Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (13): 2658-2667.doi: 10.3864/j.issn.0578-1752.2012.13.010

• SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT • Previous Articles     Next Articles

Effects of Earthworms Collected from South China on Soil Enzyme Activities and Microbial Characteristics

 ZHANG  Chi, CHEN  Xu-Fei, ZHOU  Bo, LI  Jian-Long, YANG  Cheng-Fang, DAI  Jun   

  1. 1.华南农业大学资源环境学院，广州 510642
    2.广东省农业科学院茶叶研究所，广东英德 513042
  • Received:2011-11-25 Online:2012-07-01 Published:2012-03-24

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Abstract: 【Objective】Effects of two earthworm species on soil microbial characteristics and enzyme activities were studied in paddy and vegetable soils in South China. 【Method】Two earthworm species (Amynthas robustus and Amynthas corticis) were cultured in paddy and vegetable soils in the lab. Parameters such as soil catalase, urease, invertase, acid and alkaline phosphatase, soil basal respiration, microbe biomass carbon, microbial metabolic quotient were analyzed to evaluate earthworm’s effects on soil biological quality in different soil types. 【Result】Results showed that A. corticis could significantly improve soil basal respiration, enhance catalase and ureased activities, and decrease acid phosphatase activities (P＜0.05) in paddy soil. In vegetable soil, both of earthworm species increase urease activities. A. robustus and A. corticis increased invertase and catalase activities (P＜0.05), respectively. Multivariate analysis showed that A. corticis and A. robustus improved soil biological properties in both of two kinds of soil, and especially more positive effects on soil biological quality were shown in A. corticis than A. robustus in vegetable soil. 【Conclusion】Earthworms improved soil biological quality in the study, so it could be considered as a biological resource for the application in sustainable soil management. Effects of earthworms on soil biological quality depend on their ecological categories and soil properties. It is recommended that more studies on the screening of earthworm species and organic matter additions should be performed in future according to the local conditions.

Key words: A. corticis, A. robustus, paddy soil, vegetable garden soil, soil microbial characteristics, soil enzyme activities

[1]Gil-Stores F, Trasar-Cepeda C, Leirós M C, Seoane S. Different approaches to evaluating soil quality using biochemical properties. Soil Biology and Biochemistry, 2005, 37: 877-887.

[2]Bossio D A, Fleck J A, Scow K M, Fujii R. Alteration of soil microbial communities and water quality in restored wetlands. Soil Biology and Biochemistry, 2006, 38: 1223-1233.

[3]Lagomarsino A, Moscatelli M C, Tizio A D, Mancinelli R, Grego S, Marinari S. Soil biochemical indicators as a tool to assess the short- term impact of agricultural management on changes in organic C in a Mediterranean environment. Ecological Indicators, 2009, 9: 518-527.

[4]Brown G G, Doube B M. The functional interactions between earthworms, microorganisms, organic matter, and plant//Ewards C A. Earthworm Ecology, 2nd Edition. Boca Raton: CRC Press, 2004: 213-240.

[5]Varma A, Oelmuller R. Advanced Techniques in Soil Microbiology. Berlin/Heidelberg/New York: Springer-Verlag, 2007: 201-208.

[6]张宝贵, 李贵桐, 申天寿. 威廉环毛蚯蚓对土壤微生物量及活性的影响. 生态学报, 2000, 20(1): 168-172.

Zhang B Q, Li G T, Shen T S. Influence of the earthworm Pheretima guillelmi on soil microbial biomass and activity. Acta Ecologica Sinica, 2000, 20(1):168-172. (in Chinese)

[7]Aira M, Monroy F, Domínguez J. Changes in microbial biomass and microbial activity of pig slurry after the transit through the gut of the earthworm Eudrilus eugeniae (Kinberg, 1867). Biology and Fertility of Soils, 2006, 42: 371-372.

[8]Gómez-Brandón M, Lazcano C, Lores M, Domínguez J. Detritivorous earthworms modify microbial community structure and accelerate plant residue decomposition. Applied Soil Ecology, 2010, 44: 237-244.

[9]Mora P, Miambi E, Jiménez J J, Decaëns T, Rouland C. Functional complement of biogenic structures produced by earthworms, termites and ants in the neotropical savannas. Soil Biology and Biochemistry, 2005, 37: 1043-1048.

[10]胡  锋, 王  霞, 李辉信, 于建光, 王丹丹. 蚯蚓活动对稻麦轮作系统中土壤微生物量碳的影响. 土壤学报, 2005, 42(6): 965-969.

Hu F, Wang X, Li H X, Yu J G, Wang D D. Effects of earthworms on soil microbial biomass carbon in rice-wheat rotation agro-ecosystem. Acta Pedologica Sinica, 2005, 42(6): 965-969. (in Chinese)

[11]McLean M A, Migge-Kleian S, Parkinson D. Earthworm invasions of ecosystems devoid of earthworms: effects on soil microbes. Biological Invasions, 2006, 8: 1257-1273.

[12]Tao J, Griffiths B, Zhang S J, Chen X Y, Liu M Q, Hu F, Li H X. Effects of earthworms on soil enzyme activity in an organic residue amended rice-wheat rotation agro-ecosystem. Applied Soil Ecology, 2009, 42: 221-226.

[13]Jouquet P, Bernard-Reversat F, Bottinelli N, Orange D, Rouland-Lefevre C, Duc T T, Podwojewski P. Influence of changes in land use and earthworm activities on carbon and nitrogen dynamics in a steepland ecosystem in Northern Vietnam. Biology and Fertility of Soils, 2007, 44: 69-77.

