Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (17): 3624-3631.doi: 10.3864/j.issn.0578-1752.2012.17.020

• RESEARCH NOTES • Previous Articles     Next Articles

Functional Analysis of Cyclophilin (GhCYP1) in Gossypium hirsutum

 WU  Li, LI  Yan-Jun, ZHANG  Xin-Yu, WANG  Ya-Qin, SUN  Jie   

  1. 1.石河子大学生命科学学院，新疆石河子 832003
    2.石河子大学农学院/新疆兵团绿洲生态农业重点实验室，新疆石河子 832003
  • Received:2012-03-26 Online:2012-09-01 Published:2012-05-28

PDF

724

Cited

1

Abstract: 【Objective】The objective of this study is to investigate the effects of cotton GhCYP1 gene on drought tolerance of tobacco.【Method】The plant expression vector of GhCYP1 gene was constructed by using gateway cloning technology and transformed into tobacco based on Agrobacterium tumerficiens-mediated transformation approach. Transgenic tobacco plants fused the target genes were generated. Wild type (WT), transgenic lines L1 and L2 of tobacco were used to measure the relative content of chlorophyll in leaf discs, relative water content, malonyldialdehyde content and relative electrical conductivity in plants.【Result】Compared with WT, transgenic tobacco plants form a stronger root system. Under the conditions of water stress, the leaf chlorophyll was less affected in transgenic plants which had obviously higher leaf chlorophyll content in leaf discs. The 3rd day after water stress，relative water content, the malonyldialdehyde content and relative electrical conductivity in L1 and L2 transgenic lines showed no difference with that in WT. After 13 d under water stress conditions, relative water content in transgenic lines was significantly higher than in WT, but the malonyldialdehyde content and relative electrical conductivity were significantly lower than in WT. 【Conclusion】These results suggested that WT tobacco plants were greatly affected under water stress conditions, and the transgenic plants were less affected. Overexpression of GhCYP1 improved the drought tolerance of transgenic tobacco plants.  

Key words: cotton, tobacco, GhCYP1 gene, genetic transformation, drought tolerance

[1]刘金定, 叶武威, 樊宝相. 我国棉花抗逆研究及其利用. 中国棉花, 1998, 25(3): 5-6.

Liu J D, Ye W W, Fan B X. Cotton resilience and utilization in China. Chinese Cotton, 1998, 25(3): 5-6. (in Chinese)

[2]Chaves M M, Maroco J P, Pereira J S. Understanding plant responses to drought from genes to the whole plant. Functional Plant Biology, 2003, 30: 239-264.

[3]Galat A. Peptidylproline cis-trans-isomerases: Immunophilins. European Journal of Biochemistry, 1993, 216(3): 689-707.

[4]Chen A P, Wang G L, Qu Z L, Lu C X, Liu N, Wang F, Xia G X. Ectopic expression of ThCYP1, a stress-responsive cyclophilin gene from Thellungiella halophila, confers salt tolerance in fission yeast and tobacco cells. Plant Cell Reports, 2007, 26(2): 237-245.

[5]Kong H Y, Lee S C, Hwang B K. Expression of pepper cyclophilin gene is diferentially regulated during the pathogen infection and abiotic stress conditions. Physiological and Molecular Plant Pathology, 2001, 59(4): 189-199.

[6]Cho E K, Kim M. A red algal cyclophilin has an effect on development and growth in Nicotiana tabacum. Plant Physiology and Biochemistry, 2008, 46(10): 868-874.

[7]Ruan S L, Ma H S, Wang S H, Fu Y P, Xin Ya, Liu W Z, Wang F, Tong J X, Wang S Z, Chen H Z. Proteomic identification of OsCYP2, a rice cyclophilin that confers salt tolerance in rice (Oryza sativa L.) seedlings when overexpressed. BMC Plant Biology, 2011, 11(34): 2-15.

[8]Kim S K, You YN, Park J C, Joung Y, Kim B G, Ahn J C, Cho H S. The rice thylakoid luminal cyclophilin OsCYP20-2 confers enhanced environmental stress tolerance in tobacco and Arabidopsis. Plant Cell Reports, 2012, 31(2): 417-426.

[9]郭  芳, 李艳军, 张新宇, 吴  莉, 孙  杰. 棉花亲环素基因(GhCYP1)克隆及在干旱胁迫下的表达分析. 分子植物育种, 2010, 8(2): 265-270.

Guo F, Li Y J, Zhang X Y, Wu L, Sun J. Cotton cyclophilin gene GhCYP1 cloning and expression analysis under drought stress. Molecular Plant Breeding, 2010, 8(2): 265-270. (in Chinese)

[10]Buchholz W G, Harrishaller L, DeRose R T. Cyclophillins are encoded by a small gene family in rice. Plant Molecular Biology, 1994, 25: 837-843.

