Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (16): 3399-3405.doi: 10.3864/j.issn.0578-1752.2012.16.020

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Effect of Plant Essential Oil on the Distribution of Urea-Nitrogen in Rumen Contents

 ZHAO  Sheng-Guo, WANG  Jia-Qi, BU  Deng-Pan, ZHOU  Ling-Yun, SUN  Peng   

  1. 中国农业科学院北京畜牧兽医研究所/动物营养学国家重点实验室，北京 100193
  • Received:2011-12-05 Online:2012-08-15 Published:2012-03-05

PDF

722

Cited

1

Abstract:  【Objective】 The objective of the experiment is to reveal the distribution of urea-nitrogen in rumen contents and the effect of essential oil on the distribution. 【Method】 Garlic oil, tea tree oil and eucalyptus oils were added into serum bottles containing rumen fluid and 15N-urea. The abundance of 15N and rumen fermentation were detected after incubation. The bacterial population was studied by DGGE.【Result】 Urea nitrogen in rumen fluid decreased and that in the microbes associated with rumen solid and liquid phases increased following the incubation time, and then had the similar level at 24 h. Compared to the control, urea nitrogen abundance in the microbes associated with rumen solid and liquid phases treated with garlic oil increased by 22.60% and 18.75%, pH and ammonia nitrogen decreased by 3.02% and 17.80%, respectively. The proportion of isobutyric acid and acetic acid: propionic acid increased at 24 h. The pH in rumen fluid treated with tea tree and eucalyptus oils decreased (P＜0.05), and the proportion of isobutyric acid and acetic acid:propionic acid increased. Three different essential oils affected bacterial population according to the results by DGGE.【Conclusion】 Urea-nitrogen was transferred from rumen fluid to microbes, and garlic oil inhibited the transfer, changed microbial population and decreased ammonia production.

Key words: rumen contents, urea, essential oil, microbial population

[1]Bloomfield R A, Garner G B, Muhrer M E. Kinetics of urea metabolism in sheep. Journal of Animal Science, 1960, 19: 1248.

[2]Moharrery A, Das T K. Correlation between microbial enzyme activities in the rumen fluid of sheep under different treatments. Reproduction Nutrition Development, 2001, 41(6): 513-529.

[3]赵圣国, 王加启, 刘开朗, 李  旦, 于  萍, 卜登攀. 荷斯坦奶牛瘤胃微生物脲酶的分离与鉴定. 畜牧兽医学报, 2010, 41(6): 692-696.

Zhao S G, Wang J Q, Liu K L, Li D, Yu P, Bu D P. Isolation and identification of urease from the rumen content of Holstein cows by a culture-independent strategy. Acta Veterinaria et Zootechnica Sinica, 2010, 41(6): 692-696. (in Chinese)

[4]Benchaar C, Calsamiglia S, Chaves A V, Fraser G R, Colombatto D, McAllisterc T A, Beauchemin K A. A review of plant-derived essential oils in ruminant nutrition and production. Animal Feed Science and Technology, 2008, 145(1/4): 209-228.

[5]Macheboeuf D, Morgavi D P, Papon Y, Mousset J L, Arturo-Schaan M. Dose–response effects of essential oils on in vitro fermentation activity of the rumen microbial population. Animal Feed Science and Technology, 2008, 145(1/4): 335-350.

[6]Spanghero M, Robinson P H, Zanfi C, Fabbro E. Effect of increasing doses of a microencapsulated blend of essential oils on performance of lactating primiparous dairy cows. Animal Feed Science and Technology, 2009, 153(1/2): 153-157.

[7]王洪荣, 郝志敏, 李世霞, 秦  韬, 王梦芝. 植物次生代谢产物对瘤胃发酵调控的研究进展. 动物营养学报, 2010, 22 (6): 1471-1476.

Wang H R, He Z M, Li S X, Qin T, Wang M Z. Recent advances in plants secondary metabolites and their application as rumen fermentation manipulating agents in ruminants. Acta Zoonutrimenta Sinica, 2010, 22(6): 1471-1476. (in Chinese)

[8]Santos M B, Robinson P H, Williams P, Losa R. Effects of addition of an essential oil complex to the diet of lactating dairy cows on whole tract digestion of nutrients and productive performance. Animal Feed Science and Technology, 2010, 157(1/2): 64-71.

[9]Juszkiewicz A, Zaborska W, Sepio? J, Góra M, Zaborska A. Inactivation of jack bean urease by allicin. Journal of Enzyme Inhibition and Medicinal Chemistry, 2003, 18(5): 419-424.

[10]Hassani A R, Ordouzadeh N, Ghaemi A, Amirmozafari N, Hamdi K, Nazari R. In vitro inhibition of Helicobacter pylori urease with non and semi fermented Camellia sinensis. Indian Journal of Medical Microbiology, 2009, 27(1): 30-34.

[11]陆  燕, 林  波, 王  恬, 石放雄, 刘建新. 大蒜油对体外瘤胃发酵、甲烷生成和微生物区系的影响. 动物营养学报, 2010, 22(2): 386-392.

Lu Y, Lin B, Wang T, Shi F X, Liu J X. Effects of garlic oils addition on rumen fermentation, methanogenesis and microbiota in vitro. Acta Zoonutrimenta Sinica, 2010, 22(2): 386-392. (in Chinese)

[12]Larue R, Yu Z, Parisi V A, Egan A R, Morrison M. Novel microbial diversity adherent to plant biomass in the herbivore gastrointestinal tract, as revealed by ribosomal intergenic spacer analysis and rrs gene sequencing. Environmental Microbiology, 2005, 7(4): 530-543.

[13]Weatherburn M W. Phenol-hypochlorite reaction for determination of ammonia. Analytical Chemistry, 1967, 39(8): 971-974.

[14]Liu L, Wang J Q, Bu D P, Liu S J, Liang S, Wei H Y, Zhou L Y, Liu K L. Short communication: malic acid does not promote vaccenic acid accumulation in mixed ruminal fluid with fractionated fish oil by a rumen-simulation technique. Journal of Dairy Science, 2008, 91(10): 3993-3997.

[15]Yu Z, Morrison M. Improved extraction of PCR-quality community DNA from digesta and fecal samples. Biotechniques, 2004, 36(5): 808-812.

[16]冯  薇, 王加启, 刘开朗, 卜登攀, 李  旦, 赵圣国, 杨开伦. 运用PCR-DGGE分析比较瘤胃中不同饲料固相粘附微生物区系. 畜牧兽医学报, 2010, 41(12): 1556-1562.

Feng W, Wang J Q, Liu K L, Bu D P, Li D, Zhao S G, Yang K L. Comparison of microbial communities adherent to three kinds of feeds in dairy cows by PCR-DGGE. Acta Veterinaria et Zootechnica Sinica, 2010, 41(12): 1556-1562. (in Chinese)

[17]Taylor-Edwards C C, Elam N A, Kitts S E, McLeod K R, Axe D E, Vanzant E S, Kristensen N B, Harmon D L. Influence of slow-release urea on nitrogen balance and portal-drained visceral nutrient flux in beef steers. Journal of Animal Science, 2009, 87(1): 209-221.

[18]Pope P B, Smith W, Denman S E, Tringe S G, Barry K, Hugenholtz P, McSweeney C S, McHardy A C, Morrison M. Isolation of succinivibrionaceae implicated in low methane emissions from tammar wallabies. Science, 2011, 333(6042): 646-648.

[19]Newbold C J, McIntosh F M, Williams P, Losa R Wallace R J. Effects of a specific blend of essential oil compounds on rumen fermentation. Animal Feed Science and Technology, 2004, 114(1/4): 105-112.

[20]Lodhi M A, Hussain J, Abbasi M A, Jassbi A R, Choudhary M I, Ahmad V U. A new Bacillus pasteurii urease inhibitor from Euphorbia decipiens. Journal of Enzyme Inhibition and Medicinal Chemistry, 2006, 21(5): 531-535.

[21]Patra A K. Effects of essential oils on rumen fermentation, Microbial Ecology and ruminant production. Asian Journal of Animal and Veterinary Advances, 2011, 6(5): 416-428.

[22]Castilljos L, Calsamiglia S, Ferret A, Losa R. Effects of dose and adaptation time of a specific blend of essential oil compounds on rumen fermentation. Animal Feed Science and Technology, 2007, 132(3/4): 186-201.

[23]Cardozo P W, Calsamiglia S, Ferret A, Kamel C. Screening for the effects of natural plant extracts at different pH on in vitro rumen microbial fermentation of a high-concentrate diet for beef cattle. Journal of Animal Science, 2005, 83(11): 2572-2579.

[24]Ross Z M, O'Gara E A, Hill D J, Sleightholme H V, Maslin D J. Antimicrobial properties of garlic oil against human enteric bacteria: Evaluation of methodologies and comparisons with garlic oil sul?des and garlic powder. Applied and Environmental Microbiology, 2001, 67(1): 475-480.

[25]Cox S D, Mann C M, Markham J L, Bell H C, Gustafson J E, Warmington J R, Wyllie S G. The mode of antimicrobial action of the essential oil of Melaleuca alternifolia (tea tree oil). Journal of Applied Microbiology, 2000, 88(1): 170-175.

[26]Calsamiglia S, Busquet M, Cardozo P W, Castillejos L, Ferret A. Invited review: essential oils as modifiers of rumen microbial fermentation. Journal of Dairy Science, 2007, 90(6): 2580-2595.
[1] YAN YanGe, ZHANG ShuiQin, LI YanTing, ZHAO BingQiang, YUAN Liang. Effects of Dextran Modified Urea on Winter Wheat Yield and Fate of Nitrogen Fertilizer [J]. Scientia Agricultura Sinica, 2023, 56(2): 287-299.
[2] ZHANG YingQiang,ZHANG ShuiQin,LI YanTing,ZHAO BingQiang,YUAN Liang. Conversion Characteristics of Different Carboxyl-Containing Organic Acids Modified Urea in Calcareous Fluvo-Aquic Soil [J]. Scientia Agricultura Sinica, 2022, 55(17): 3355-3364.
[3] JING JianYuan,YUAN Liang,ZHANG ShuiQin,LI YanTing,ZHAO BingQiang. The Characteristics of Ammonia Volatilization of Humic Acid-Urea Complex Combined with Urea from Different Fertilized Soils [J]. Scientia Agricultura Sinica, 2022, 55(14): 2786-2796.
[4] XU FangLei,ZHANG Jie,LI Yang,ZHANG WeiWei,BO QiFei,LI ShiQing,YUE ShanChao. Effects of Fertilization Methods on Ammonia Volatilization of Spring Maize in Dry Farming on the Loess Plateau [J]. Scientia Agricultura Sinica, 2022, 55(12): 2360-2371.
[5] ZHAO LiFang, YUAN Liang, ZHANG ShuiQin, ZHAO BingQiang, LIN ZhiAn, LI YanTing. Effects of Zinc Combined with Urea on Zinc Availability and Urea Conversion [J]. Scientia Agricultura Sinica, 2021, 54(16): 3461-3472.
[6] DING XiangPeng,LI GuangHao,ZHANG JiWang,LIU Peng,REN BaiZhao,ZHAO Bin. Effects of Base Application Depths of Controlled Release Urea on Yield and Nitrogen Utilization of Summer Maize [J]. Scientia Agricultura Sinica, 2020, 53(21): 4342-4354.
[7] ZHU CongHua, ZHANG YuPing, XIANG Jing, ZHANG YiKai, WU Hui, WANG YaLiang, ZHU DeFeng, CHEN HuiZhe. Effects of Side Deep Fertilization on Yield Formation and Nitrogen Utilization of Mechanized Transplanting Rice [J]. Scientia Agricultura Sinica, 2019, 52(23): 4228-4239.
[8] FENG XiaoJie,ZHAN XiuMei,WANG XueXin,CHEN Kun,PENG Jing,HAN XiaoRi. Effects of Soil Inorganic Nitrogen and Nitrogen Absorbing by Maize Under the Reduced Application of Coated Urea at Different Proportions [J]. Scientia Agricultura Sinica, 2019, 52(10): 1733-1745.
[9] WANG Yin, XU Zhuo, LI BoNing, GAO Qiang, FENG GuoZhong, LI CuiLan,YAN Li, WANG ShaoJie. Effects of Urea Ammonium Nitrate Solution on Grain Yield and Nitrogen Uptake of Spring Maize in Black Soil Region [J]. Scientia Agricultura Sinica, 2018, 51(4): 718-727.
[10] HaiTao LIN, Yan LI, ZhaoHui LIU, YuWen SHEN, LiHua JIANG, DeShui TAN, XiaoZong SONG, Ping LIU. The Match of the Nitrogen Uptake in Winter Wheat and Nitrogen Release of Water Borne Resin Coated Urea [J]. Scientia Agricultura Sinica, 2018, 51(20): 3852-3862.
[11] PengFei LI, XiaoKun LI, WenFeng HOU, Tao REN, RiHuan CONG, ChangWen DU, LieHuo XING, ShaoHua WANG, JianWei LU. Studying the Fate and Recovery Efficiency of Controlled Release Urea in Paddy Soil Using 15N Tracer Technique [J]. Scientia Agricultura Sinica, 2018, 51(20): 3961-3971.
[12] Mu ZHANG, ShuanHu TANG, QiaoYi HUANG, YuWan PANG, Qiong YI, Xu HUANG, Ping LI, HongTing FU. The Nutrient Supply Characteristics of Co-Application of Slow-Release Urea and Common Urea in Double-Cropping Rice [J]. Scientia Agricultura Sinica, 2018, 51(20): 3985-3995.
[13] QiaoYi HUANG, Mu ZHANG, Xu HUANG, ShuanHu TANG, FaBao ZHANG, YuWan PANG, Qiong YI, Ping LI, HongTing FU. Effect of One-off Application of Poly Urea-Formaldehyde Fertilizer Under Reduced N Rate on Double Cropping Rice [J]. Scientia Agricultura Sinica, 2018, 51(20): 3996-4006.
[14] ZHANG YaWei, LIU QiuXia, ZHU DanDan, FAN XiaoLin, REN Tao, ZHANG LiMei, LI XiaoKun, CONG RiHuan, LU JianWei. Effects of Different Special Controlled Release Urea Dosages on Yield and Nitrogen Uptake of Oilseed Rape [J]. Scientia Agricultura Sinica, 2018, 51(1): 139-148.
[15] GUO JinJin, ZHANG FuCang, WANG HaiDong, YAN ShiCheng, ZHENG Jing, CHEN DongFeng, LI ZhiJun. Effects of Slow-Release Nitrogen Fertilizer and Urea Blending on Maize Growth and Nitrogen Uptake Under Different Nitrogen Application Rates [J]. Scientia Agricultura Sinica, 2017, 50(20): 3930-3943.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd