Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (16): 3391-3398.doi: 10.3864/j.issn.0578-1752.2012.16.019

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Influence of High Temperature on Oxidative Damage and Follicle Development of Laying Hens

 YANG  Xiao-Lan, ZHANG  Min-Hong, FENG  Jing-Hai, DI  Li-Li, JIANG  Li-Wen   

  1. 中国农业科学院北京畜牧兽医研究所/动物营养学国家重点实验室，北京100193
  • Received:2012-02-24 Online:2012-08-15 Published:2012-05-08

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Abstract: 【Objective】 Two experiments were conducted to assess the relationship between follicle development of heat stressed laying hens and oxidative stress induced by high temperature, and then to reveal the mechanism of lower egg production of heat stressed laying hens.【Method】In experiment 1, 180 Hy-line Brown hens, 27 wk of age, were randomly divided into three groups and housed into three controlled environment chambers. Three groups were respectively normal thermal group (NT group: 28℃, basal diet), daily cyclic high temperature group (HT group: 28-35-28℃, basal diet) and daily cyclic high temperature and supplemented with Vitamin E (VE) group (HT+VE group: 28-35-28℃, basal diet supplemented with 250 IU•kg-1 VE).  In experiment two, granulosa cells dissociated from F1 and F2 follicles were incubated under either 39℃ or 44℃. 【Result】The results of experiment 1 showed that daily cyclic high temperature significantly inhibited follicle development of laying hens, which mainly manifested as decreasing in egg production (P＜0.05), ovary weight (P＜0.05) and large follicle number (P＜0.05) as well as inhibition in P450 cholesterol side-chain cleavage enzyme (P450scc ) mRNA expression (P＜0.05) and progesterone (P4) production (P＜0.05) in granulosa cell. The results also showed that daily cyclic high temperature induced hens oxidative stress, which manifested as higher malondialdehyde (MDA) concentration (P＜0.05) in plasma, liver tissue and egg yolk. When exposed to cyclic high temperature, 250 IU•kg-1 VE supplementation decreased MDA concentration significantly in plasma (P＜0.05), liver tissue (P＜0.05), egg yolk (P＜0.05), and alleviated (P＜0.05) inhibition in follicle development of laying hens. The results of experiment 2 showed that high temperature increased reactive oxygen species (ROS) level (P＜0.05) in granulosa cells and decreased proliferation of granulosa cells (P＜0.05). VE supplemented into medium alleviated the negative effects of chronic heat stress, manifested as decreasing in ROS level (P＜0.05) and increasing in proliferation of granulosa cells (P＜0.05). 【Conclusion】 The results demonstrated that heat stress increased ROS level in granulosa cell and induced hens (liver, plasma and egg yolk) oxidative stress, in addition, heat stress inhibited P4 production, proliferation of granulosa cells and follicle development of laying hen, while VE depressed oxidative damage induced by heat stress, promoted follicle development of heat stressed laying hens. All the results suggest that the mechanism of poor follicle development of heat stressed hens involves in oxidative stress induced by high temperature.

Key words: high temperature, follicle development, granulosa cells, oxidative damage, laying hen

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