oliver R P. Investigating the role of polyols in Cladosporium fulvum during growth under hyper-osmotic stress and in planta. Planta, 2002, 216(4): 614-619.

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Effect of Hyperosmotic Stress on Growth and Development of Setosphaeria turcica and Determination of Osmolytes in the Mycelium Cells of the Pathogen

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