Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (19): 3996-4003.doi: 10.3864/j.issn.0578-1752.2012.19.011

• PLANT PROTECTION • Previous Articles     Next Articles

Cloning and Bioinformatics Analysis of Voltage-Gated Sodium Channel Gene cDNA in Bactrocera dorsalis (Hendel)

 JIANG  Xuan-Zhao, WEI  Dan-Dan, SHEN  Guang-Mao, DOU  Wei, WANG  Jin-Jun   

  1. 1.西南大学植物保护学院昆虫学及害虫控制工程重庆市市级重点实验室，重庆 400715
  • Received:2012-02-07 Online:2012-10-01 Published:2012-03-23

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Abstract: 【Objective】 The objective of this study is to clone voltage-gated sodium channel gene (VGSC) from Bactrocera dorsalis (Hendel), and to identify its typical hallmarks. It will provide basic molecular information for clarifying insecticide resistance mechanism of B. dorsalis.【Method】 The cDNA sequence was isolated using RT-PCR and PCR methods. Based on the sequencing results, the bioinformatics analysis of nucleic acid and putative amino acid was conducted.【Result】An almost full-length cDNA sequence (6 446 bp) of VGSC was obtained, including a complete open reading frame (ORF) of 6 405 bp, which encoded 2 134 amino acids and included all the typical hallmarks of VGSC. The amino acid shared 91.7%, 86.9% and 42.3% identity with sodium channel genes of Drosophila melanogaster (NP_001188635), Musca domestica (AAB47604), and Homo sapiens Nav1.2 (NP_066287), respectively. 【Conclusion】A complete ORF of VGSC was sequenced with clone strategy from oriental fruit fly for the first time. Abundant alternative splicing phenomena existed in the VGSC of B. dorsalis. Three potential mutation points, which may confer resistance to pyrethroids and DDT, were observed. It is suggested that VGSC would be used as a molecular marker in phylogenetic study of insect.

Key words: Bactrocera dorsalis, sodium channel, gene cloning, bioinformatics analysis, phylogenetic analysis

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