[14]Vance E D, Brookes P C, Jenkinson D S. An extraction method for measuring soil microbial biomass C. Soil Biology and Biochemistry, 1987, 19(6): 703-707.

[15]Sparling G P. Ratio of microbial biomass carbon to soil organic carbon as a sensitive indicator of changes in soil organic matter. Australia Journal of Soil Research, 1992, 30: 195-207.

[16]Wang W J, Dalal R C, Moody P W, Smith C J. Relationships of soil respiration to microbial biomass, substrate availability and clay content. Soil Biology and Biochemistry, 2003, 35: 273-284.

[17]Zibilsk L M. Carbon mineralization//Weaver R W, Angle S, Bottomley P, Bezdicek D, Smith S. Methods of Soil Analysis Part2-Microbiological and Biochemical Properties. Soil Science Society of America, 1994: 835-859.

[18]Anderson T H. Microbial eco-physiological indicators to assess soil quality. Agriculture, Ecosystems and Environment, 2003, 98: 285-293.

[19]关松荫. 土壤酶及其研究法. 北京: 农业出版社, 1986: 234-279.

Guan S Y. Soil Enzyme and Its Methods. Beijing: Agricultural Press, 1986: 234-279. (in Chinese)

[20]The R Development Core Team. R: A Language and Environment for Statistical Computing. Vienna, Austria : R Foundation for Statistical Computing, 2007.

[21]Thioulouse J, Chessel D, Doledec S, Olivier J M. ADE-4: a multivariate analysis and graphical display software. Statistics and Computing, 1997, 7: 75-83.

[22]Fierer N, Schimel J P, Holden P A. Variations in microbial community composition through two soil depth profiles. Soil Biology and Biochemistry, 2003, 35(1): 167-176.

[23]刘  婷, 任宗玲, 张  池, 陈旭飞, 周  波, 戴  军. 蚯蚓堆置处理对农业有机废弃物的化学及生物学影响的主成分分析. 应用生态学报, 2012, 23(3): 779-784.

Liu T, Ren Z L, Zhang C, Chen X F, Zhou B, Dai J. Effects of composting with earthworm on the chemical and biological properties of agricultural organic wastes: A principal component analysis. Chinese Journal of Applied Ecology, 2012, 23(3): 779-784. (in Chinese)

[24]刘德辉, 胡  锋, 胡 佩. 蚯蚓活动对红壤磷素有效性的影响及其活化机理研究. 生态学报, 2003, 23(11): 2299-2306.

Liu D H, Hu F, Hu P. Influence of earthworm activities on phosphorus availability of red soil and activated mechanism induced by earthworm. Acta Ecologica Sinica, 2003, 23(11): 2299-2306. (in Chinese)

[25]Lee K E. Earthworms: Their Ecology and Relationships with Soils and Land Use. Sydney: Academic Press, 1985: 224-226.

[26]Salmon S. Earthworm excreta (mucus and urine) affect the distribution of springtails in forest soils. Biology and Fertility of Soils, 2001, 34: 304-310.

[27]Lavelle P, Spain A V. Soil Ecology. Dordrecht/Boston/London: Kluwer Academic Publishers, 2001.

[28]Wolters V. Invertebrate control of soil organic matter stability. Biology and Fertility of Soils, 2000, 31: 1-19.

[29]Zhang B G, Li G T, Shen T S, Wang J K, Sun Z. Changes in microbial biomass C, N, and P and enzyme activities in soil incubated with earthworms Metaphire guillelmi or Eisenia fetida. Soil Biology and Biochemistry, 2000, 32: 2055-2062.

[30]Insam H, Mitchell C C, Dormaar J F. Relationship of soil microbial biomass and activity with fertilization practice and crop yield of three ultisols. Soil Biology and Biochemistry, 1991, 23: 459-464.

[31]Dilly O, Munch J C. Microbial biomass content, basal respiration and enzyme activities during the course of decomposition of leaf litter in a black alder (Alnus glutinosa (L.) Gaertn. ) forest. Soil Biology and Biochemistry, 1996, 28(8): 1073-1081.

[32]He Z L, Yang X E, Baligar V C, Calvert D V. Microbiological and biochemical indexing systems for assessing quality of acid soils. Advances in Agronomy, 2003, 78: 89-138.

[33]Sen B, Chandra T S. Do earthworms affect dynamics of functional response and genetic structure of microbial community in a lab-scale composing system? Bioresource Technology, 2009, 100: 804-811.

[34]Aira M, Domínguez J. Microbial and nutrient stabilization of two animal manures after the transit through the gut of the earthworm Eisenia fetida (Savigny, 1826). Journal of Hazardous Materials, 2009, 161: 1234-1238.

[35]Kizilkaya R, Hepsen S. Microbiological properties in earthworm cast and surrounding soil amended with various organic wastes. Communications in Soil Science and Plant Analysis, 2007, 38: 2861-2876.

[36]Olander L P, Vitousek P M. Regulation of soil phosphatase and chitinase activity by N and P availability. Biogeochemistry, 2000, 49: 175-190.

[37]Le Bayon R C, Binet F. Earthworms change the distribution and availability of phosphorous in organic substrates. Soil Biology and Biochemistry, 2006, 38: 235-246.

[38]Six J, Elliott E T, Paustian K. Soil macroaggregate turnover and microaggregate formation: a mechanism for C sequestration under no-tillage agriculture. Soil Biology and Biochemistry, 2000, 32: 2099- 2103.

[39]Ernst G, Henseler I, Felten D, Emmerling C. Decomposition and mineralization of energy crop residues governed by earthworms. Soil Biology and Biochemistry, 2009, 41: 1548-1554.
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