[11]Oh K, Ivanchenko M G, White T J, Lomax T L. The diageotropica gene of tomato encodes a cyclophilin: A novel player in auxin signaling. Planta, 2006, 224(1): 133-144.

[12]Marivet J, Frendo P, Burkard G. DNA sequence analysis of a cyclophiln gene from maize: Developmental expression and regulation by salicylic acid. Molecular Genetics and Genomics ,1995, 247(2): 222-228.

[13]Gan P H, Shan W, Blackman L M, Hardham A R. Characterization of cyclophilin-encoding genes in phytophthora. Molecular Genetics and Genomics, 2009, 281(2): 565-578.

[14]Godoy A V, Lazzaro A S, Casalongue C A, San S B. Expression of a Solanum tuberosum cyclophilin gene is regulated by fungal infection and abiotic stress conditions. Plant Science, 2000, 152: 123-134.

[15]Kan Y C, Liu S W, Guo Z J. Characterization of a cyclophilin cDNA from soybean cell. Acta Botanica Sinice, 2002, 44(2): 173-176.

[16]蒋建雄, 张天真. 利用CTAB/酸酚法提取棉花组织总RNA. 棉花学报, 2003, 15(3): 166-167.

Jiang J X, Zhang T Z. Use of CTAB/acid phenol extraction of total RNA of cotton. Cotton Science, 2003, 15(3): 166-167. (in Chinese)

[17]Horsch R B, Fry J, Hoffman N L, Wallroth M, Eichholtx D, Rogers S G, Fraley R T. A simple and general method for transferring genes into plants. Science, 1985, 227: 1229-1231.

[18]奥斯伯,布伦特. 精编分子生物学实验指南. 北京: 北京科学出版社, 1999.

Aosbo F M, Bulut R. Molecular Biology Laboratory Manual for Fine. Beijing: Beijing Science Press, 1999. (in Chinese)

[19]刘  宁. 盐芥(Thellungiella halophila)耐盐基因的规模化功能性筛选及小立宛藓(Physcomitrella patens)耐逆基因PpDBF1的分离和功能鉴定[D]. 北京: 中国科学院, 2007.

Liu N. Functional screening of salt-stress related genes from Thellungiella halophila and characterization of stress-inducible gene PpDBF1 in Physcomitrella patens[D]. Beijing:  Chinese Academy of Sciences, 2007.

[20]李合生. 植物生理生化实验原理和技术. 北京: 北京高等教育出版社, 2003: 164-263.

Li H S. Plant Physiological and Biochemical Principles and Experimental Techniques. Beijing: Beijing Higher Education Press, 2003: 164-263. (in Chinese)

[21]Dominguez-Solis J R, He Z, Lima A, Ting J, Buchanan B B, Luan S. A cyclophilin links redox and light signals to cysteine biosynthesis and stress responses in chloroplasts. Proceedings of the National Academy of Sciences of the USA, 2008, 105(42): 16386-16391.

[22]Cho E K, Kim M. A red algal cyclophilin has an effect on development and growth in Nicotiana tabacum. Plant Physiology and Biochemistry, 2008(10): 868-874.

[23]Li H, He Z, Lu G, Lee S C, Alonso J, Ecker J R, Luan S. A WD40 domain cyclophilin interacts with histone H3 and functions in gene repression and organogenesis in Arabidopsos. The Plant Cell, 2007, 19(8): 2403-2416.

[24]朱维琴, 吴良欢, 陶勤南. 作物根系对干旱胁迫逆境的适应性研究进展. 土壤与环境, 2002, 11(4): 430-433.

Zhu W Q, Wu L H, Tao Q N. Advances in the studies on crop root against drought stress. Soil and Environmental Sciences, 2002, 11(4): 430-433. (in Chinese)

