纳米铜对大鼠肝脏毒性相关蛋白过氧化氢酶的分离鉴定及生物信息学分析

doi:10.3864/j.issn.0578-1752.2012.14.022

Abstract

Abstract: 【Objective】In order to investigate the hepatotoxic mechanisms of nanoparticles copper, catalase (CAT) was isolated and identified from liver, and analyzed by bioinformatics, which is related with hepatotoxity induced by copper nanoparticles in rats.【Method】 The differential expression proteins related with hepatotoxity of copper nanoparticles were screened by 2-DE and PDQuest 8.0 software and then analyzed by bioinformatics after identified by MALDI-TOF-TOF MS through comparative proteomics strategy.【Result】The 6602 and 7702 spots of differentially expressed proteins were found to associate with hepatotoxity. They were identified as CAT protein which was located in the cytoplasm. This hydrophilic protein had no signal peptide, and was non-secreted protein. It also contained catalase active sites 64FDRERIPERVVHAKGAG80 and catalase heme ligand sites 354RLFAYPDTH362. The random coils, α-helices and extended chains were its main secondary structural elements, and the three-dimensional structure was predicted. Homology analysis showed that CAT had a high homology between rat and the other eight species, and the phylogenetic tree of CAT was constructed. 【Conclusion】Copper nanoparticles could regulate down the CAT protein expression so as to induce oxidative stress injury in liver cells, which may be a pathway of copper nanoparticles to exert the hepatotoxic effects in rats.

Key words: copper nanoparticles, hepatotoxity, proteomics, catalase, bioinformatics, rat

DONG Shu-Wei, GAO Zhao-Hui, SHEN Xiao-Yun, XUE Hui-Wen, LI Xia. Isolation, Identification and Bioinformatics Analysis of CAT Protein Related with Hepatotoxity by Copper Nanoparticles in Rats[J].Scientia Agricultura Sinica, 2012, 45(14): 2981-2990.

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Isolation, Identification and Bioinformatics Analysis of CAT Protein Related with Hepatotoxity by Copper Nanoparticles in Rats

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