[25]Zhu C F, Wang Y W, Li Y B, Bhatti K H, Tian Y C, Wu J H. Overexpression of a cotton cyclophilin gene(GhCyp1) in transgenic tobacco plants confers dual tolerance to salt stress and Pseudomonas syringae pr.. Tabaci Infection, 2011, 49: 1264-1271.
[1] LI YuanJing, YUAN RuiXiang, LI YongTai, SUN TianGe, LIU Feng, LI YanJun, ZHANG XinYu. Identification and Functional Characterization of β-Glucosidase Genes in Verticillium dahliae for Pathogenicity on Cotton [J]. Scientia Agricultura Sinica, 2026, 59(7): 1380-1399.
[2] YAN TingLin, DU YaDan, HU XiaoTao, WANG He, LI XiaoYan, WANG YuMing, NIU WenQuan, GU XiaoBo. The Impacts of Nitrogen Fertilizer Organic Alternatives Under Aerated Drip Irrigation on Cotton Yield and Water Use Efficiency Under Deficit Irrigation Conditions [J]. Scientia Agricultura Sinica, 2026, 59(3): 602-618.
[3] YANG KeXin, ZHANG Yong, LI YanXiu, XIE SiYao, XUE Bo, YANG ShaoJie, SONG DeWei, MA Qiang, ZOU Ping, LI Yang, MA SiQi, JING ChangLiang. Effects of Alginate Oligosaccharides on Alleviating Atrazine Phytotoxicity in Tobacco [J]. Scientia Agricultura Sinica, 2026, 59(1): 101-113.
[4] GUO ChenLi, LIU Yang, CHEN Yan, HU Wei, WANG YouHua, ZHOU ZhiGuo, ZHAO WenQing. Effects of Phosphorus Fertilizer Postpone Under Nitrogen Reduction Condition on Yield, Phosphorus Fertilizer Utilization Efficiency of Drip-Irrigated Cotton [J]. Scientia Agricultura Sinica, 2025, 58(9): 1749-1766.
[5] WANG WeiMeng, WEI YunXiao, TANG YunNi, LIU MiaoMiao, CHEN QuanJia, DENG XiaoJuan, ZHANG Rui. Establishment and Rooting Optimization of Agrobacterium rhizogenes Transformation System in Cotton [J]. Scientia Agricultura Sinica, 2025, 58(8): 1479-1493.
[6] ZHAO YuXuan, MIAO JiYuan, HU Wei, ZHOU ZhiGuo. Effects of Low Temperature at Seedling Stage on Cotton Floral Bud Differentiation and Cotton Plant Yield [J]. Scientia Agricultura Sinica, 2025, 58(7): 1311-1320.
[7] TIAN LiWen, LOU ShanWei, ZHANG PengZhong, DU MingWei, LUO HongHai, LI Jie, PAHATI MaiMaiTi, MA TengFei, ZHANG LiZhen. Analysis of Problems and Pathways for Increasing Cotton Yield per Unit Area in Xinjiang Under Green and Efficient Production Mode [J]. Scientia Agricultura Sinica, 2025, 58(6): 1102-1115.
[8] WANG WenJuan, SHI ShangLi, KANG WenJuan, DU YuanYuan, YIN Chen. The Physiological Response of Longzhong Alfalfa to Exogenous Spermine Under Drought Stress [J]. Scientia Agricultura Sinica, 2025, 58(4): 676-691.
[9] ZHENG Yu, CHEN Yi, TI JinSong, SHI LongFei, XU XiaoBo, LI YuLin, GUO Rui. Evaluation of Carbon Footprint and Economic Benefit of Different Tobacco Rotation Patterns [J]. Scientia Agricultura Sinica, 2025, 58(4): 733-747.
[10] WANG LiYuan, WANG Hui, WANG MuMu, WANG DongJian, LI RuYu, ZHENG YongSheng, ZHANG Han. Construction and Application of DNA Fingerprint Database for Known Varieties in Upland Cotton DUS Testing [J]. Scientia Agricultura Sinica, 2025, 58(22): 4570-4588.
[11] CHEN YongXian, CHEN RuiJiang, DU YiZhi, ZHU JunJie, CHEN WanXia, ZHAO ZiHan, WANG JiChun, DU Kang, ZHANG Kai. Screening and Identification of Drought-Tolerant Sweet Potato Germplasm Resources [J]. Scientia Agricultura Sinica, 2025, 58(2): 214-237.
[12] CHEN JuanNi, CHEN PinLu, LI Yu, XIE MengXiao, LI XinBei, DING Wei. Mechanism of Tobacco Resistance to Bacterial Wilt Induced by Magnesium Oxide Nanoparticles [J]. Scientia Agricultura Sinica, 2025, 58(16): 3327-3344.
[13] TANG ChaoYuan, LIU TaoFen, WU YanQin, ZHANG QiPeng, LI ZiLiang, CHEN YunRui, LEI ZhangYing, ZHANG YaLi, ZHANG WangFeng, DU MingWei, YANG MingFeng, TIAN JingShan. Relationship Between Boll Morphological Characteristics and Fiber and Kernel Quality of Gossypium hirsutum L. and Gossypium barbadense L. [J]. Scientia Agricultura Sinica, 2025, 58(15): 2980-2992.
[14] WEN Jin, NING YanFang, QIN Xin, LIU Yuan, ZHANG XiaoLing, ZHU YongHong, TIAN ShiMin, MA YanBin. Resistance Evaluation and Genetic Stability Analysis of Insect- Resistant and Glyphosate-Tolerant Transgenic Cotton Lines [J]. Scientia Agricultura Sinica, 2025, 58(12): 2291-2302.
[15] DONG Ming, QI Hong, ZHANG Qian, WANG Yan, WANG ShuLin, FENG GuoYi, LIANG QingLong, GUO BaoSheng. The Impact of Sowing Methods on the Seed Germination Environment and Cotton Emergence and Growth [J]. Scientia Agricultura Sinica, 2025, 58(12): 2346-2357.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd